Food and Fermentation Industries >

2022 , Vol. 48 >Issue 4: 235 - 242

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027851

Research on the free radical scavenging ability and physicochemical properties of the polysaccharides in Gelidium crinale

  • PEI Yu ,
  • YANG Shengtao ,
  • FENG Rui ,
  • ZHOU Chunxia ,
  • HONG Pengzhi
Expand
  • 1(College of Food Science and Technology, Guangdong Ocean University, Guangdong Key Laboratory of Aquatic Product Processing and Safety, Guangdong Marine Food Engineering Technology Research Center, Zhanjiang 524088, China)
    2(Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524025, China)

Received date: 2021-04-29

  Revised date: 2021-05-11

  Online published: 2022-03-16

Fold

Abstract

Gelidium crinaleis a marine economic red algae belonging to the Gelidium. By studying the scavenging ability and physical and chemical properties of its polysaccharides extracts on free radicals, it provides an experimental basis for the high-value processing and utilization of Gelidium crinale. In this study, Gelidium crinale was used as raw material and polysaccharides were extracted from Gelidium crinale with 0.1 mol/L HCl. Its physical and chemical properties and antioxidant activity were studied. The results showed that the proportion of total sugars and reducing sugar in polysaccharides were 70.51% and 8.42%, respectively. Moreover, the proportion of sulfate group and protein were 16.50% and 5.27%. Analysis of monosaccharide composition showed that the top three were galactose, fucose and xylose which with a relative molecular weight of 25.8 kDa. The results of in vitro antioxidant results showed that polysaccharides from Gelidium crinale had good antioxidant activity. The IC50 of its ABTS free radical scavenging ability, Fe2+ chelating ability, DPPH free radical scavenging ability and hydroxyl free radical scavenging ability were 2.215, 2.688, 7.408, 13.56 mg/mL, respectively. The results of cell experiments showed that the level of superoxide dismutase (SOD) in cells was significantly increased in a dose-dependent manner after treatment with polysaccharide from Gelidium crinale, but it inhibited the production of reactive oxygen species (ROS) (P<0.001).

Key words： Gelidium crinale; polysaccharide; physicochemical properties; anti-oxidation

Cite this article

PEI Yu , YANG Shengtao , FENG Rui , ZHOU Chunxia , HONG Pengzhi . Research on the free radical scavenging ability and physicochemical properties of the polysaccharides in Gelidium crinale[J]. Food and Fermentation Industries, 2022 , 48(4) : 235 -242 . DOI: 10.13995/j.cnki.11-1802/ts.027851

