Food and Fermentation Industries >

2021 , Vol. 47 >Issue 23: 83 - 90

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027300

Effects of antifungal peptide P-1 from Bacillus pumilus HN-10 on cell membrane structure and ROS metabolism of Trichothecium roseum

  • WU Shujuan ,
  • YUN Jianmin ,
  • WANG Rui ,
  • HE Kui ,
  • MAO Yongqiang ,
  • ZHAO Fengyun ,
  • ZHANG Wenwei ,
  • AI Duiyuan
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  • (College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070,China)

Received date: 2021-03-11

  Revised date: 2021-04-07

  Online published: 2021-12-31

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Abstract

This study explored the effects of antifungal peptide P-1 from Bacillus pumilus HN-10 on cell membrane structure and reactive oxygen species (ROS) metabolism of T. roseum. The inhibitory effect of antifungal P-1 on the growth of T. roseum was investigated by plate cultivation (Oxford cup method) and liquid cultivation system, and the effect of antifungal P-l on the cell membrane structure of T. roseum in liquid cultivation within 12 h was dynamically observed by propidiumiodide and transmission electron microscope. The influence of antifungal P-1 on membrane permeability was analyzed by liquid cultivation system, and based on membrane lipid peroxidation, the effect of antifungal P-l on ROS and its metabolite malondialdehyde (MDA) was studied. The results showed that the growth of T. roseum spores was obviously inhibited by solid and liquid cultivation. With the extension of treatment time of antifungal peptide P-1, the number of T. roseum spores increased gradually. The treatment of antifungal peptide P-1 caused local damage to the spore wall and membrane structure of T. roseum, appeared some gaps and lead to massive outflow of cytoplasm and deformation of spores. The treatment of antifungal P-1 treatment reduced the ergosterol content of the mycelium, and it was 35% lower than the control group on 7th day. At the same time, the treatment of antifungal P-1 treatment significantly increased the uptake rate of N-phenyl-1-naphthylamine, relative conductivity. It was 24%, 35% higher than that of the control group on 7th day, respectively. In addition, intracellular ·O-2 was significantly increased by P-1 treatment (P<0.05), disrupted the balance of cellular antioxidant (SOD, CAT, POD) systems, aggravated lipid peroxidation and protein carbonylation, increased MDA content,and finally inhibited mycelium growth.

Key words： antifungal peptide P-1 from Bacillus pumilus HN-10; Trichothecium roseum; cell membrane; reactive oxygen species (ROS)

Cite this article

WU Shujuan , YUN Jianmin , WANG Rui , HE Kui , MAO Yongqiang , ZHAO Fengyun , ZHANG Wenwei , AI Duiyuan . Effects of antifungal peptide P-1 from Bacillus pumilus HN-10 on cell membrane structure and ROS metabolism of Trichothecium roseum[J]. Food and Fermentation Industries, 2021 , 47(23) : 83 -90 . DOI: 10.13995/j.cnki.11-1802/ts.027300

