Study on the identification of specialty honey of Apis cerana from the three Gorges Reservoir area of Chongqing based on gas chromatography-ion mobility spectrometry

  • LIU Zhenping ,
  • NIE Qingyu ,
  • PANG Kejing ,
  • ZHANG Yan ,
  • JIANG Rong
Expand
  • 1(Chongqing Vocational Institute of Safety & Technology, Chongqing 404020, China)
    2(Chongqing Three Gorges Vocational College, Chongqing 404155, China)

Received date: 2021-03-01

  Revised date: 2021-03-22

  Online published: 2021-12-16

Abstract

The volatile organic compounds of four different plant sources honey of Apis cerana, rape flower honey, gallnut flower honey, loquat flower honey and orange blossom honey in the three Gorges Reservoir area of Chongqing, were determined and analyzed by gas chromatography-ion mobility spectrometry (GC-IMS). The discriminant model to identify and classify different plant sources honey was established. Two-dimensional differential analysis was used to screen out 58 effective characteristic peaks for the characterization of the different plant sources of honey of Apis cerana. A discriminant model was established by principal component analysis (PCA) and linear discriminant analysis (LDA). The results showed that the cumulative contribution rate of the top three principal components in this study was 81.35%, and the four kinds of honey of Apis cerana in the scores plot were distributed in different areas without overlapping or crossing. The established discriminant model could effectively identify the honey of Apis cerana from different plant sources, and the recognition accuracy was 100%. The misdiagnosis rate for honey of Apis cerana from other plant sources was only 5.9%. In this study, volatile components in honey of Apis cerana were determined by GC-IMS, the honey of Apis cerana samples of different plant sources in the three Gorges Reservoir area of Chongqing was accurately distinguished through PCA and LDA analysis, providing a new technical reference for the variety identification and quality control of honey of Apis cerana.

Cite this article

LIU Zhenping , NIE Qingyu , PANG Kejing , ZHANG Yan , JIANG Rong . Study on the identification of specialty honey of Apis cerana from the three Gorges Reservoir area of Chongqing based on gas chromatography-ion mobility spectrometry[J]. Food and Fermentation Industries, 2021 , 47(22) : 273 -278 . DOI: 10.13995/j.cnki.11-1802/ts.027151

