Food and Fermentation Industries >

2021 , Vol. 47 >Issue 23: 258 - 264

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027213

Research of synchronizating rapid determination of tadalafil and sildenafil in health caring foods

  • OU Aifen ,
  • LUO Yiming ,
  • ZHAO Suqing
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  • 1(School of Biomedical and Pharmaceutical Science,Guangdong University of Technology,Guangzhou 511400,China)
    2(Guangzhou City Polytechnic,Guangzhou 510405,China)
    3(Guangzhou Ruisen Biotechnology Co.Ltd,Guangzhou 511400,China)

Received date: 2021-03-15

  Revised date: 2021-04-20

  Online published: 2021-12-31

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Abstract

Colloidal gold-based immunochromatography was used to establish a combined rapid test card for simultaneous determination of tadalafil and sildenafil in health caring products. The monoclonal antibodies of tadalafil and sildenafil were labeled with colloidal gold and sprayed onto the glass fiber. Tadalafil, sildenafil-coupled OVA antigen and goat anti-rat IgG were respectively scribed onto the nitrocellulose membrane as test line 1, test line 2 and control line. Then, the sample pad, colloidal gold pad, nitrocellulose membrane and absorbent pad were assembled and cut into the test strip. The optimal volume was 100-120 μL and the sensitivity of the rapid detection was 0.2 μg/mL for tadalafil and sildenafil. The false-positive rate and false-negative rate were zero. The technique can be used to detect tadalafil and sildenafil simultaneously, improve the detection speed and accuracy, and reduce the detection cost. It can be used as supervision of the production process and circulation process of health care products and screening of large quantities of samples.

Key words： tadalafil; sildenafil; colloidal gold immunochromatography; synchronizating rapid determination

Cite this article

OU Aifen , LUO Yiming , ZHAO Suqing . Research of synchronizating rapid determination of tadalafil and sildenafil in health caring foods[J]. Food and Fermentation Industries, 2021 , 47(23) : 258 -264 . DOI: 10.13995/j.cnki.11-1802/ts.027213