References

[1] 郑鄢燕,赵力卉,王宇滨,等.链格孢、粉红单端孢通过降解果皮细胞壁导致哈密瓜病害[J].食品与发酵工业,2020,46(10):124-131.
ZHENG Y Y,ZHAO L H,WANG Y B,et al.Alternaria alternata and Trichothecium roseum cause disease of Hami melon by degrading the cell wall in the pericarp[J].Food and Fermentation Industries,2020,46(10):124-131.
[2] TANG Y M,XUE H L,BI Y,et al.A method of analysis for T-2 toxin and neosolaniol by UPLC-MS/MS in apple fruit inoculated with Trichothecium roseum[J].Food Additives and Contaminants,2015,32(4):480-487.
[3] 赵冠华,牛黎莉,罗荣涛,等.3种抗氧化剂对硅酸钠胁迫下粉红单端孢(Trichothecium roseum)孢子生存能力的保护作用比较研究[J].食品科学,2015,36(17):191-195.
ZHAO G H,NIU L L LUO R T,et al.Comparison of protective effects of three antioxidants on spore viability of Trichothecium roseum under sodium silicate stress[J].Food science,2015,36(17):191-195.
[4] VALLEJO-REYNA M A,SANTAMARÍA J M,RODRÍGUEZ-ZAPATA L C,et al.Identification of novel ERF transcription factor genes in papaya and analysis of their expression in different tissues and in response to the plant defense inducer benzothiadiazole(BTH)[J].Physiological & Molecular Plant Pathology,2015,91(1):141-151.
[5] 白小东,牛黎莉,毕阳,等.18种盐对苹果霉心病的控制及部分机理[J].食品工业科技,2015,36(10):335-338.
BAI X D,NIU L L,BI Y,et al.18 kinds of salt on apple of core rot control and part of the mechanism[J].Science and Technology of Food Industry,2015,36(10):335-338.
[6] 王为栋,孙海娟.抗菌肽生物活性及其作用机理研究进展[J].当代畜牧,2018(33):32-34.
WANG W D,SUN H J.Research progress on antimicrobial peptides bioactivity and its mechanism[J].Contemporary Animal Husbandry,2018(33):32-34.
[7] 王亚平,余维维,秦梦茹,等.抗菌肽的作用机理及应用[J].湖北农业科学,2018,57(5):9-13;70.
WANG Y P,YU W W,QIN M R,et al.Antibacterial mechanism and application of antimicrobial peptides[J].Hubei Agricultural Sciences,2018,57(5):9-13;70.
[8] 黄玉琴,贠建民,张紊玮,等.Bacillus pumilus HN-10对Trichothecium roseum的拮抗作用及菌体结构的影响[J].食品与生物技术学报,2019,38(6):25-33.
HUANG Y Q,YUN J M,ZHANG W W,et al.Effect of Bacillus pumilus HN-10 on antagonistic activity and cell structure of Trichothecium roseum[J].Journal of Food Science and Biotechnology,2019,38(6):25-33.
[9] 严海娇,贠建民,白杰,等.短小芽孢杆菌HN-10抗菌肽的分离纯化及其抗粉红单端孢活性[J].食品科学,2018,39(22):123-128.
YAN H J,YUN J M,BAI J,et al.Purification of an antimicrobial peptide from Bacillus pumilus HN-10 and its inhibitory activity against Trichothecium roseum[J].Food science,2018,39(22):123-128.
[10] YAN H J,YUN J M,AI D Y,et al.Two novel cationic antifungal peptides isolated from Bacillus pumilus HN-10 and their inhibitory activity against Trichothecium roseum[J].World Journal of Microbiology and Biotechnology,2018,34(2):1-10.
[11] 郭娟,贠建民,邓展瑞,等.Bacillus pumilus HN-10抗菌肽P-1对粉红单端孢的抑菌机理[J].食品科学,2019,40(19):17-22.
GUO J,YUN J M,DENG Z R,et al.Antimicrobial mechanism of antimicrobial peptide P-1 from Bacillus pumilus HN-10 against Trichothecium roseum[J].Food Science,2019,40(19):17-22.
[12] 刘志恬,董玉鹏,李永才,等.ε-聚赖氨酸对梨果实黑斑病菌Alternaria alternata的抑制作用及其机理[J].食品科学,2021,42(11):213-220.
LIU Z T,DONG Y P,LI Y C,et al.Antifungal effect of ε-polylysine on Alternaria alternata isolated from pears with black spot and its possible mechanism[J].Food Science,2021,42(11):213-220.
[13] WANG L T,WU H,QIN G Z,et al.Chitosan disrupts Penicillium expansum and controls postharvest blue mold of jujube fruit[J].Food Control,2014,41:56-62.
[14] HELAL G A,SARHAN M M,ABU SHAHLA A N K,et al.Effects of Cymbopogon citratus L.essential oil on the growth,morphogenesis and aflatoxin production of Aspergillus flavus ML2-strain[J].Journal of Basic Microbiology,2007,47(1):5-15.
[15] LIU Y,ZHU X Q,LI W D,et al.Enhancing production of ergosterol in Pichia pastoris GS115 by over-expression of 3-hydroxy-3-methylglutaryl CoA reductase from Glycyrrhiza uralensis[J].Acta Pharmaceutica Sinica B,2014,4(2):161-166.