References

[1] TETTE P A S, GUIDI L R, BASTOS E M A F, et al.Synephrine - A potential biomarker for orange honey authenticity[J].Food Chemistry, 2017, 229:527-533.
[2] ALQARNI A S, OWAYSS A A, MAHMOUD A A, et al.Mineral content and physical properties of local and imported honeys in Saudi Arabia[J].Journal of Saudi Chemical Society, 2014, 18(5):618-625.
[3] BLASCO C, VAZQUEZ-ROIG P, ONGHENA M, et al.Analysis of insecticides in honey by liquid chromatography-ion trap-mass spectrometry:comparison of different extraction procedures[J].Journal of Chromatography A, 2011, 1218(30):4 892-4 901.
[4] SILVA L R, LIMA S M P, VASCONCELOS N A E L M.Cultural diversity- dialogue between interdisciplinary indigenous peoples and non indigenous - an experiment in initial teacher training[J].Revista Ambivalências, 2016, 4(7):309-323.
[5] FINOLA M S, LASAGNO M C, MARIOLI J M.Microbiological and chemical characterization of honeys from central Argentina[J].Food Chemistry, 2007, 99(4):1 649-1 653.
[6] DOWNEY G, HUSSEY K, KELLY J D, et al.Preliminary contribution to the characterisation of artisanal honey produced on the island of Ireland by palynological and physico-chemical data[J].Food Chemistry, 2005, 91(2):347-354.
[7] KOMATSU S S, MARCHINI L C, DE C C MORETIA A C.Análises físico-químicas de amostras de méis de flores silvestres, de eucalipto e de laranjeira, produzidos por Apis mellifera L., 1758 (Hymenoptera, Apidae) no estado de são paulo.2.conteúdo de açúcares e de proteína[J].Ciência E Tecnologia De Alimentos, 2002, 22(2):143-146.
[8] BASTOS D H M, FRANCO M R B, SILVA M A A P D, et al.Volatile composition and aroma and flavor profiles of eucalyptus and orange honeys[J].Food Technol, 2002, 22(2):122-129.
[9] 曹炜, 卢珂, 陈卫军, 等.不同种类蜂蜜抗氧化活性的研究[J].食品科学, 2005, 26(8):352-356.
CAO W, LU K, CHEN W J, et al.Study on antioxidation effects of different honeys[J].Food Science, 2005, 26(8):223-227.
[10] FALLICO B, ZAPPALÀ M, ARENA E, et al.Effects of conditioning on HMF content in unifloral honeys[J].Food Chemistry, 2004, 85(2):305-313.
[11] 杨娟. 基于多种光谱技术的蜂蜜和蜂胶品种鉴别研究[D].北京:中国农业科学院,2016.
YANG J.Identification of honey and Propolis varieties based on various spectral techniques[D].Beijing:Chinese Academy of Agricultural Sciences, 2016.
[12] 张颖, 张光艳, 王宇翔, 等.不同花源蜂蜜蛋白质组分及提取方法的比较[J].食品与发酵工业, 2019, 45(14):91-96.
ZHANG Y, ZHANG G Y, WANG Y X, et al.Comparison of protein composition and extraction methods between honey from different floral origins[J].Food and Fermentation Industries, 2019, 45(14):91-96.
[13] 程妮. 蜂蜜植物源鉴别新方法及其保肝活性研究[D].西安:西北大学, 2015.
CHENG N.The novel method for identification of the botanic origin of honey and its hepatoprotection[D].Xi'an:Northwest University, 2015.
[14] CAVAZZA A, CORRADINI C, MUSCI M, et al.High-performance liquid chromatographic phenolic compound fingerprint for authenticity assessment of honey[J].Journal of the Science of Food and Agriculture, 2013, 93(5):1 169-1 175.
[15] 贺琼, 何亮亮, 康予馨, 等.高效液相色谱-电化学检测指纹图谱鉴别3种单花种蜂蜜花源[J].食品科学, 2017, 38(2):290-295.
HE Q, HE L L, KANG Y X, et al.Authentication of three monofloral honeys by high performance liquid chromatography with electrochemical detection[J].Food Science, 2017, 38(2):290-295.
[16] KARABAGIAS I K, BADEKA A, KONTAKOS S, et al.Characterization and classification of Thymus capitatus (L.) honey according to geographical origin based on volatile compounds, physicochemical parameters and chemometrics[J].Food Research International, 2014, 55:363-372.
[17] ROBOTTI E, CAMPO E, RIVIELLO M, et al.Optimization of the extraction of the volatile fraction from honey samples by SPME-GC-MS, experimental design, and multivariate target functions[J].Journal of Chemistry, 2017:1-14.
[18] JANDRIC' Z, HAUGHEY S A, FREW R D, et al.Discrimination of honey of different floral origins by a combination of various chemical parameters[J].Food Chemistry, 2015, 189:52-59.
[19] 孙涛, 夏明星, 孔德英, 等.五倍子蜂蜜TaqMan探针实时荧光定量PCR检测方法的建立[J].贵州农业科学, 2016, 44(8):15-18.
SUN T, XIA M X, KONG D Y, et al.Establishment of a TaqMan real-time PCR assay for detecting the Chinese gall honey[J].Guizhou Agricultural Sciences, 2016, 44(8):15-18.
[20] GOK S, SEVERCAN M, GOORMAGHTIGH E, et al.Differentiation of Anatolian honey samples from different botanical origins by ATR-FTIR spectroscopy using multivariate analysis[J].Food Chemistry, 2015, 170:234-240.
[21] GERHARDT N, BIRKENMEIER M, SCHWOLOW S, et al.Volatile-compound fingerprinting by headspace-gas-chromatography ion-mobility spectrometry (HS-GC-IMS) as a benchtop alternative to 1H NMR profiling for assessment of the authenticity of honey[J].Analytical Chemistry, 2018, 90(3):1 777-1 785.
[22] HERNÁNDEZ-MESA M, ESCOURROU A, MONTEAU F, et al.Current applications and perspectives of ion mobility spectrometry to answer chemical food safety issues[J].TrAC Trends in Analytical Chemistry, 2017, 94:39-53.
[23] VAUTZ W, FRANZKE J, ZAMPOLLI S, et al.On the potential of ion mobility spectrometry coupled to GC pre-separation-A tutorial[J].Analytica Chimica Acta, 2018, 1 024:52-64.
[24] KARPAS Z.Applications of ion mobility spectrometry (IMS) in the field of foodomics[J].Food Research International, 2013, 54(1):1 146-1 151.
[25] 王卫国, 梁茜茜, 程沙沙, 等.离子迁移谱检测痕量爆炸物新技术和应用[J].科学通报, 2014, 59(12):1 079-1 086.
WANG W G, LIANG X X, CHENG S S, et al.Development of ion mobility spectrometry and its application for detection trace explosives[J].Chinese Science Bulletin, 2014, 59(12):1 079-1 086.
[26] VAUTZ W, SLODZYNSKI R, HARIHARAN C, et al.Detection of metabolites of trapped humans using ion mobility spectrometry coupled with gas chromatography[J].Analytical Chemistry, 2013, 85(4):2 135-2 142.
[27] 冯雪, 尹利辉, 金少鸿, 等.离子迁移谱及其在药学领域的应用[J].药物分析杂志, 2013, 33(7):1 109-1 114.
FENG X, YIN L H, JIN S H, et al.Ion mobility spectrometry and its applications in pharmaceutical research and development[J].Chinese Journal of Pharmaceutical Analysis, 2013, 33(7):1 109-1 114.
[28] HOLLINGSWORTH B V, REICHENBACH S E, TAO Q, et al.Comparative visualization for comprehensive two-dimensional gas chromatography[J].Journal of Chromatography A, 2006, 1 105(1-2):51-58.
[29] CAVANNA D, ZANARDI S, DALL'ASTA C, et al.Ion mobility spectrometry coupled to gas chromatography:A rapid tool to assess eggs freshness[J].Food Chemistry, 2018, 271:691-696.
[30] 苏学素, 张晓焱, 焦必宁, 等.基于近红外光谱的脐橙产地溯源研究[J].农业工程学报, 2012, 28(15):240-245.
SU X S, ZHANG X Y, JIAO B N, et al.Determination of geographical origin of navel orange by near infrared spectroscopy[J].Transactions of the Chinese Society of Agricultural Engineering, 2012, 28(15):240-245.
Outlines

/