Yeast spores are efficiently internalized in mammalian cells because the spore wall contains molecules that can induce phagocytosis/micropinocytosis even in nonprofessional phagocytes. In this study, a delivery method of μm sized particles to nonprofessional phagocytes using spore wall materials was built. High salt eluent of spores was either electrostatically attached or crosslinked to latex beads (Φ=2 μm) with surface amino groups. The latex beads were incubated with HEK293T cells to test whether they were internalized in the cells. Spore wall derived molecules can induce uptake of the latex beads even when they were attached to the beads via electrostatic interaction. The uptake efficiency can be improved by crosslinking the spore wall materials to the beads. The results showed that spore wall eluent could induce uptake of μm sized particles. Thus, the spore wall materials could be applied to cell delivery systems. The method would be beneficial particularly to deliver relatively larger particles to nonprofessional phagocytes.
SONG Chaoqun
,
YANG Yan
,
LI Feng
,
LIU Guoyu
,
GAO Xiaodong
,
NAKANISHI Hideki
. Application of Saccharomyces cerevisiae spore wall derived material to deliver micrometer-sized particles to nonprofessional phagocytes HEK293T[J]. Food and Fermentation Industries, 2021
, 47(22)
: 24
-29
.
DOI: 10.13995/j.cnki.11-1802/ts.027074
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