Evaluation of antifreeze properties of bovine collagen in hamburger

  • XU Yuqian ,
  • GUO Mei ,
  • LIU Guishan ,
  • YUAN Jiangtao ,
  • GUO Jiajun
Expand
  • (School of Food&Wine, Ningxia University, Yinchuan 750021, China)

Received date: 2021-02-20

  Revised date: 2021-04-20

  Online published: 2022-02-28

Abstract

In order to evaluate the antifreeze properties of bovine collagen in hamburger, 2%, 4%, 6% and 8% bovine collagen were added into the hamburger to measure the following indexes including thawing loss rate, pH, color, salt soluble protein content, salt-extractable protein water holding capacity and Ca2+-adenosine triphosphatase (ATPase) activity after 7 d short term freezing storage, 30 d long term storage and 2, 4 and 6 freeze-thaw cycles storage. The results showed that with the prolongation of storage time, the thawing loss rate of the control group showed an upward trend. However, pH, color, salt soluble protein content, salt-extractable protein water holding capacity and Ca2+-ATPase activity showed a downward trend. The thawing loss rate decreased in treatment group with bovine collagen, and the maximum reached 15.74% when the addition was 6%. The pH value and color had changed not significantly. Salt soluble protein content, salt-extractable protein water holding capacity and Ca2+-ATPase activity all increased(P<0.05), and the treatment group increased by 25.79%, 34.95% and 25.71% at 6% respectively. The results showed that bovine collagen could effectively reduce the water loss and protein denaturation caused by freezing and thawing cycles, and improve the preservation rate of beef products in freezing.

Cite this article

XU Yuqian , GUO Mei , LIU Guishan , YUAN Jiangtao , GUO Jiajun . Evaluation of antifreeze properties of bovine collagen in hamburger[J]. Food and Fermentation Industries, 2022 , 48(2) : 182 -188 . DOI: 10.13995/j.cnki.11-1802/ts.027039

