A yeast isolate with high yield of ethyl caproate: Screening, identification and fermentation optimization

  • QIN Liqin ,
  • YIN Huan ,
  • CHENG Liujie ,
  • GONG Yi ,
  • DING Ze ,
  • LI Xiuting ,
  • FAN Guangsen
Expand
  • 1(School of Food and Health,Beijing Technology and Business University,Beijing 100048,China)
    2(Beijing Advanced Innovation Center for Food Nutrition and Human Health,Beijing Technology and Business University,Beijing 100048,China)
    3(Beijing Engineering and Technology Research Center of Food Additives,Beijing Technology and Business University,Beijing 100048,China)

Received date: 2021-02-01

  Revised date: 2021-04-08

  Online published: 2022-01-27

Abstract

Ethyl caproate is a crucial flavor compound in strong-flavor Baijiu.By increasing the content of ethyl caproate the productivity of high-quality raw Baijiu will be increased. In this study, a yeast isolate, strain Y9, with high yield of ethyl caproate was isolated from different Jiuqu (starter for Baijiu) samples. Strain Y9 was systemically identified to be Hypopichia burtonii. It exhibited excellent tolerance to NaCl, glucose, ethanol and ethyl caproate and a wide range of pH, indicating that it very adapted the condition of strong-flavor Baijiu brewing. A high yield of ethyl caproate (15.0 mg/L) was obtained following the fermentation conditions: sugar content 14 °Brix, initial pH 5.0, induction temperature 22 ℃ static fermentation, 8% (v/v) ethanol, 0.04% (v/v) caproic acid for 60 h. These results provide a theoretical basis for the better future exploitation of aroma-producing H. burtonii Y9 in Baijiu fermentation.

Cite this article

QIN Liqin , YIN Huan , CHENG Liujie , GONG Yi , DING Ze , LI Xiuting , FAN Guangsen . A yeast isolate with high yield of ethyl caproate: Screening, identification and fermentation optimization[J]. Food and Fermentation Industries, 2022 , 48(1) : 55 -61 . DOI: 10.13995/j.cnki.11-1802/ts.026947

References

[1] ZHANG H M,MENG Y J,WANG Y L,et al.Prokaryotic communities in multidimensional bottom-pit-mud from old and young pits used for the production of Chinese strong-flavor Baijiu[J].Food Chemistry,2020,312:126084.
[2] LIU H L,SUN B G.Effect of fermentation processing on the flavor of Baijiu[J].Journal of Agricultural and Food Chemistry,2018,66(22):5 425-5 432.
[3] FAN G S,SUN B G,XU D,et al.Isolation and identification of high-yield ethyl acetate-producing yeast from Gujinggong Daqu and its fermentation characteristics[J].Journal of the American Society of Brewing Chemists,2018,76(2):117-124.
[4] 李俊辉, 刘英杰,隋丽娜,等.浓香型白酒增加己酸乙酯降低乳酸乙酯的研究进展[J].中国酿造,2019,38(1):1-4.
LI J H,LIU Y J,SUI L N,et al.Research progress on ethyl hexanoate-increasing and ethyl lactate-decreasing of strong-flavor Baijiu[J].China Brewing,2019,38(1):1-4.
[5] SU C,ZHANG K Z,CAO X Z,et al.Effects of Saccharomycopsis fibuligera and Saccharomyces cerevisiae inoculation on small fermentation starters in Sichuan-style Xiaoqu liquor[J].Food Research International,2020,137:109425.
[6] 王松, 陈雪玲,游玲.1株产香酵母在浓香型白酒复糟酒生产中的应用[J].中国酿造,2020,39(9):42-47.
WANG S,CHEN X L,YOU L.Application of aroma-producing yeast in the strong-flavor Baijiu production with reusing distiller’s grain[J].China Brewing,2020,39(9):42-47.
[7] WANG W H,FAN G S,LI X T,et al.Application of Wickerhamomyces anomalus in simulated solid-state fermentation for Baijiu production:Changes of microbial community structure and flavor metabolism[J].Frontiers in Microbiology,2020,11:598758.
[8] 宋瑞滨, 邵泽良,宋军.浓香型白酒生产中乙酸乙酯偏高的原因及控制措施[J].酿酒,2019,46(3):55-60.
SONG R B,SHAO Z L,SONG J.Analysis of the reasons and control measures for high ethyl acetate in Luzhou-flavor liquor production[J].Liquor Making,2019,46(3):55-60.
[9] 张强, 郭元,韩德明.酿酒酵母乙醇耐受性的研究进展[J].化工进展,2014,33(1):187-192.
ZHANG Q,GUO Y,HAN D M.Research progress in the ethanol tolerance of yeast[J].Chemical Industry and Engineering Progress,2014,33(1):187-192.
[10] ASWINI K,GOPAL N O,UTHANDI S.Optimized culture conditions for bacterial cellulose production by Acetobacter senegalensis MA1[J].BMC Biotechnology,2020,20(1):46.
[11] YOSHIMOTO H,FUJIWARA D,BOGAKI T,et al.Mechanisms of acetate ester production and control in yeasts[J].Journal of Bioscience and Bioengineering,2001,91(2):231.
[12] FUJII T,KOBAYASHI O,YOSHIMOTO H,et al.Effect of aeration and unsaturated fatty acids on expression of the Saccharomyces cerevisiae alcohol acetyltransferase gene[J].Applied and Environmental Microbiology,1997,63(3):910-915.
[13] 刘朋肖, 常煦,成柳洁,等.响应面法优化酿酒酵母Y3401产己酸乙酯发酵条件[J].中国食品学报,2021,已收录.
LIU P X,CHANG X,CHENG L J,et al.Optimization of cultural conditions for ethyl caproate production from Saccharomyces cerevisiae Y3401 by response surface methodology[J].Journal of Chinese Institute of Food Science and Technology,2021,已收录.
[14] KURIBAYASHI T,KANEOKE M,HIRATA D,et al.Analysis of free fatty acids in sake by an enzymatic method and its application for estimating ethyl caproate and selecting yeast with high productivity of the ester[J].Bioscience Biotechnology and Biochemistry,2012,76(2):391-394.
[15] 钟姝霞, 万世旅,边名鸿,等.一株高产乙酸乙酯酵母的鉴定及产酯条件的研究[J].中国酿造,2017,36(2):75-79.
ZHONG S X,WAN S L,BIAN M H,et al.Identification and ester-producing conditions of a high ethyl acetate-producing yeast[J].China Brewing,2017,36(2):75-79.
[16] HASHEM M,ALAMRI S A,ASSERI T A Y,et al.On the optimization of fermentation conditions for enhanced bioethanol yields from starchy biowaste via yeast co-cultures[J].Sustainability,2021,13(4):1890.
Outlines

/