Food and Fermentation Industries >

2022 , Vol. 48 >Issue 4: 102 - 108

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027640

Breeding of aroma-producing Kluyveromyces marxianus by atmospheric and room-temperature plasma mutagenesis

  • WANG Weixiong ,
  • HU Shaokun ,
  • GULIMIRE Zununa ,
  • LI Jinxue ,
  • WANG Yanling ,
  • LYU Ze ,
  • DU Zhancheng ,
  • ZHANG Haijun ,
  • WU Yun
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  • 1(College of Food Science and Pharmacy, Xinjiang Agriculturai University, Urmuqi 830052, China)
    2(Xinjiang Aroma Manor Wine Co.Ltd., Heshuo 841200, China)
    3(Turpan Loulan Chateua Co.Ltd., Turpan 838201, China)

Received date: 2021-04-07

  Revised date: 2021-06-10

  Online published: 2022-03-16

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Abstract

The growth and metabolism of non-Saccharomyces cerevisiae can contribute to the flavor of wine by producing flavor substances, but the weak fermentation ability and low tolerance limit its application in alcohol fermentation. Atmospheric and room-temperature plasma (ARTP) mutagenesis was used to mutagenize Kluyveromyces marxianus YK selected from the surface of grape skins. Two mutagenic strains with high tolerance and strong aroma-producing ability were obtained through the preliminary screening for the tolerance of mutagenic strains and the secondary screening for aroma production. The genetic stability of the mutagenized strains was tested for five generations, which showed that the two mutant strains YK-1 and YK-29 had strong genetic stability. Two mutant strains increased the alcohol production capacity by 149% and 169%, respectively, compared with that of the original strain YK. Through quantitative descriptive analysis, the wine fermented by the mutagenized strain had more obvious berry and fruit aromas than the original strain. The conclusions provide theoretical support for the later mixed fermentation of non-Saccharomyces cerevisiae and Saccharomyces cerevisiae, which is expected to break the monotonic situation of wine quality and have a broad application prospect.

Key words： non-Saccharomyces cerevisiae; atmospheric and room-temperature plasma (ARTP); breeding; tolerance; aroma-producing

Cite this article

WANG Weixiong , HU Shaokun , GULIMIRE Zununa , LI Jinxue , WANG Yanling , LYU Ze , DU Zhancheng , ZHANG Haijun , WU Yun . Breeding of aroma-producing Kluyveromyces marxianus by atmospheric and room-temperature plasma mutagenesis[J]. Food and Fermentation Industries, 2022 , 48(4) : 102 -108 . DOI: 10.13995/j.cnki.11-1802/ts.027640

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