References

[1] 吴胜男,孙晓红,周艳,等.贵州省两城市居民保健食品知信行调查分析[J].现代预防医学,2017,44(7):1 191-1 193;1 197.
WU S N,SHUN X H,ZHOU Y,et al.Knowledge-Attitude-Practice of health food among residents in two cities of Guizhou[J].Modern Preventive Medicine,2017,44(7):1 191-1 193;1 197.
[2] 巩萍.营养相关慢性病人保健食品认知度和消费现状研究[D].济南:山东大学,2014.
GONG P.The study of congnition and consumption on health foods about patients with chronic disease related nutrition[D].Jinan:Shandong University,2014.
[3] 王浩,陈立功.中药在欧美国家注册管理概况[J].天津药学,2007,19(2):44-45;71.
WANG H,CHEN L G.Introduction of Chinese traditional medicine registration in EU and USA[J].Tianjin Pharmacy,2007,19(2):44-45;71.
[4] ROCHA T,AMARAL J S,OLIVEIRA M B P P.Adulteration of dietary supplements by the illegal addition of synthetic drugs:A review[J].Comprehensive Reviews in Food Science and Food Safety,2016,15(1):43-62.
[5] 曲晓宇,董雷,张四喜,等.HPLC-MS/MS法同时检测抗疲劳药物及保健品中非法添加的7种壮阳类化学药物[J].中国药房,2013,24(33):3 129-3 131.
QU X Y,DONG L,ZHANG S X,et al.Simultaneous detection of 7 illegal added anti-impotence medicines in anti-fatigue preparations and health care products by HPLC-MS/MS[J].China Pharmacy,2013,24(33):3 129-3 131.
[6] 龙凌云,李涛,邓永琪,等.HPLC-MS/MS法同时检测抗疲劳保健食品中非法添加的8种壮阳类化合物[J].农产品加工,2015(12):45-47.
LONG L Y,LI T,DENG Y Q,et al.Simultaneous detection of 8 illegal added anti-impotence medicines in anti-fatigue health care products by HPLC-MS/MS[J].Farm Products Processing,2015(12):45-47.
[7] 倪赞,张崇生.UPLC-MS/MS法测定补肾抗疲劳中成药及保健食品中非法添加的9种PDE-5抑制剂及其衍生物[J].中南药学,2018,16(9):1 295-1 299.
NI Z,ZHANG C S.Determination of 9 phosphodiesterase-5 inhibitors and its derivative illegally added to invigorant herbal medicines and dietary supplements by UPLC-MS/MS[J].Central South Pharmacy.September,2018,16(9):1 295-1 299.
[8] 徐硕,金鹏飞,徐文峰,等.补肾壮阳类药品中非法添加化学药物检测技术的研究进展[J].西北药学杂志,2017,32(5):677-680.
XU S,JIN P F,XU W F,et al.Research advances on analytical technologies of illegally adulterated chemical substances in medicines for invigorants[J].Northwest Pharmaceutical Journal,2017,32(5):677-680.
[9] ULLOA J,SAMBROTTA L,REDKO F,et al.Detection of a tadalafil analogue as an adulterant in a dietary supplement for erectile dysfunction[J].The Journal of Sexual Medicine,2015,12(1):152-157.
[10] 刘子雄,黄景辉,谭贵良,等.超高效液相色谱-串联质谱法测定保健食品中78种非法添加化学药物[J].现代食品科技,2020,36(12):283-292.
LIU Z X,HUANG J H,TAN G L,et al.Determination of 78 chemical drugs illegally added in health food by ultra-high performance liquid chromatography-tandem mass spectrometry[J].Modern Food Science and Technology,2020,36(12):283-292.
[11] 黄朝辉,蔡丹丹,陈仲益,等.保健食品中他达拉非类似物去甲他达拉非的检测研究[J].中国药学杂志,2015,50(4):371-374.
HUANG Z H,CAI D D,CHEN Z Y,et al.Discovery of nortadalafil in health foods[J].Chinese Pharmaceutical Journal,2015,50(4):371-374.
[12] 林芳,李涛,王一欣,等.超高效液相色谱法同时测定降糖类保健食品中非法添加的13种化学药物[J].食品科学,2016,37(18):178-184.
LIN F,LI T,WANG Y X,et al.Simultaneous determination of 13 illegally added chemical drugs in hypoglycemic health foods by ultra performance liquid chromatography[J].Food Science,2016,37(18):178-184.
[13] 陈毓芳,林海丹,吴宏中,等.高效液相色谱法同时测定保健食品中11种功效成分[J].食品科学,2015,36(8):244-249.
CHEN Y F,LIN H D,WU H Z,et al.Simultaneous determination of 11 functional components in health foods by high performance liquid chromatography[J].Food Science,2015,36(8):244-249.
[14] ZHU X L,XIAO S,CHEN B,et al.Simultaneous determination of sildenafil,vardenafil and tadalafil as forbidden components in natural dietary supplements for male sexual potency by high-performance liquid chromatography-electrospray ionization mass spectrometry[J].Journal of Chromatography A,2005,1066(1-2):89-95.
[15] 许立,吴鸳鸯,寿林均,等.高效液相色谱-串联质谱法同时测定减肥类保健食品中27种违法添加化学药品[J].食品工业科技,2017,38(13):248-256;306.
XU L,WU Y Y,SHOU L J,et al.Simultaneous determination of 27 chemical drugs illegally added in slimming health food by high performance liquid chromatography-tandem mass spectrometry[J].Science and Technology of Food Industry,2017,38(13):248-256;306.
[16] 邓丽华.保健食品中他达那非类药物免疫检测方法研究[D].广州:华南农业大学,2 016.
DENG L H.Development of immunoassay for tadalafil and its analogues in health-care food[D].Guangzhou:South China Agricultural University,2016.
[17] 陈子键,曾道平,李美英,等.食品中褪黑素检测研究进展[J].食品工业,2015,36(11):233-238.
CHEN Z J,ZENG D P,LI M Y,et al.Advances in analytical method of melatonin in foods[J].The Food Industry,2015,36(11):233-238.
[18] 项昭保,伍晓玲,汪程远,等.色谱及其联用技术在保健食品非法添加药物检测中的应用研究进展[J].食品工业科技,2019,40(19):338-344.
XIANG Z B,WU X L,WANG C Y,et al.Research progress in the application of chromatography and its hyphenated techniques in the detection of illegally added drugs in health foods[J].Science and Technology of Food Industry,2019,40(19):338-344.
[19] 王静文,曹进,王钢力,等.保健食品中非法添加药物检测技术研究进展[J].药物分析杂志,2014,34(1):1-11.
WANG J W,CAO J,WANG G L,et al.Research progress on determination technologies for illegally added drugs in health food[J].Chinese Journal of Pharmaceutical Analysis,2014,34(1):1-11.
[20] 张丽霞,王学松.胶体金免疫层析技术在食品安全检测中的应用[J].广东化工,2021,48(1):180-181;163.
ZHANG L X,WANG X S.Application of colloidal gold immunochrom atography assay in food safety testing[J].Guangdong Chemical Industry,2021,48(1):180-181;163.
[21] 赵志高,骆骄阳,付延伟,等.免疫分析技术在农药残留分析中的研究进展及在中药中的应用展望[J].分析测试学报,2021,40(1):149-158.
ZHAO Z G,LUO J Y,FU Y W,et al.Research progress on immunoassay in pesticide residue analysis and its application prospect in chinese herbal medicine[J].Journal of Instrumental Analysis,2021,40(1):149-158.
[22] ZOU T T,YANG J Y,XU Z L,et al.Detection of tadalafil and its analogues in health wine and oral liquid by microwell lateral flow immunochromatography assay[J].Chinese Journal of Analytical Chemistry,2019,47(8):1 249-1 257.
[23] 刘源源,刘军,邹宇晓,等.抗疲劳功能食品检测评价研究进展及新思路[J].食品研究与开发,2016,37(2):209-213.
LIU Y Y,LIU J,ZOU Y X,et al.Research progress and new methods of detection and evaluation in anti-fatigue functional food[J].Food Research and Development,2016,37(2):209-213.
[24] 刘梯楼,孙双姣.金标记免疫共振散射光谱法测定牛初乳中免疫球蛋白G[J].湖南农业科学,2011(17):101-104.
LIU T L,SUN S J.Determination of IgG in bovine colostrum by using colloid gold labeling immune resonance scattering spectral assay[J].Hunan Agricultural Sciences,2011(17):101-104.
[25] 韩继美.胶体金免疫层析法对AFP、CEA和FN的快速检测[D].上海:上海师范大学,2009.
HAN J M.Development of rapid one-step colloidal gold immunochromatographic assay for detection of AFP,CEA and FN[D].Shanghai:Shanghai Normal University,2009.
[26] 刘会芹.免疫法检测D-二聚体的研究[D].青岛:青岛科技大学,2010.
LIU H Q.Study of D-dimer by immunity method[D].Qingdao:Qingdao University of Science and Technology,2010.
[27] 向知峰.基于胶体金标记的电化学免疫分析方法的研究[D].长沙:湖南师范大学,2005.
XIANG Z F.The study of the electrochemical immunoassay method based on the colloidal gold labeling[D].Changsha:Hunan Normal University,2005.
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