The endolysin and holin of phage are new bacteriostatic agents with great potential and prospect. Lysis activity and bacteria resistance for Lys 9, Lys 2, Hol 9 and Hol 2 were studied. The stability of Lys 9, Lys 2, Hol 9 and Hol 2 to temperature and pH were evaluated by plate count using endolysin and holin of Escherichia coli O157:H7 phage EC-P9 and Salmonella phage SM-P2. The combined antibacterial effect of endolysin, holin and their mixtures was also determined. The results showed that Lys 9, Lys 2, Hol 9 and Hol 2 had strong bactericidal ability and the highest lysing activity of bacteria were 61.348%, 81.120%, 74.251% and 83.356%, respectively. Lys 9, Lys 2, Hol 9 and Hol 2 had excellent stability in a wide range of temperature and pH. The bacteria did not develop resistance to Lys 9, Lys 2, Hol 9 and Hol 2 within 12 generations. In addition, endolysin and holin showed synergistic effect on the inhibition of bacteria. The experimental results provide a theoretical basis for the combined antibacterial effect of endolysin and holin in phage.
SHI Dongling
,
XIE Tianhui
,
SHI Hui
. Characteristics and combined bacteriostasis of endolysin and holin in phage EC-p9 and SM-p2[J]. Food and Fermentation Industries, 2021
, 47(24)
: 7
-12
.
DOI: 10.13995/j.cnki.11-1802/ts.027556
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