In order to increase the yield of adenosine produced by fermentation and solve the problems of short adenosine production cycle and low efficiency of converting glucose into adenosine in the existing method, this paper uses Bacillus subtilis XGL (8-AGr+ His-+xan-+SGr) as the test strains to study the production of adenosine by membrane dialysis fermentation. Adenosine was fermented in a 5 L fermentor, and the fermentation broth was subjected to membrane-coupled dialysis twice at 36 h and 60 h. The fermentation cycle was extended to 96 h, with the OD600nm of the bacteria increased to 94.6, and produce 448.3 g adenosine in a single batch. The average adenosine production was 44.2 g/L. The rate of glucose conversion to adenosine increased to 25.1%. Compared with the traditional fermentation process, membrane-coupled batch dialysis fermentation prolonged the adenosine production cycle by 36-40 h, increased the biomass of bacteria by 29.6%, increased the yield of a single batch by 298.4 g, and increased the conversion rate of glucose to adenosine by 8.8%. Through membrane dialysis, the production of by-product acetoin was reduced, and more carbon sources flowed to adenosine synthesis pathway. The activity of amidophosphoribosyl transferase and adenylosuccinate synthase in the dialysis fermentation was detected at 52 h of fermentation compared with the normal fermentation process. Therefore, the production of adenosine by fermentation coupled with membrane dialysis can effectively relieve the feedback inhibition of the products in the fermentation broth, reduce the mass concentration of by-products, extend the fermentation cycle, and effectively increase the biomass of bacteria in the fermentation broth, adenosine production and adenosine conversion rate.
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