Bacterial diversity and odor characteristics of four typical cheeses in Xinjiang

  • WANG Jin ,
  • ZHANG Jingjing ,
  • LI Xiaochen ,
  • NING Xibin ,
  • LUO Lei ,
  • JIANG Min ,
  • LI Xiaohui
Expand
  • 1(College of Food Sciences, Shanghai Ocean University, Shanghai 201306, China)
    2(Shanghai Aquatic Product and Processing and Storage Engineering Technology Research Center, Shanghai 201306, China)
    3(Laboratory for Risk Assessment of Quality and Safety of Storage and Preservation of Aquatic Products, Ministry of Agriculture,Shanghai 201306, China)

Received date: 2021-02-20

  Revised date: 2021-04-01

  Online published: 2021-11-18

Abstract

In this study, the bacterial diversity and volatile components in four Xinjiang cheeses were investigated using Illumina MiSeq sequencing technology and solid-phase microextraction (SPME) with gas chromatography-mass spectrometry (GC-MS), respectively. The results showed that Lactobacillus, Streptococcus and Lactococcus were the dominant genera in cow milk cheese and sheep milk cheese, while Acetobacter and Lactobacillus were dominant in acid milk cheese. A total of 68 volatile compounds were identified, including esters, acids, alcohols, aldehydes ketones etc. The main aroma compounds of cow milk cheese were esters and ketones, the major flavor profile were wine aroma, fruity, and grassy odors. The main aroma compounds of sheep milk cheese were esters and aromatic heterocycles, the major flavor profile were wine aroma, fruity, sweet and grassy odors. The main aroma compounds of acid milk cheese were esters, and the major flavor profile were wine aroma, fruity, grassy and creamy odors. The Pearson correlation analysis of bacterial communities and volatile flavor compounds revealed that Lactobacillus, Streptococcus and Acetobacter might play important roles on flavor production in Xinjiang cheeses.

Cite this article

WANG Jin , ZHANG Jingjing , LI Xiaochen , NING Xibin , LUO Lei , JIANG Min , LI Xiaohui . Bacterial diversity and odor characteristics of four typical cheeses in Xinjiang[J]. Food and Fermentation Industries, 2021 , 47(20) : 285 -290 . DOI: 10.13995/j.cnki.11-1802/ts.027060