References

[1] 杨永安,李静静,刘建福,等.不同温度波动对冻藏三文鱼色差和质构的影响[J].食品与发酵工业, 2021,47(10):145-150.
YANG Y A, LI J J, LIU J F, et al.Temperature fluctuation on color difference and texture of frozen salmon[J].Food and Fermentation Industries, 2021,47(10):145-150.
[2] 程天赋, 蒋奕, 张翼飞,等.基于低场核磁共振研究不同解冻方式对冻猪肉食用品质的影响[J].食品科学, 2019, 40(7):20-26.
CHENG T F, JIANG Y, ZHANG Y F, et al.Effects of different thawing methods on quality of frozen pork as investigated using low field-nuclear magnetic resonance[J].Food Science, 2019, 40(7):20-26.
[3] 何艳, 刘彦言, 鲍文静, 等.不同解冻方法对冻结肉品质的影响[J].食品与发酵工业, 2018, 44(5):291-295.
HE Y, LIU Y Y, BAO W J, et al.Effects of different thawing methods on the quality of frozen meat[J].Food and Fermentation Industries, 2018, 44(5):291-295.
[4] 于淑池,谢家淑.复合抗冻剂的配方优化及对卵形鲳鲹冻藏品质的影响[J].食品科技,2020,45(7):267-274.
YU S C, XIE J S.Optimization of the formulation of composite cryoprotectant and its effects on the quality of frozen Trachinotus Ovatus[J].Food Science and Technology, 2020, 45(7):267-274.
[5] 李敏涵, 李洪军, 李少博, 等.抗冻保护剂在肉品及水产品贮藏保鲜中的应用研究进展[J].食品科学,2021,42(1):294-301.
LI M H, LI H J, LI S B, et al.Application of cryoprotectants in the frozen storage of meat and aquatic products:A review[J].Food Science,2021,42(1):294-301.
[6] DU L, BETTI M.Cryoprotective property of chicken collagen hydrolysate(CCH) on isolated actomyosin (IAM)[J].Meat Science, 2016(112):124.
[7] KOVACˇEVIC' D, MASTANJEVIC' K.Cryoprotective effect of trehalose and maltose on washed and frozen stored beef meat[J].Czech Journal of Food Sciences, 2011, 29(1):15-23.
[8] YANG S Y,KIM Y H,LEE M H.The effect of cryoprotectants on the quality changes of pork and beef during frozen storage[J].Korean Journal of Food Science and Technology(Korea R.), 1989, 21:364-369.
[9] 孟子晴, 赵改名, 祝超智, 等.牛肉饼中植物源添加物的配方优化及对牛肉肉糜品质的影响[J].现代食品科技, 2019, 35(12):166-175;76.
MENG Z Q, ZHAO G M, ZHU C Z, et al.Optimization of plant additive-containing formulations for beef patties and its effect on beef minced meat quality[J].Modern Food Science and Technology, 2019, 35(12):166-175;76.
[10] 宋蕾. 冻藏条件和糖类抗冻剂对调理肉丸品质的影响[D].南京:南京农业大学, 2016.
SONG L.Effects of frozen storage condition and carbohydrate cryoprotectants on the quality of prepared meatballs[D].Nanjing:Nanjing Agricultural University, 2016.
[11] 赵子瑞. 低钠酱牛肉制备配方优化与品质改良及贮藏特性研究[D].长春:吉林大学,2019.
ZHAO Z R.Study on the preparation formula optimization, quality improvement and storage characteristics of low sodium spiced beef[D].Changchun:Jilin University,2019
[12] 梁鹏, 钟机, 张浩, 等.复合抗冻剂对蓝圆鯵冷冻鱼糜抗冻效果的影响[J].福建农林大学学报(自然科学版), 2015, 44(6):618-623.
LIANG P, ZHONG J, ZHANG H, et al.Effect of composite antifreeze on Decapterus maruadsi surimi during frozen storage[J].Journal of Fujian Agriculture and Forestry University (Natural Science Edition), 2015, 44(6):618-623.
[13] NAING A H, KIM C K.A brief review of applications of antifreeze proteins in cryopreservation and metabolic genetic engineering[J].3 Biotech, 2019, 9(9):329.
[14] 钱书意, 李侠, 孙圳, 等.不同冻结温度下牛肉的肌原纤维蛋白变性与肌肉持水性[J].食品科学,2018,39(15):24-30.
QIAN S Y, LI X, SUN Z, et al.Myofibrillar protein denaturation and water-holding capacity of beef frozen at different temperatures[J].Food Science, 2018, 39(15):24-30.
[15] WU X F, ZHANG M, ADHIKARI B, et al.Recent developments in novel freezing and thawing technologies applied to foods[J].Critical Reviews in Food Science and Nutrition, 2017, 57(17):3 620-3 631.
[16] 蒙健宗, 秦小明, 赵文报, 等.海藻糖对冷冻罗非鱼片蛋白质变性作用的影响[J].食品工业科技, 2007, 28(2):214-216.
MENG J Z, QIN X M, ZHAO W B, et al.Effect of trehalose on protein denaturation of frozen tilapia fillets[J].Science and Technology of Food Industry, 2007, 28(2):214-216.
[17] ZHANG M, LI F, DIAO X, et al.Moisture migration, microstructure damage and protein structure changes in porcine longissimus muscle as influenced by multiple freeze-thaw cycles[J].Meat Science, 2017, 133:10-18.
[18] 李磊, 崔丽伟, 张雪, 等.乳清粉在鲜切牛排涂膜中的应用研究[J].食品科技, 2019, 44(1):174-179.
LI L, CUI L W, ZHANG X, et al.The application of whey in the coating of fresh-cut steak[J].Food Science and Technology, 2019, 44(1):174-179.
[19] 董福凯, 查恩辉, 张振, 等.不同包装方式调理牛排在冰温贮藏过程中品质变化特点[J].食品工业科技, 2019, 40(8):247-253.
DONG F K, CHA E H, ZHANG Z, et al.Changes characteristics in the quality of conditioning steak in different packaging methods during ice-temperature storage[J].Science and Technology of Food Industry, 2019, 40(8):247-253.
[20] 明丹丹, 张一敏, 董鹏程, 等.牛肉肉色的影响因素及其控制技术研究进展[J].食品科学, 2020, 41(1):284-291.
MING D D, ZHANG Y M, DONG P C, et al.Recent progress in studies of factors influencing beef color and technologies for controlling it[J].Food Science, 2020, 41(1):284-291.
[21] 李金平, 李春保, 徐幸莲, 等.反复冻融对牛外脊肉品质的影响[J].江苏农业学报, 2010, 26(2):406-410.
LI J P, LI C B, XU X L, et al.Effect of freeze-thaw cycleon meat quality of beef striploin[J].Jiangsu Journal of Agricultural Sciences, 2010,26(2):406-410.
[22] QI J, LI C, CHEN Y, et al.Changes in meat quality of ovine longissimus dorsi muscle in response to repeated freeze and thaw[J].Meat Science, 2012, 92(4):619-626.
[23] SEO J H, NAING A H, JEON S M, et al.Anti-freezing-protein type III strongly influences the expression of relevant genes in cryopreserved potato shoot tips[J].Plant Molecular Biology, 2018, 97(4):347-355.
[24] LEE H H, LEE H J, KIM H J, et al.Effects of antifreeze proteins on the vitrification of mouse oocytes:Comparison of three different antifreeze proteins[J].Human Reproduction, 2015, 30(9):2 110-2 119.
[25] LEYGONIE C, BRITZ T J, HOFFMAN L C.Impact of freezing and thawing on the quality of meat[J].Meat Science, 2012, 91(2):93-98.
[26] 欧阳杰, 倪锦, 吴锦婷, 等.解冻方式对大黄鱼解冻效率和品质的影响[J].肉类研究, 2016,30(8):30-34.
OUYANG J, NI J, WU J T, et al.Influence of thawing methods on thawing efficiency and quality of Pseudosciaena crocea[J].Meat Research, 2016, 30(8):30-34.
[27] ENGLISH M M, KEOUGH J M, MCSWEENEY M B, et al.Impact of a novel cryoprotectant blend on the sensory quality of frozen lobster(Homarus americanus)[J].Journal of Food Science, 2019, 84(6):1 547-1 553.
[28] LIU M, LIANG Y, WANG Y N, et al.Effects of recombinant carrot antifreeze protein from Pichia pastoris GS115 on the physicochemical properties of hydrated gluten during freeze-thawed cycles[J].Journal of Cereal Science, 2018, 83:245-251.
[29] 陈思, 李婷婷, 李欢, 等.白鲢鱼片在冷藏和微冻条件下的鲜度和品质变化[J].食品科学, 2015, 36(24):297-301.
CHEN S, LI T T, LI H, et al.Changes in freshness and quality of silver carp fillets during chilled and partial freezing storage[J].Food Science, 2015, 36(24):297-301.
[30] 王宁. 复合抗冻剂对大黄花鱼抗冷冻变性及持水性的研究[D].锦州:锦州医科大学,2018.
WANG N. The research of compound antifreeze on the antifreeze and hydrophobicity of frozen larimichthys crocea[D].Jinzhou:Jinzhou Medical University,2018.
Outlines

/