Food and Fermentation Industries >

2022 , Vol. 48 >Issue 3: 92 - 98

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027530

Antioxidant and hypoglycemic activities of different polar phases of bitter bamboo shoot husk extract

  • MENG Ailian ,
  • CHEN Yuanyuan ,
  • MA Yuan ,
  • LIU Xiaocui ,
  • WEN Xueju ,
  • LIU Ping ,
  • YUAN Anqi ,
  • YE Kunyue
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  • (School of Food and Bioengineering,Xihua University,Chengdu 611743,China)

Received date: 2021-03-27

  Revised date: 2021-04-14

  Online published: 2022-03-04

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Abstract

To study the antioxidant effects of different extraction phases of bitter bamboo shoots shells and their inhibitory effects on α-glucosidase and α-amylase activities, the bitter bamboo shoots shells were extracted with 70% ethanol and water, and the flavonoids, total phenols, and total sugars in the extract were determined. The antioxidant activity was evaluated by radical ion ABTS+, DPPH·, ·OH scavenging ability and FRAP antioxidant capacity. The inhibitory activities of ethyl acetate, n-butyl alcohol and petroleum ether against α-glucosidase and α-amylase were determined. The results showed that the total sugar content of water extraction was higher (97.3±0.28)% than that of alcohol extraction. The contents of total phenols and flavonoids in the alcohol extraction phase were (28.53±1.67)% and (27.22±2.02)%, respectively. In terms of the inhibitory activity of α-glucosidase, the alcohol extraction phase was higher than the water extraction phase. The antioxidant activity under alcohol extraction was as follows: ethyl acetate phase> n-butyl alcohol phase>petroleum ether phase. The ethyl acetate relative ·OH scavenging ability reached (67.32±1.57)%, the ethyl acetate had the strongest inhibitory activity to α-glucosidase and α-amylase (IC50 value was (0.158±0.003) and (0.104±0.006) mg/mL, respectively). The kinetic results of the inhibition of bitter bamboo shoots shells: protease phase was competitive inhibition type relative to α-glucosidase inhibition type, and non-competitive inhibition type to α-amylase inhibition type. Bitter bamboo shoots shells can become a natural resource for the development of hypoglycemic products.

Key words： bitter bamboo shoot shell; active ingredient; antioxidant capacity; hypoglycemic; types of enzyme inhibition

Cite this article

MENG Ailian , CHEN Yuanyuan , MA Yuan , LIU Xiaocui , WEN Xueju , LIU Ping , YUAN Anqi , YE Kunyue . Antioxidant and hypoglycemic activities of different polar phases of bitter bamboo shoot husk extract[J]. Food and Fermentation Industries, 2022 , 48(3) : 92 -98 . DOI: 10.13995/j.cnki.11-1802/ts.027530

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