Food and Fermentation Industries >

2022 , Vol. 48 >Issue 5: 28 - 23

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027926

Breeding of industrial fermentation yeast with low yield of higher alcohol

  • FENG Pengpeng ,
  • ZHOU Yuhan ,
  • GAO Xing ,
  • GAO Ying ,
  • GUO Liyun ,
  • GE Junling ,
  • LIN Liangcai ,
  • ZHANG Cuiying
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  • 1(State Key Laboratory Nutrition and Safety,Tianjin 300457,China)
    2(Key Laboratory of Fermentation Microbiology of Ministry of Education,Tianjin 300457,China)
    3(Tianjin Key Laboratory of Industrial Microbiology,Tianjin 300457,China)
    4(College of Biotechnology, Tianjin University of Science and Technology,Tianjin 300457,China

Online published: 2022-04-06

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Abstract

In order to obtain the industrial Saccharomyces cerevisiae strains with lower yield of higher alcohols, artificial evolution by atmospheric room temperature plasma (ARTP) was carried out. The high-throughput screening was also developed, which combined microplate culture system and spectrophotometry analysis. After two rounds of screening, eight mutants with lower yield of high alcohol were obtained. Among them, ARTP-162 showed the lowest higher alcohol concentration, which was decreased by around 21%. Furthermore, this mutant remained genetically stable after eight generations.

Key words： Saccharomyces cerevisiae; higher alcohols; high-throughput screening; genetic properties; ARTP

Cite this article

FENG Pengpeng , ZHOU Yuhan , GAO Xing , GAO Ying , GUO Liyun , GE Junling , LIN Liangcai , ZHANG Cuiying . Breeding of industrial fermentation yeast with low yield of higher alcohol[J]. Food and Fermentation Industries, 2022 , 48(5) : 28 -23 . DOI: 10.13995/j.cnki.11-1802/ts.027926

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