Food and Fermentation Industries >

2021 , Vol. 47 >Issue 20: 253 - 259

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026668

Matrix effect in analysis of 24 veterinary drug residues in animal-derived foods by QuEChERS-UPLC-MS/MS

  • LI Hongli ,
  • WU Xiaoqin ,
  • ZHAO Bo ,
  • HOU Meiling ,
  • WU Yanlei ,
  • HUANG Xianliang
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  • (Chongqing Institute for Food and Drug Control, Chongqing 401120,China)

Received date: 2021-01-20

  Revised date: 2021-03-11

  Online published: 2021-11-18

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Abstract

The accuracy and sensitivity of the result can be influenced by matrix effects. By evaluating ion suppression rate, the matrix effects of 24 veterinary drugs in carp, chicken, duck gizzard, pork liver, beef and pork were systematically evaluated in this study. The compensation effect on the matrix effect by using isotope internal standard and the matrix-matched standard curve were also been evaluated. The results showed that sulfamethoxine, sulfadoxine, ciprofloxacin, enrofloxacin, norfloxacin, erythromycin and amantadine were appropriate for the isotope internal standard calibration method. When adopting this method, the matrix effects of these 7 veterinary drugs decreased by 0.15%-66.94% (n=3) in 6 kinds of the matrix. The other 17 kinds of veterinary drugs were appropriate for adapting the matrix-matched standard curve method to calibrate matrix effect since the recoveries of the 17 kinds of veterinary drugs increased by 11.71%-59.47% (n=3) after being calibrated by blank pork matrix-matched standard curve. The adverse interference of quantification results caused by the matrix effect was successfully compensated by both methods. This study provides a reference for the rapid and accurate detection of veterinary drug residues in animal-derived food.

Key words： ultra performance liquid chromatography-mass spectrometry; multiple veterinary drug residue; matrix effect; ion suppression; animal-derived foods

Cite this article

LI Hongli , WU Xiaoqin , ZHAO Bo , HOU Meiling , WU Yanlei , HUANG Xianliang . Matrix effect in analysis of 24 veterinary drug residues in animal-derived foods by QuEChERS-UPLC-MS/MS[J]. Food and Fermentation Industries, 2021 , 47(20) : 253 -259 . DOI: 10.13995/j.cnki.11-1802/ts.026668

