Response surface method was used to optimize the hydroiysis conditions of Pinctadafucata. The effects of the dose of protease, liquid-material ratio, enzymolysis time and enzymolysis temperature were investigated by degree of hydrolysis. The results showed that the optimized conditions were as follows : 5680 U/g of the amount of enzyme, liquid-material ratio 2.8:1(V：m), 6.5 h ofenzymolysis time, 55 ℃ of the enzymolysis temperature. Under this optimized conditions, the degree of hydrolysis was 44.27±1.23% which was almost same with the theoretical value. The hydrolysis process was simple, reliable, high hydrolysis rate and could beapply to practical production .