In order to investigate the effect on polysaccharide synthesis by overexpression of glucosyltransferase GL24971, the overexpression plasmid was transformed into G. lucidum protoplast. The results showed that the maximum production of extracellular polysaccharide in T1 and T2 was 0.07 and 0.06 mg/mg, which increased by 132.4% and 70.0%, respectively. The yield of intracellular polysaccharide of T1 and T2 were both 0.03 mg/mg, which decreased by 10.7% and 37.4%, respectively. Secondly, the content of chitin and β-1, 3-glucan decreased. The results of the monosaccharide composition analysis showed that the proportion of galactose was decreased with the increased mannose proportion, in which glucose was still the main component. These results provide new ideas for the enhancement of G. lucidum polysaccharide production and further clarify the polysaccharide synthesis pathway.
CHEN Yun
,
ZHAO Liting
,
GU Zhenghua
,
LI Youran
,
SHI Guiyang
,
DING Zhongyang
. Effect of glycosyltransferase GL24971 on polysaccharide synthesis in Ganoderma lucidum[J]. Food and Fermentation Industries, 2021
, 47(23)
: 1
-7
.
DOI: 10.13995/j.cnki.11-1802/ts.027324
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