α-Arbutin is a glycoside, which has good effects on anti-inflammatory, wound repair and whitening. The synthesis of α-arbutin by biotransformation has the characteristic of high efficiency and environmentally friendly. This study used Bacillus subtilis as the host to express the heterologous enzyme, sucrose phosphorylase from Streptococcus mutans (SmSPI336L), as a biocatalyst to convert sucrose and hydroquinone (HQ) into α-arbutin with high efficiency. Firstly, by investigating the protein expression ability, Bacillus subtilis WB800 was determined as a suitable host. The promoter optimization of SmSPI336L and genome integration were performed in B. subtilis, obtaining the recombinant B. subtilis that synthesized α-arbutin by whole-cell catalysis. The yield of α-arbutin was 54.05 g/L, and the molar conversion rate of substrate HQ was 43.7%. Then, by optimizing the ribosome binding site (RBS) sequence of SmSPI336L, the yield of α-arbutin and the molar conversion rate of substrate HQ were significantly improved, reaching to 99.14 g/L and 80.15%, respectively. Finally, the conditions of whole-cell catalysis were optimized. The catalytic volume was expanded to 20 mL and the catalytic time was extended from 22 h to 34 h, which further increased the production of α-arbutin and stabilized the output. The optimized production of α-arbutin reached to 119.44 g/L and the molar conversion rate of substrate HQ was 96.56%. The results of this study are of great significance to the industrial production of α-arbutin.
ZHOU Qi
,
LYU Xueqin
,
CHAI Xueying
,
LIU Yanfeng
,
LI Jianghua
,
DU Guocheng
,
LIU Long
. Highly efficient synthesis of α-arbutin by whole-cell catalysis of Bacillus subtilis[J]. Food and Fermentation Industries, 2021
, 47(22)
: 1
-7
.
DOI: 10.13995/j.cnki.11-1802/ts.027188
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