Farnesene is a sesquiterpene compound which has a wide range of applications in food, fragrance & flavor and other industries. Farnesene synthase is the key enzyme in biosynthesis of farnesene. Its properties significantly affect the formation of farnesene. A farnesene synthase gene from Citrus sinensis (FSCS) was cloned and expressed in Escherichia coli BL21(DE3). The recombinant enzyme was purified and its biochemical characteristics were investigated. Results showed that FSCS could produce α-farnesene by using farnesyl diphosphate as substrate. In addition, Mg2+ was indispensable, and K+ could enhance the activity of FSCS. However, 8 mmol/L or more Mn2+ significantly inhibited its activity. The optimal condition of Mg2+ and K+ were 30 mmol/L and 40 mmol/L, respectively. The optimal temperature was 20℃, and sensitive to higher temperature. Under the optimal conditions in recombinant Saccharomyces cerevisiae, the yield of farnesene was 43.76 mg/L increased by 30%. The results could provide reference for efficient production of farnesene in microbial cell factories.
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