Food and Fermentation Industries >

2021 , Vol. 47 >Issue 21: 123 - 131

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026594

Effect of lotus leaf flavonoids on liver injury induced by D-galactose in mice

  • LI Chong ,
  • GOU Yuting ,
  • QIAN Yu ,
  • ZHAO Xin
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  • 1(Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China)
    2(Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing 400067, China)
    3(Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing 400067, China)
    4(College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China)

Received date: 2020-12-31

  Revised date: 2021-03-12

  Online published: 2021-11-30

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Abstract

The flavonoids extracted from the lotus leaf were used as the research object. The antioxidant activity of flavonoids was investigated by DPPH and ABTS cation ion free radical scavenging experiments in vitro, and the high antioxidant activity of these extracts was positively correlated with the dose. Forty male Kunming mice were randomly divided into normal group, model group, low dose group and high dose group. The liver injury model was induced by D-galactose, and the model group, low dose group and high dose group were intraperitoneally injected with D-galactose 120 mg/(kg·d). Different doses of lotus leaf flavonoids were used for intervention. Mice in the low-dose and high-dose group were given 250 and 500 mg/(kg·d) lotus leaf flavonoids per day, respectively. The contents of malondialdehyde (MDA), glutathione (GSH), NO, glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were determined by using kit colorimetry. The concentrations of cytokines IL-6, IL-10, TNF-α and IL-1β were determined by ELISA. The organ index and pathological changes were observed. In addition, the variety and content of flavonoids extracted from lotus leaf were determined by HPLC. The results showed that flavonoids had a good scavenging ability of DPPH and ABTS cation ion free radicals, and increased SOD, GSH and GSH-Px content, reduced MDA, NO content significantly. Lotus flavone could significantly improve the liver cell oxidative damage induced by D-galactose in mice. In addition, four bioactive components, rutin, isoquercitrin, astragalin and quercetin, could be detected from lotus leaf by HPLC analysis. In a word, flavonoids extracted from lotus leaf have the good anti-oxidation ability, its pharmacological value can be further studied.

Key words： lotus leaf flavonoids; mice; antioxidant activity; D-galactose; high performance liquid chromatography

Cite this article

LI Chong , GOU Yuting , QIAN Yu , ZHAO Xin . Effect of lotus leaf flavonoids on liver injury induced by D-galactose in mice[J]. Food and Fermentation Industries, 2021 , 47(21) : 123 -131 . DOI: 10.13995/j.cnki.11-1802/ts.026594

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