Food and Fermentation Industries >

2022 , Vol. 48 >Issue 3: 130 - 134

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028485

Acetylation modification of Lentinus edodes stipe polysaccharide and its antioxidant activity

  • LI Shunfeng ,
  • XU Fangfang ,
  • CUI Guomei ,
  • TIAN Guangrui ,
  • GAO Shuaiping ,
  • LIU Lina ,
  • WANG Anjian ,
  • WEI Shuxin
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  • (Research Center of Agro-products Processing Science and Technology,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)

Received date: 2021-07-04

  Revised date: 2021-08-19

  Online published: 2022-03-04

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Abstract

Using Lentinus edodes stipe as raw material, polysaccharide of L. edodes stipe was acetylated with acetic anhydride to yield acetylated polysaccharide. The effects of different acetic anhydride dosage on acetylated degree of substitution and structural characteristics of polysaccharide in NaOH system and formamide system were investigated, and the antioxidant activity of polysaccharide and acetylated polysaccharide were also evaluated. The results showed that the degree of substitution for acetylation modification of the polysaccharide was positively correlated with the amount of acetic anhydride in both NaOH system and formamide system. When the amount of acetic anhydride was 5 mL, the degrees of substitution were 0.31 and 0.14, respectively. The infrared spectrum results showed that the acetylated polysaccharide had not only the characteristic peak of polysaccharide, but also the characteristic absorption peak of acetylation group, which indicated that the acetylation modification of the polysaccharide was successful. After acetylation, the polysaccharide still had the triple helix structure in NaOH system, however, it was destroyed in formamide system. The antioxidant results showed that the polysaccharide had a certain antioxidant capacity no matter it was acetylated modification, which showed a certain dose-effect relationship as well. The antioxidant activity of acetylated polysaccharide in NaOH system was stronger than that in L. edodes stipe polysaccharide and formamide system. The results can be concluded that NaOH system was appropriate to acetylated modification for polysaccharide of L. edodes stipe.

Key words： Lentinus edodes stipe polysaccharide; acetylation modification; degree of substitution; infrared spectrum; antioxidant activity

Cite this article

LI Shunfeng , XU Fangfang , CUI Guomei , TIAN Guangrui , GAO Shuaiping , LIU Lina , WANG Anjian , WEI Shuxin . Acetylation modification of Lentinus edodes stipe polysaccharide and its antioxidant activity[J]. Food and Fermentation Industries, 2022 , 48(3) : 130 -134 . DOI: 10.13995/j.cnki.11-1802/ts.028485

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