Two enzyme transformation technologies for trehalose production

  • CAO Yuan ,
  • LI Ying
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  • (Nanjing KaiTong Grain Biochemical R&D Co.Ltd., Nanjing 210012, China)

Received date: 2021-02-20

  Revised date: 2021-03-29

  Online published: 2021-12-16

Abstract

As a non-reducing sugar with special biological protection function, trehalose is widely used in food, medicine and other industries. Enzyme conversion technology represented by double-enzyme method and single-enzyme method is the current hotspot. Double-enzyme method is a technology that takes starch as raw material and converts amylose with a certain chain length into trehalose under the synergistic action of maltooligosyl trahalose synthase (MTSase) and maltooligosyl trahalose trahalohydrolase (MTHase). Double-enzyme method was originated in Japan with the advantages of low-cost raw materials, maturity technology and higher conversion rate. In single enzyme method, maltose directly converted to trehalose by trehalose synthase (TreS) has attracted wide attention because its reaction process is easily controlled, with simple process flow and less by-products. With the development of protein genetic engineering, the optimization construction technology of biological enzyme is becoming more mature. It lays the foundation for the further improvement of double-enzyme method and industrial application of single-enzyme method.

Cite this article

CAO Yuan , LI Ying . Two enzyme transformation technologies for trehalose production[J]. Food and Fermentation Industries, 2021 , 47(22) : 319 -325 . DOI: 10.13995/j.cnki.11-1802/ts.027098

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