High speed countercurrent chromatography (HSCCC) is a rapid and efficient separation method based on liquid-liquid separation principle. In this article, HSCCC was investigated to separate maltosyl-β-cyclodextrin, maltose and β-cyclodextrin simultaneously. The partitioning of sugars was investigated in ethanol-salt aqueous two-phase systems addressing the effects of phase-forming salt type (NaH2PO4/K2HPO4/(NH4)2SO4) and concentration, organic reagents type and concentration. By measuring the partition coefficients of sugars in the upper and lower phases, it was found that the partition coefficients were the most different among maltosyl-β-cyclodextrin, β-cyclodextrin and maltose when NaH2PO4 and acetone were used, and the phase separation time was less than 30 s. V(300 g/L NaH2PO4)∶V(ethanol)∶V(acetone)=6∶2∶1.5 was optimized finally for the solvent system, and the above phase was the stationary phase and another was the mobile phase. The operating conditions were determined to be 800 r/min and with 2 mL/min of the flow rate. The effluent was collected every 15 minutes. In 30-35 min, the target compound was collected and analyzed by HPLC, the result was shown that the purity was up to 93.2% which increased by 61%, and the retention rate of the stationary phase was 60%. Therefore, HSCCC is a fast and efficient separation method, and it has broad application scenarios in the separation and purification of maltosyl-β-cyclodextrin.
WU Xue
,
QIU Chao
,
WANG Jinpeng
. Separation and purification of maltosyl-β-CD by high-speed counter-current chromatography[J]. Food and Fermentation Industries, 2021
, 47(24)
: 83
-88
.
DOI: 10.13995/j.cnki.11-1802/ts.027223
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