Food and Fermentation Industries >

2021 , Vol. 47 >Issue 20: 30 - 36

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026788

Effect of exogenous amino acids on the rejuvenation of degraded strains of Volvariella volvacea

  • KONG Zixuan ,
  • WANG Qiaoli ,
  • CHENG Zhihong ,
  • YUN Jianmin ,
  • ZHANG Wenwei ,
  • ZHAO Fengyun
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  • (College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China)

Received date: 2021-01-17

  Revised date: 2021-03-19

  Online published: 2021-11-18

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Abstract

Amino acid is the basic unit of protein, which is closely related to life activities. In this study, 20 kinds of amino acids were added to the culture medium to rejuvenate the degenerative strains of Volvariella volvacea. Serine, Planine, Valine, Leucine and Proline had good rejuvenation effect, and further study was carried out. The results showed that after adding 5 kinds of amino acids, the growth rate and biomass of V. volvacea strain D1-D3 were increased, the contents of polysaccharides, proteins, flavonoids and polyphenols were increased, the accumulation of reactive oxygen species was effectively inhibited and the activity of antioxidant enzymes was improved; the rejuvenation effect was Ser > Ala > Val > Leu > Pro. Compared with the control group, the mycelial growth rate and biomass of D3 were increased by 7% and 10%, respectively, and the contents of polysaccharides, proteins, flavonoids and polyphenols increased 17%, 40%, 3%, 14%, respectively. The contents of ·O-2, H2O2 decreased by 22% and 37%, while the activities of peroxidase, superoxide dismutase, catalase and glutathione peroxidase increased by 46%, 60%, 167% and 92%, respectively. The results showed that the mycelial characteristics of degenerative strains of V. volvacea could be effectively rejuvenated by adding Ser into the medium. This study provides theoretical basis and technical support for the rejuvenation of V. volvacea and other edible fungi.

Key words： Volvariella volvacea; strain degradation; amino acid; rejuvenation; Serine

Cite this article

KONG Zixuan , WANG Qiaoli , CHENG Zhihong , YUN Jianmin , ZHANG Wenwei , ZHAO Fengyun . Effect of exogenous amino acids on the rejuvenation of degraded strains of Volvariella volvacea[J]. Food and Fermentation Industries, 2021 , 47(20) : 30 -36 . DOI: 10.13995/j.cnki.11-1802/ts.026788

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