Food and Fermentation Industries >

2021 , Vol. 47 >Issue 23: 98 - 105

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028407

Structural changes of polysaccharide from Chuanminshen violaceum and its effects on digestive enzyme activities during simulated digestion in vitro

  • GAO Tao ,
  • LUO Huangyang ,
  • WU Ren ,
  • YAN Rui ,
  • HE Lingzhi ,
  • CHENG Fei ,
  • TANG Huali
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  • 1(College of Biology and Food Engineering,Chongqing Three Gorges University,Chongqing 404100,China)
    2(Chongqing Wanzhou Institute for Drug Control,Chongqing 404100,China)

Received date: 2021-06-18

  Revised date: 2021-08-05

  Online published: 2021-12-31

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Abstract

The crude polysaccharides of Chuanminshen violaceum (CVPs) were extracted by ultrasonic-assisted water extraction and alcohol precipitation, and purified using DEAE Cellulose-52 and Sephadex G-100 to obtain the main fractions of CVPs (CVPs-1-G). The structural changes and oligosaccharide production of CVPs-1-G during simulated digestion were investigated by simulation experiment in vitro combined with gel penetration chromatography and mass spectrometry, and the effects of digestion products on the activities of digestive enzymes were determined. In addition, molecular docking technique was used to investigate the effects of oligosaccharides generated after CVPs-1-G digestion on the activities of digestive enzymes. The results showed that CVPs-1-G could be degraded during saliva digestion and intestinal fluid digestion, and was relatively stable during gastric digestion. After simulated digestion, CVPs-1-G mainly produced monosaccharides, disaccharides, trisaccharides, tetrasaccharides and pentasaccharides, in which the content of disaccharides was the highest. Additionally, the inhibitory activities of α-amylase, lipase and pepsin were enhanced after simulated digestion of CVPs-1-G, and the inhibitory activities of digestive enzymes were significantly correlated with the molecular weight and reducing sugar. Furthermore, the results of molecular docking shown that the disaccharide produced by CVPs-1-G after simulated digestion had strong binding ability with α-amylase, lipase and pepsin.

Key words： Chuanminshen violaceum; polysaccharide; glucan; simulated digestion; molecular docking

Cite this article

GAO Tao , LUO Huangyang , WU Ren , YAN Rui , HE Lingzhi , CHENG Fei , TANG Huali . Structural changes of polysaccharide from Chuanminshen violaceum and its effects on digestive enzyme activities during simulated digestion in vitro[J]. Food and Fermentation Industries, 2021 , 47(23) : 98 -105 . DOI: 10.13995/j.cnki.11-1802/ts.028407

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