Food and Fermentation Industries >

2022 , Vol. 48 >Issue 4: 185 - 192

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.029199

The analysis of the antioxidant activities and chemical constituents of Tuber pesudohimalayense

  • LI Xinai ,
  • GUO Wenxiu ,
  • ZHANG Ye ,
  • ZHANG Xu ,
  • YIN Sheng ,
  • WANG Xinyu ,
  • GUO Dale ,
  • DENG Yun
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  • 1(School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China)
    2(Huidong Takachuan Tianyuan Agricultural Technology Co.Ltd., Liangshan Yi Autonomous Prefecture 615000, China)

Received date: 2021-09-02

  Revised date: 2021-09-29

  Online published: 2022-03-16

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Abstract

Through ultra-performance liquid chromatography- tandem q-exactive quadrupole-electrostatic field track trap high resolution mass spectrometry(UPLC-Q-Orbitrap HRMS HRMS) analysis and in vitro antioxidant activity (DPPH radicals, ABTS cationic radicals, hydroxyl scavenging rate, reducing power and total phenolic content) determination, the main nutrients of the 80% methanol extract of the Tuber pesudohimalayense and the antioxidant capacity of different solvents of the fruiting body (80% methanol, water) extract were preliminarily studied. A total of 51 components were identified from T.pesudohimalayense, including 13 free amino acids, 11 organic acids, 8 nucleosides, 9 amides, and 10 other types, 8 components (phenylalanine, leucine, L-glutamic acid, L-tyrosine, adenosine, benzoic acid, oleamide, pyrazine) were unambiguously identified by comparing with reference standards. The antioxidant results showed that the water extract (H2O) had higher scavenging and reducing power activities against DPPH free radicals, ABTS free radicals, and hydroxyl free radicals. The EC50 values were 14.97, 0.19, 3.21 mg/mL, respectively. The total phenol content of the water extract (H2O) was the highest (3.99 mg/g), and the total phenol content of the methanol extract (ME) was 1.87 mg/g. The study showed that the total phenol content and antioxidant activity in different extracts of truffles were different, and the total phenol content and antioxidant activity showed a positive correlation. This research could lay the foundation for the quality evaluation of truffles, the basic research of chemical composition and pharmacology, which providing a scientific basis for the development of natural antioxidants, and guiding significance for the development and utilization of truffle resources.

Key words： Tuber pesudohimalayense; ultra-performance liquid chromatography- tandem q-exactive quadrupole-electrostatic field track trap high resolution mass spectrometry; amino acid; total phenols; antioxidant activity

Cite this article

LI Xinai , GUO Wenxiu , ZHANG Ye , ZHANG Xu , YIN Sheng , WANG Xinyu , GUO Dale , DENG Yun . The analysis of the antioxidant activities and chemical constituents of Tuber pesudohimalayense[J]. Food and Fermentation Industries, 2022 , 48(4) : 185 -192 . DOI: 10.13995/j.cnki.11-1802/ts.029199

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