Food and Fermentation Industries >

2022 , Vol. 48 >Issue 2: 189 - 197

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027695

Comparative study on the physicochemical properties and biological activities of polysaccharides from different components in the berry of Berberi dasystachya

  • ZHOU Wen ,
  • HAN Lijuan ,
  • MA Nana ,
  • QI Ying ,
  • YUE Qingming ,
  • SUO Nanzhuoma ,
  • GUO Yan ,
  • YUAN Zhenzhen
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  • 1(College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China)
    2(State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China)

Received date: 2021-04-12

  Revised date: 2021-05-10

  Online published: 2022-02-28

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Abstract

The hypoglycemic effects of different components of Berberi dasystachya polysaccharides (BDPs) were studied comprehensively by combining the effects of antioxidation, hypoglycemia and regulation of intestinal flora in vitro. Different components of BDPs(BDPs-70, BDPs-80 and BDPs-90)were prepared by ethanol alcohol precipitation and their primary structures were characterized by high performance gel permeation chromatography, Fourier transform infrared spectroscopy and scanning electron microscopy. Comparing DPPH and ABTS free radical scavenging rate, reducing power, α-amylase and α-glucosidase inhibition rate and their effects on Lactobacillus acidophilus and Escherichia coli were compared. The results showed that the uronic acid content of BDPs-80 was the highest, and the average molecular weights of BDPs-70, BDPs-80 and BDPs-90 were 9 851, 9 901 and 9 855 Da, respectively. It showed characteristic absorption peaks of polysaccharides, which were β-pyran polysaccharides with flaky surface structure. The scavenging rates of the three polysaccharides on DPPH and ABTS free radicals were in the following order: BDPs-90>BDPs-80>BDPs-70, and the reducing power of BDPs-80 was the best. The inhibition rate of α-amylase was BDPs-90>BDPs-80>BDPs-70. The inhibition rate of α-glucosidase was BDPs-80>BDPs-90>BDPs-70. The three polysaccharides as carbon sources can promote the proliferation of Lactobacillus acidophilus (BDPs-90 has the best promotion effect), significantly increase the content of short chain fatty acids, and inhibit the growth of Escherichia coli (BDPs-80 has the best inhibitory effect). The theoretical basis for the development of Berberi dasystachya polysaccharides into food and medicine for the prevention and treatment of diabetes were provided in this research.

Key words： Berberi dasystachya polysaccharides; ethanol fractional precipitation; antioxidant activity; hypoglycemic effect; gut microbes

Cite this article

ZHOU Wen , HAN Lijuan , MA Nana , QI Ying , YUE Qingming , SUO Nanzhuoma , GUO Yan , YUAN Zhenzhen . Comparative study on the physicochemical properties and biological activities of polysaccharides from different components in the berry of Berberi dasystachya[J]. Food and Fermentation Industries, 2022 , 48(2) : 189 -197 . DOI: 10.13995/j.cnki.11-1802/ts.027695

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