[16] 付永岩,李盈盈,姜瞻梅,等.牛乳铁蛋白衍生肽FP-PF的安全性及其抑菌机理[J].中国食品学报,2020,20(8):92-99.
FU Y Y,LI Y Y,JIANG Z M,et al.Safety and antibacterial mechanism of bovine lactoferrin derived peptide FP-PF[J].Journal of Chinese Institute of Food Science and Technology,2020,20(8):92-99.
[17] 王雪燕,陈瑛,张嘉敏,等.草鱼鱼鳞抗菌肽与肉桂精油联合抑菌作用及机理[J].食品科学,2020,41(23):100-106.
WANG X Y,CHEN Y,ZHANG J M,et al.Combined antimicrobial effect and mechanism of antimicrobial peptide from grass carp scale and cinnamon essential oil[J].Food Science,2020,41(23):100-106.
[18] PUSHPANATHAN M,GUNASEKARAN P,RAJENDHRAN J.Mechanisms of the antifungal action of marine metagenome-derived peptide,MMGP1,against Candida albicans[J].PLoS One,2013,8(7):e69316.
[19] 段丽菊,刘英帅,朱燕,等.DNPH比色法:一种简单的蛋白质羰基含量测定方法[J].毒理学杂志,2005,19(4):320-322.
DUAN L J,LIU Y S,ZHU Y,et al.DNPH colorimetry:A simple method for the determination of protein carbonyl content[J].Journal of Toxicology,2005,19(4):320-322.
[20] 王霆,张雨,刘宏,等.臭氧熏蒸处理联合PE包装对金针菇采后贮藏品质及抗氧化能力的影响[J].中国农业科学,2020,53(4):823-835.
WANG T,ZHANG Y,LIU H,et al.Effects of ozone fumigation combined with PE packaging on postharvest storage quality and antioxidant capacity of Flammulina velutipes[J].Scientia Agricultura Sinica,2020,53(4):823-835.
[21] 王新绘,孙九丽,苟萍.灰葡萄孢菌AUR1基因内含子对肌醇磷脂酰神经酰胺合成酶表达的影响及致病性[J].微生物学报,2014,54(11):1 304-1 310.
WANG X H,SUN J L,GOU P.Effect of AUR1 gene intron on the expression of inositol phosphorylceramide synthase and pathogenicity in Botrytis cinerea[J].Acta Microbiologica Sinica,2014,54(11):1 304-1 310.
[22] 燕璐.体外条件下柠檬酸处理对T.roseum的抑制及部分机理[D].兰州:甘肃农业大学,2015.
YAN L.Citric acid inhibits against Trichothecium roseum in vitro and its partial mechanism[D].Lanzhou:Gansu Agricultural University,2015.
[23] 谢栋,彭憬,王津红,等.枯草芽孢杆菌抗菌蛋白X998-III的纯化与性质[J].微生物学报,1998,38(1):13-19.
XIE D,PENG J,WANG J H,et al.Purification and properties of antifungal protein X998-III from Bacillus subtilis[J].Acta Microbiologica Sinica,1998,38(1):13-19.
[24] ALIZADEH F,KHODAVANDI A,ZALAKIAN S.Quantitation of ergosterol content and gene expression profile of ERG11 gene in fluconazole-resistant Candida albicans[J].Current Medical Mycology,2017,3(1):13-19.
[25] HU C C,ZHOU M,WANG W Z,et al.Abnormal ergosterol biosynthesis activates transcriptional responses to antifungal azoles[J].Frontiers in Microbiology,2018,9:9.
[26] MA Q Q,DONG N,SHAN A S,et al.Biochemical property and membrane-peptide interactions of de novo antimicrobial peptides designed by helix-forming units[J].Amino Acids,2012,43(6):2 527-2 536.
[27] 陈惠,赵鑫荟,艾启俊.3种中草药对沙门氏菌的抑制机理初探[J].安徽农业科学,2013,41(25):10 275-10 277.
CHEN H,ZHAO X H,AI Q J.Study on inhibitory mechanism of three Chinese herbals against Salmonella[J].Journal of Anhui Agricultural Sciences,2013,41(25):10 275-10 277.
[28] TSIKAS D.Assessment of lipid peroxidation by measuring malondialdehyde(MDA) and relatives in biological samples:Analytical and biological challenges[J].Analytical Biochemistry,2017,524:13-30.
[29] SHREAZ S,WANI W A,BEHBEHANI J M,et al.Cinnamaldehyde and its derivatives,a novel class of antifungal agents[J].Fitoterapia,2016,112:116-131.
[30] LI Y,ZHANG W,NIU J F,et al.Mechanism of photogenerated reactive oxygen species and correlation with the antibacterial properties of engineered metal-oxide nanoparticles[J].ACS Nano,2012,6(6):5 164-5 173.
[31] YANG Q,WANG J,ZHANG P,et al.In vitro and in vivo antifungal activity and preliminary mechanism of cembratrien-diols against Botrytis cinerea[J].Industrial Crops and Products,2020,154:112745.
[32] SIMAS D L R,DE AMORIM S H B M,GOULART F R V,et al.Citrus species essential oils and their components can inhibit or stimulate fungal growth in fruit[J].Industrial Crops and Products,2017,98:108-115.
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