Food and Fermentation Industries >

2021 , Vol. 47 >Issue 23: 16 - 23

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026923

Efficiently harvesting heterotrophic Chlorella vulgaris through co-culture and mixed mycelium of Aspergillus niger

  • ZHENG Xiao ,
  • ZHOU Hualan ,
  • GULTOM Sarman Oktovianus ,
  • DENG Jiabao ,
  • ZHANG Jianguo
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  • 1(Institute of Food Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)
    2(Department of Agricultural and Biosystems Engineering,Papua University,Manokwari,Papua Barat,Indonesia)

Received date: 2021-01-29

  Revised date: 2021-03-30

  Online published: 2021-12-31

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Abstract

The process of microalgae harvest was difficult and cost because of its small size and low cell density. Aspergillus niger ATCC 1015 co-cultured with Chlorella vulgaris FACHB-8 was optimized to harvest microalgae efficiently. The optimal harvest conditions were as following: 130 r/min 0-24 h followed by 150 r/min for 24-48 h, the initial pH 4.5, 0.02 g/L of FeSO4, 0.5 g/L of MgSO4, 10 g/L of glucose for 0-24 h, 8 g/L of glucose for 24-48 h, 5×107 spores /mL of initial spore concentration, OD680 of initial microalgae concentration 0.80, and culturing for 2 d. Under these conditions, the maximum harvesting rate of Chlorella cells was 88.79%. Therefore, C. vulgaris FACHB-8 cells were collected by A. niger efficiently at heterotrophic condition, which provides a basis for the wide application of Chlorella in food industry.

Key words： Aspergillus niger; Chlorella vulgaris; spore concentration; culture conditions; harvesting ratio

Cite this article

ZHENG Xiao , ZHOU Hualan , GULTOM Sarman Oktovianus , DENG Jiabao , ZHANG Jianguo . Efficiently harvesting heterotrophic Chlorella vulgaris through co-culture and mixed mycelium of Aspergillus niger[J]. Food and Fermentation Industries, 2021 , 47(23) : 16 -23 . DOI: 10.13995/j.cnki.11-1802/ts.026923

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