References

[1] YANG T H, CHIU C Y, LU T J, et al.The anti-obesity effect of polysaccharide-rich red algae (Gelidium amansii) hot-water extracts in high-fat diet-induced obese hamsters[J].Marine Drugs, 2019, 17(9):532.
[2] 裴若楠, 翟红蕾, 戚勃, 等.红藻多糖的生物活性及应用研究进展[J].食品工业科技, 2019, 40(23):331-336.
PEI R N, ZHAI H L, QI B, et al.Research progress of bioactivity and application of polysaccharides from red algae[J].Food Industry Technology, 2019, 40(23):331-336.
[3] AZIZ E, BATOOL R, KHAN M U, et al.An overview on red algae bioactive compounds and their pharmaceutical applications[J].Journal of complementary & integrative medicine, 2020,17(4):20190203.
[4] DE CASTRO J P L, COSTA L E C, PINHEIRO M P.Polysaccharides of red alga Gracilaria intermedia:Structure, antioxidant activity and rheological behavior[J].Polímeros, 2018, 28(2):178-186.
[5] MITTLER R, VANDERAUWERA S, SUZUKI N, et al.ROS signaling:The new wave? [J].Trends in Plant Science, 2011, 16(6):300-309.
[6] GUPTA R K, PATEL A K, SHAH N, et al.Oxidative stress and antioxidants in disease and cancer:A review[J].Asian Pacific Journal of Cancer Prevention, 2014, 15(11):4 405-4 409.
[7] DOMINGUETI C P, DUSSE L M S A, CARVALHO M D G, et al.Diabetes mellitus:The linkage between oxidative stress, inflammation, hypercoagulability and vascular complications[J].Journal of diabetes and its complications, 2016, 30(4):738-745.
[8] FARIA A, PERSAUD S J.Cardiac oxidative stress in diabetes:Mechanisms and therapeutic potential[J].Pharmacology and Therapeutics, 2017, 172:50-62.
[9] SAHA S K, LEE S B, WON J, et al.Correlation between oxidative stress, nutrition, and cancer initiation[J].International journal of molecular sciences, 2017, 18(7):1 544.
[10] ZHANG D H, WU H X, XIA Z M, et al.Partial characterization, antioxidant and antitumor activities of three sulfated polysaccharides purified from Bullacta exarata[J].Journal of Functional Foods, 2012, 4(4):784-792.
[11] JIA X J, ZHANG C, HU J, et al.Ultrasound-assisted extraction, antioxidant and anticancer activities of the polysaccharides from Rhynchosia minima root[J].Molecules, 2015, 20(11):20 901-20 911.
[12] SUN Y J, HOU S T, SONG S, et al.Impact of acidic, water and alkaline extraction on structural features, antioxidant activities of Laminaria japonica polysaccharides[J].International Journal of Biological Macromolecules, 2018, 112:985-995.
[13] 林沛纯, 谌素华, 王维民, 等.亨氏马尾藻岩藻聚糖结构鉴定及其对氧化型低密度脂蛋白诱导HMVEC损伤的保护作用[J].广东海洋大学学报, 2020, 40(5):72-80.
LIN P C, ZHAN S H, WANG W M, et al.Structural identification of Sargassum henslowianum C.Agardh fucoidannd its protective effect on HMVEC damage induced by qxidized low density lipoprotein[J].Journal of Guangdong Ocean University, 2020, 40(5):72-80.
[14] 高文军, 李卫红, 王喜明, 等.3,5-二硝基水杨酸法测定蔓菁中还原糖和总糖含量[J].中国药业, 2020, 29(9):113-116.
GAO W J, LI W H, WANG X M, et al.Determination of reducing sugar and total sugar in turnip by 3,5-dinitrosalicylic acid colorimetry[J].China Pharmaceutical, 2020, 29(9):113-116.
[15] 裴若楠, 翟红蕾, 戚勃, 等.石花菜多糖的分离纯化及单糖组成分析[J].食品与发酵工业, 2020, 46(7):57-62.
PEI R N, ZHAI H L, QI B, et al.Isolation,purification and monosaccharide composition analysis of polysaccharide from Gelidium amansii[J].Food and Fermentation Industry, 2020, 46(7):57-62.
[16] JIA X J, ZHANG C, QIU J F, et al.Purification, structural characterization and anticancer activity of the novel polysaccharides from Rhynchosia minima root[J].Carbohydrate Polymers, 2015, 132:67-71.
[17] WANG Z C, LIU X Y, BAO Y R, et al.Characterization and anti-inflammation of a polysaccharide produced by Chaetomium globosum CGMCC 6882 on LPS-induced RAW 264.7 cells[J].Carbohydrate Polymers, 2021, 251:117129.
[18] AGRAWAL H, JOSGI R, GUPTA M.Isolation and characterisation of enzymatic hydrolysed peptides with antioxidant activities from green tender sorghum[J].LWT, 2017, 84:608-616.
[19] 萧振邦, 黄晓洋, 陈佳丽, 等.鲍鱼肠多肽对LPS诱导HUVEC炎症的抑制作用[J].广东海洋大学学报, 2020,40(6):108-115.
XIAO Z B, HUANG X Y, CHEN J L, et al.Effect of abalone polypeptide on LPS induced HUVEC cell inflammation[J].Journal of Guangdong Ocean University, 2020,40(6):108-115.
[20] YU J, LIU S, WU B, et al.Comparison of cytotoxicity and inhibition of membrane ABC transporters induced by MWCNTs with different length and functional groups[J].Environmental Science & Technology, 2016, 50(7):3 985-3 994.
[21] 杨胜涛, 陈佳丽, 刘怡, 等.异红藻糖苷对HT1080肿瘤细胞侵袭转移作用的影响[J].广东海洋大学学报, 2020, 40(6):116-122.
YANG S T, CHEN J L, LIU Y, et al.Effects of D-isofloridoside on invasion and metastasis of HT1080 tumor cells[J].Journal of Guangdong Ocean University, 2020, 40(6):116-122.
[22] 崔明晓. 大石花菜多糖的分离、结构表征及其抗炎活性研究[D].上海:上海海洋大学, 2019.
CUI X M.Isolation, characterization and anti-inflammatory activity of Gelidium pacificum Okamura polysaccharides[D].Shanghai:Shanghai Ocean University, 2019.
[23] 裴若楠. 石花菜多糖的提取、分离纯化及其生物活性研究[D].上海:上海海洋大学, 2020.
PEI R N.Isolation, purification and bioactivitier of polysaccharide from Gelidium amansii[D].Shanghai:Shanghai Ocean University, 2020.
[24] CUI M X, WU J W, WANG S Y, et al.Characterization and anti-inflam-matory effects of sulfated polysaccharide from the red seaweed Gelidium pacificum Okamura[J].International Journal of Biological Mac-romolecules, 2019, 129:377-385.
[25] 赵慧. 石花菜多糖的提取、分离纯化、理化性质及生物活性研究[D].南京:南京农业大学, 2018.
ZHAO H.Extraction, isolation, purification, physicochemical properties and biological activity of polysaccharide from Gelidium amansii[D].Nanjing:Nanjing Agricultural College, 2018.
[26] HOU C Y, CHEN L L, YANG L Z, et al.An insight into anti-inflammatory effects of natural polysaccharides[J].International Journal of Biological Macromolecules, 2020, 153:248-255.
[27] 崔明晓, 王晓晨, 王玉,等.超声辅助提取大石花菜多糖及其抗氧化研究[J].上海海洋大学学报, 2018, 27(5):797-804.
CUI X M, WANG X C, WANG Y, et al.Ultrasonic-assisted extraction and anti-oxidation of polysaccharides from Gelidium pacifium Okam.[J].Journal of Shanghai Ocean University 2018, 27(5):797-804.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.