Food and Fermentation Industries >

2021 , Vol. 47 >Issue 24: 249 - 255

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027068

Simultaneous determination of malachite green and crystal violet residues in aquatic products by multi-plug filtration cleanup method combined with ultra-performance liquid chromatography-tandem mass spectrometry

  • GAO Xiaomin ,
  • WANG Jugang ,
  • MA Zhiling ,
  • YAN Chengming ,
  • CHEN Wuhai
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  • 1(College of Agro-forestry Engineering & Planning, Tongren University, Tongren 554300,China)
    2(Guizhou Provincial Key Laboratory for Biodiversity Conservation and Utilization in the Fanjing Mountain Region, Tongren University, Tongren 554300,China)
    3(South Subtropical Corp Research Institute,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang 524091, China)
    4(Zhanjiang Experiment Station,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang 524013, China)
    5(Agricultural Products Processing Research Institute,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang 524000, China)

Received date: 2021-03-02

  Revised date: 2021-04-22

  Online published: 2022-01-21

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Abstract

In this paper, a multi-plug filtration cleanup (m-PFC) method combined with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established for the simultaneous determination of malachite green (MG) and its metabolite of leucomalachite green (LMG), crystal violet (CV) and its metabolite residues of leucocrystal violet (LCV) in aquatic products. In this experiment, the pretreatment samples were directly extracted by acetonitrile and centrifuged. After frozen and removed fat, the supernatant was directly injected into the m-PFC column for purification in one step. The purification directly adsorbed impurities without activation, elution and concentration in less than 1 min. The results showed that the linear range of the four substances was in the range of 0.1-6.0 ng/mL, and the correlation coefficient (R2) was greater than 0.99. The detection limit was 0.05 μg/kg, and the quantitative limit was 0.2 μg/kg. The average recovery rate of the method was between 71.1%-84%, with the relative standard deviations (RSD) less than 14% at the spiking levels of 0.5, 1, 2 and 10 μg/kg. Compared with the traditional pretreatment method, the new method was simple, time-saving and high sensitivity. It provides an effective technical support for the determination of malachite green and crystal violet residues in aquatic products. It also provides a new idea for the development of a more rapid and accurate method for the detection of pesticide residues in aquatic products.

Key words： multi-plug filtration cleanup; UPLC-MS/MS; aquatic products; malachite green; leucomalachite green; crystal violet (CV); leucocrystal violet

Cite this article

GAO Xiaomin , WANG Jugang , MA Zhiling , YAN Chengming , CHEN Wuhai . Simultaneous determination of malachite green and crystal violet residues in aquatic products by multi-plug filtration cleanup method combined with ultra-performance liquid chromatography-tandem mass spectrometry[J]. Food and Fermentation Industries, 2021 , 47(24) : 249 -255 . DOI: 10.13995/j.cnki.11-1802/ts.027068

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