References

[1] SCHNEIDER M J, LEHOTAY S J, LIGHTFIELD A R.A multiclass multiresidue LC-MS/MS method for analysis of veterinary drugs in bovine kidney[J].Progress in Energy & Combustion Science,2012:515-519.
[2] FRENICH A G, AGUILERA-LUIZ M D,VIDAL J L M, et al.Comparison of several extraction techniques for multiclass analysis of veterinary drugs in eggs using ultra-high pressure liquid chromatography-tandem mass spectrometry[J].Analytica Chimica Acta,2010, 661(2):150-160.
[3] 郭海霞,肖桂英,张禧庆,等.QuEChERS-超高效液相色谱-串联质谱法同时检测猪肉中121种兽药[J].色谱, 2015, 33(12):1 242-1 250.
GUO H X, XIAO G Y, ZHANG X Q, et al.Simultaneous determination of 121 veterinary drugs in pork by QuEChERS and ultra performance liquid chromatography-tandem mass spectrometry[J].Chinese Journal of Chromatography, 2015, 33(12):1 242-1 250.
[4] KINSELLA B, WHELAN M, CANTWELL H, et al.A dual validation approach to detect anthelmintic residues in bovine liver over an extended concentration range[J].Talanta, 2011,83(1):14-24.
[5] 冯晓杰, 杜丽英, 冯章英, 等.LC-MS/MS法测定人血药浓度的基质效应研究进展[J].中国新药杂志,2015, 24(13):1 488-1 492.
FENG X J, DU L Y, FENG Z Y, et al.Research progress of matrix effect in determining blood concentration by LC-MS/MS[J]. Chinese Journal of New Drugs, 2015, 24(13):1 488-1 492.
[6] 苏萌, 艾连峰.液相色谱-串联质谱基质效应及其消除方法[J].食品安全质量检测学报,2014, 5(2):511-515.
SU M, AI L F.Matrix effects and elimination methods of liquid chromatography-tandem mass spectrometry[J].Journal of Food Safety and Quality,2014, 5(2):511-515.
[7] JANUSCH F, KALTHOFF L, HAMSCHER G, et al.Evaluation and subsequent minimization of matrix effects caused by phospholipids in LC-MS analysis of biological samples[J].Bioanalysis, 2013, 5(17):2 101-2 114.
[8] LIU D H, WU Y L, WANG B, et al.Analysis of five types of veterinary drug residues in meat products by dispersive solid phase extraction-UPLC-MS/MS[J].Modern Food Science and Technology, 2016, 32(10):290-296.
[9] SUI T, FU J, LI X Y, et al.Simultaneous determination of veterinary drug residues in aquatic products by UPLC-MS/MS[J].Food Science, 2011.DOI:10.3724/SP.J.1011.2011.00211.
[10] HOFF R B, RÜBENSAM G, JANK L, et al.Analytical quality assurance in veterinary drug residue analysis methods:Matrix effects determination and monitoring for sulfonamides analysis[J].Talanta, 2015, 132:443-450.
[11] ZHAO L M, LUCAS D, LONG D, et al.Multi-class multi-residue analysis of veterinary drugs in meat using enhanced matrix removal lipid cleanup and liquid chromatography-tandem mass spectrometry[J].Journal of Chromatography A, 2018,1 549:14-24.
[12] ZHANG N, FAN S, XUE Y, et al.Rapid detection of 8 veterinary drug residues in chicken by QuEChERS-ultra performance liquid chromatography tandem mass spectrometry[J].Journal of Hygiene Research, 2017, 46(1):89-93.
[13] MALLET C R, LU Z, MAZZEO J R.A study of ion suppression effects in electrospray ionization from mobile phase additives and solid-phase extracts[J].Rapid Communications in Mass Spectrometry, 2010, 18(1):49-58.
[14] ANTIGNAC J P, WASCH K D, MONTEAU F, et al.The ion suppression phenomenon in liquid chromatography-mass spectrometry and its consequences in the field of residue analysis[J].Analytica Chimica Acta, 2005, 529(1-2):129-136.
[15] ISMAIEL O A, HALQUIST M S, ELMAMLY M Y, et al.Monitoring phospholipids for assessment of ion enhancement and ion suppression in ESI and APCI LC/MS/MS for chlorpheniramine in human plasma and the importance of multiple source matrix effect evaluations[J].Journal of Chromatography B Analytical Technologies in the Biomedical & Life Sciences,2008, 875(2):333-343.
[16] 王大鹏, 张娴, 颜昌宙.高效液相色谱串联质谱法测定污水污泥中4种磺胺类药物及其乙酰化代谢物[J].环境化学, 2018, 37(10):2 143-2 151.
WANG D P, ZHANG X, YAN C Z.Determination of four sulfonamides and their corresponding acetyl metabolites in wastewater and sludge by high performance liquid chromatography tandem mass spectrometry[J].Nvironmental Chemistry, 2018, 37(10):2 143-2 151.
[17] 唐吉旺, 袁列江, 肖泳, 等.固相萃取-高效液相色谱-质谱联用法同时测定食品中9种人工合成甜味剂[J].色谱, 2019, 37(6):619-625.
TANG J W, YUAN L J, XIAO Y, et al.Simultaneous determination of nine artificial sweeteners in foodby solid phase extraction coupled with high performanceliquid chromatography tandem mass spectrometry[J].Chinese Journal of Chromatography, 2019, 37(6):619-625.
[18] 邱增枝, 郑增忍, 赵思俊, 等.UPLC-MS/MS法测定猪尿中β2-受体激动剂的基质效应研究[J].动物医学进展, 2013, 34(9):66-70.
QIU Z Z, ZHENG Z R, ZHAO S J,et al.Matrix effects in the residue detection of β2-agonists in pig urine by UPLC-MS/MS[J].Progress in Veterinary Medicine, 2013, 34(9):66-70.
[19] 周悦榕, 李丹妮, 吴剑平, 等.超高效液相色谱-电喷雾电离-串联质谱法测定猪粪便中6种抗生素残留的基质效应研究[J].分析测试学报, 2017, 36(8):1 010-1 017.
ZHOU Y R, LI D N, WU J P.Study on matrix effects in analysis of six antibiotics in swine manure by ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry[J].Journal of Instrumental Analysis, 2017, 36(8):1 010-1 017.
[20] XU R N, FAN L, RIESER M J, et al.Recent advances in high-throughput quantitative bioanalysis by LC-MS/MS[J].Journal of Pharmaceutical & Biomedical Analysis, 2007, 44(2):342-355.
[21] HERNÁNDEZ F, SANCHO J V, POZO O J.Critical review of the application of liquid chromatography/mass spectrometry to the determination of pesticide residues in biological samples[J].Analytical and Bioanalytical Chemistry, 2005, 382(4):934-946.
[22] 王立琦, 贺利民, 曾振灵, 等.液相色谱-串联质谱检测兽药残留中的基质效应研究进展[J].质谱学报, 2011, 32(6):321-332.
WANG L Q, HE L M, ZENG Z L, et al.Progress in matrix effect of veterinary drug residues analysis by high-performance liquid chromatography tandem mass spectrometry[J]. Journal of Chinese Mass Spectrometry Society, 2011, 32(6):321-332.
[23] TAYLOR P J.Matrix effects:The achilles heel of quantitative high-performance liquid chromatography-electrospray-tandem mass spectrometry[J].Clinical Biochemistry, 2005, 38(4):328-334.
[24] SEAN O, ILETT K F.Evaluation of a deuterium-labeled internal standard for the measurement of sirolimus by high-throughput HPLC electrospray ionization tandem mass spectrometry[J].Clinical Chemistry, 2008, 54(8):1 386-1 389.
[25] 张凯, 秦宇, 卞华, 等.QuEChERS-超高效液相色谱-串联质谱法检测羊肉中8种抗真菌药[J].色谱, 2018, 36(10):999-1 004.
ZHANG K, QIN Y, BIAN H, et al.Determination of eight antifungal drugs in mutton by QuEChERS coupled with ultra-performance liquid chromatography-tandem mass spectrometry[J] Chinese Journal of Chromatography, 2018, 36(10):999-1 004.
[26] 李丽春, 刘书贵, 尹怡, 等.QuECHRS结合UPLC-MS/MS法测定水产品中酰胺醇类抗生素残留及基质效应[J].中国渔业质量与标准,2018,8(6):32-39.
LI L C, LIU S G, YIN Y, et al. The amphenicols residues analysis in aquatic products and their matrix effects by QuECHERS method and UPLC-MS/MS[J].Chinese Fishery Quality and Standards, 2018, 8(6):32-39.
[27] 李红丽. QuEChERS-UPLC-MS/MS同时测定动物性食品中24种残留兽药方法及基质效应的研究[D].重庆:西南大学, 2020.
LI H L.Study on simultaneous determination of 24 veterinary drug residues contained in animal derived foods and the matrix effect with QuEChERS-UPLC-MS/MS[D].Chongqing:Southwest University, 2020.
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