References

[1] 努尔古丽·达嘎尓别克. 哈萨克族天然绿色食品—奶疙瘩和奶酪[J].新经济, 2015(Z1):148-149.
NURGULI D.The natural green food of Kazak nationality—milk pimple and cheese[J].New Economy, 2015(Z1):148-149.
[2] AZAT R, LIU Y, LI W, et al.Probiotic properties of lactic acid bacteria isolated from traditionally fermented Xinjiang cheese[J].Journal of Zhejiang University Science B, 2016, 17(8):597-609.
[3] 侯宇, 范金波, 张明, 等.乳扇菌群的PCR-DGGE分析及其中乳酸菌的分离与特性研究[J].食品工业科技, 2016, 37(14):181-186.
HOU Y, FAN J B, ZHANG M, et al.PCR-DGGE analysis of lactic acid bacteria and fermentation characteristics of strains isolated from dairy fan[J].Science and Technology of Food Industry, 2016, 37(14):181-186.
[4] 林丽清, 汪欣, 夏九学, 等.内蒙古奶豆腐中优良乳酸菌的筛选及发酵性能研究[J].中国乳品工业, 2018, 46(11):7-10.
LIN L Q, WANG X, XIA J X, et al.Study on the fermentation performances of lactic acid bacteria isolated from traditional fermented milk tofu in Inner Mongolia[J].China Dairy Industry, 2018, 46(11):7-10.
[5] 吴凤玉, 郑喆, 李柳, 等.宫廷奶酪中优良乳酸菌菌株的分离与鉴定[J].食品科学技术学报, 2019, 37(3):55-60;85.
WU F Y, ZHENG Z, LI L, et al.Isolation and identification of lactic acid bacteria from traditional royal cheese[J].Journal of Food Science and Technology, 2019, 37(3):55-60;85.
[6] 热米拉·阿扎提.新疆传统乳制品奶疙瘩中乳酸菌的筛选及其抗衰老活性的研究[D].杭州:浙江大学,2016.
GENMILA A.Study on the selection of lactic acid bacteria in traditionally fermented Xinjiang cheese and analysis of anti-aging effects of selected strains[D].Hangzhou:Zhejiang University, 2016.
[7] 陈欢, 王斌, 史学伟, 等.新疆哈萨克族传统奶酪中潜在益生乳酸菌的筛选[J].中国调味品, 2019, 44(12):50-54.
CHEN H, WANG B, SHI X W, et al.Screening of potential probiotic lactic acid bacteria in Xinjiang Kazak traditional cheese[J].China Condiment, 2019, 44(12):50-54.
[8] ZHENG X J, LIU F, LI K X, et al.Evaluating the microbial ecology and metabolite profile in Kazak artisanal cheeses from Xinjiang, China[J].Food Research International, 2018, 111:130-136.
[9] GUARRASI V, SANNINO C, MOSCHETTI M, et al.The individual contribution of starter and non-starter lactic acid bacteria to the volatile organic compound composition of Caciocavallo Palermitano cheese[J].International Journal of Food Microbiology, 2017, 259:35-42.
[10] GONÇALVES M T P,BENITO M J,CÓRDOBA M D G, et al.Bacterial communities in Serpa cheese by culture dependent techniques, 16S rRNA gene sequencing and high-throughput sequencing analysis[J].Journal of Food Science, 2018, 83(5):1 333-1 341.
[11] ZHU L, ZENG C L, YANG S, et al.Diversity and specificity of the bacterial community in Chinese horse milk cheese[J].MicrobiologyOpen, 2020, 9(8):e1066.
[12] 米瑞芳, 陈曦, 熊苏玥, 等.传统自然发酵酸肉中细菌群落多样性与风味品质分析[J].食品科学, 2019, 40(2):85-92.
MI R F, CHEN X, XIONG S Y, et al.Bacterial community diversity and flavor characteristics of traditional naturally fermented sour meat[J].Food Science, 2019, 40(2):85-92.
[13] 梁晨, 杜海, 徐岩.大曲贮存过程中原核微生物群落结构及风味成分演替规律[J].微生物学通报, 2017, 44(2):384-393.
LIANG C, DU H, XU Y.The succession of procaryotic microbial community and the flavor components in the storage process of Daqu[J].Microbiology, 2017, 44(2):384-393.
[14] LIANG H P, HE Z, WANG X Y, et al.Bacterial profiles and volatile flavor compounds in commercial Suancai with varying salt concentration from Northeastern China[J].Food Research International, 2020, 137:109384.
[15] 尚雪娇, 王玉荣, 杨江, 等.应用Illumina MiSeq高通量测序技术解析梅干菜中细菌多样性[J].中国酿造, 2019, 38(1):140-144.
SHANG X J, WANG Y R, YANG J, et al.Bacterial diversity in preserved vegetable by Illumina MiSeq high throughput sequencing technology[J].China Brewing, 2019, 38(1):140-144.
[16] 郑晓吉, 刘飞, 任全路, 等.基于SPME-GC-MS法比较新疆哈萨克族不同居住区奶酪风味差异[J].食品科学, 2018, 39(8):83-89.
ZHENG X J, LIU F, REN Q L, et al.Comparative analysis of volatile compounds in kazak cheeses from different regions of Xinjiang by SPME-GC-MS[J].Food Science, 2018, 39(8):83-89.
[17] 刘登勇, 周光宏, 徐幸莲.确定食品关键风味化合物的一种新方法:“ROAV”法[J].食品科学, 2008, 29(7):370-374.
LIU D Y, ZHOU G H, XU X L.ROAV method:A new method for determining key odor compounds of Rugao ham[J].Food Science, 2008, 29(7):370-374.
[18] 李露, 王蓓, 王绒雪, 等.奶疙瘩特征风味组分研究[J].中国食品学报, 2018, 18(9):297-302.
LI L, WANG B, WANG R X, et al.Study on characteristic aroma components of kurut[J].Journal of Chinese Institute of Food Science and Technology, 2018, 18(9):297-302.
[19] 孙宝国, 陈海涛.食用调香术[M].第三版.北京:化学工业出版社, 2016.
SUN B G, CHEN H T.Food Flavoring Technique[M].3rd edition.Beijing:Chemical Industry Press, 2016.
[20] 秦晓. 养殖暗纹东方鲀风味物质鉴定分析[D].上海:上海海洋大学, 2015.
QIN X.Characterization of flavor substances of farmed Takifugu obscurus[D].Shanghai:Shanghai Ocean University, 2015.
[21] 穆硕, 刘鑫宇, 罗洁, 等.原料乳体细胞数对硬质干酪蛋白水解及风味与质构品质的影响[J].食品科学, 2019, 40(15):64-70.
MU S, LIU X Y, LUO J, et al.Effect of raw milk somatic cell count on protein hydrolysis and flavor and texture quality of hard cheese[J].Food Science, 2019, 40(15):64-70.
[22] 里奥·范海默特.化合物香味阈值汇编[M].第二版.北京:科学出版社, 2015.
LEO V H.Compilation of Flavor Threshold Values in Water and Other Media[M].2nd edition.Beijing:Science Press, 2015.
[23] GIRI A, OSAKO K, OHSHIMA T.Identification and characterisation of headspace volatiles of fish miso, a Japanese fish meat based fermented paste, with special emphasis on effect of fish species and meat washing[J].Food Chemistry, 2010, 120(2):621-631.
[24] 樊艳. SPME-GC-MS结合ROAV分析腐乳中的主体风味物质[J].食品工业科技, 2021, 42(8):227-234.
FAN Y.Analysis of main flavor substances in fermented soybean curd by SPME-GC-MS and ROAV[J].Science and Technology of Food Industry, 2021, 42(8):227-234.
[25] LIU H, WANG Z Y, ZHANG D Q, et al.Characterization of key aroma compounds in Beijing roasted duck by gas chromatography-olfactometry mass spectrometry, odor activity values, and aroma recombination experiments[J].Journal of Agricultural and Food Chemistry, 2019, 67(20):5 847-5 856.
Outlines

/