Food and Fermentation Industries >

2022 , Vol. 48 >Issue 5: 142 - 149

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027908

The differential analysis of antioxidant capacity and main functional components content of leaf extraction from different Ginkgo biloba male plants

  • XU Jiye ,
  • YU Wanwen ,
  • CAO Fuliang ,
  • WANG Guibin
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  • 1(Co-Innovation Center for the Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing 210037,China)
    2(The Jiangsu Province Platform for Construction and Utilization of Agricultural Germplasm,Nanjing 210014,China)

Online published: 2022-04-06

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Abstract

In this study, 23 males gingkoes leaves were used as materials to study the antioxidant capacity and the contents of functional components(total flavonoids, terpene lactones, polysaccharides, proanthocyanidins) between Ginkgo biloba individuals’ methanol extract. Meanwhile, their differences in antioxidant capacity and the contents of functional components were analyzed. Finally, an excellent single plant with the better antioxidant function was screened out by cluster analysis. The results showed that the antioxidant capacity and functional component content of leaf extract were significantly different between individuals (P＜0.01). Rich genetic diversity was found in G.biloba male plants, the variation coefficient of antioxidant capacity and functional components contents ranged from 13.5% to 37.04%, from 9.3% to 15.63%, respectively.Moreover, DPPH scavenging rate, ·O-2 scavenging rate, total reducing the power of leaf extract were significantly positively correlated with total flavonoids content(P＜0.01), the correlation coefficients were 0.52、0.70、0.62 respectively. The results indicated that total flavonoids were the main material basis of antioxidant capacity of G.biloba leaf extract. Furthermore, 23 G.biloba male plants were divided into 4 types by cluster analysis,and only 6# male plant showed high functional components contents and high antioxidant activity. These results provide a scientific basis for the screening of high antioxidant ginkgo germplasm and breeding high antioxidant ginkgo new varieties.

Key words： Ginkgo biloba male plant; leaf extraction; antioxidant capacity; functional components

Cite this article

XU Jiye , YU Wanwen , CAO Fuliang , WANG Guibin . The differential analysis of antioxidant capacity and main functional components content of leaf extraction from different Ginkgo biloba male plants[J]. Food and Fermentation Industries, 2022 , 48(5) : 142 -149 . DOI: 10.13995/j.cnki.11-1802/ts.027908

References

[1] 徐艳芬,张丽娟,宋新波.银杏叶提取物的研究进展[J].药物评价研究,2010,33(6):452-456.
XU Y F,ZHANG L J,SONG X B.Advances in research on extract of Ginkgo biloba leaves[J].Drug Evaluation Research,2010,33(6):452-456.
[2] 李艳萍,张立虎,吴红雁,等.银杏叶功效群组分及其功效的研究进展[J].食品研究与开发,2020,41(15):182-187.
LI Y P,ZHANG L H,WU H Y,et al.Progress in the study of functional groups and health care efficacy of Ginkgo biloba leaves[J].Food Research and Development,2020,41(15):182-187.
[3] 姜爱丽,孟宪军,胡文忠,等.不同北高丛蓝莓品种的抗氧化成分及其抗氧化活性[J].食品与发酵工业,2011,37(9):161-165.
JIANG A L,MENG X J,HU Z W,et al.Study on the biological active substances and antioxidant capacity in various cultivars of northern highbush blueberry fruits[J].Food and Fermentation Industries,2020,41(15):182-187.
[4] 符群,王梦丽,李娜,等.暖木条荚蒾果抗氧化成分的制备及主要活性成分分析[J].中南林业科技大学学报,2019,39(12):114-122.
FU Q,WANG M L,LI N,et al.Extraction of antioxidant components from the fruits of Viburnum and analysis of its main active ingredient[J].Journal of Central South University of Forestry & Technology,2019,39(12):114-122.
[5] 何佳林,李潇雨,李秀红,等.不同银杏无性系叶片萜内酯含量的差异性研究[J].浙江林业科技,2020,40(2):30-35.
HE J L,LI X Y,LI X H,et al.Experiment on difference of terpene lactones content in leaves of 20 Ginkgo biloba clones[J].Journal of Zhejiang Forestry Science and Technology,2020,40(2):30-35.
[6] 韩晋,郁万文,曹福亮,等.基于叶和花粉中类黄酮含量的银杏雄性优株初选[J].经济林研究,2019,37(4):88-95.
HAN J,YU W W,CAO F L,et al.Preliminary selection of superior male Ginkgo biloba plants based on flavonoid content in leaves and pollen[J].Non-Wood Forest Research,2019,37(4):88-95.
[7] 吴岐奎,邢世岩,王萱,等.叶用银杏种质资源黄酮和萜内酯类含量及AFLP遗传多样性分析[J].园艺学报,2014,41(12):2 373-2 382.
WU Q K,XING S Y,WANG X,et al.Genetic diversity of leaf-used Ginkgo biloba germplasms based on flavonoids and ginkgolides contents and AFLP markers[J].Acta Horticultural Sinica,2014,41(12):2 373-2 382.
[8] 郁万文,刘新亮,曹福亮,等.不同银杏无性系叶药用成分差异及聚类分析[J].植物学报,2014,49(3):292-305.
YU W W,LIU X L,CAO F L,et al.Cluster analysis on the main medicinal components in differential leaves of ginkgo clones[J].Chinese Bulletin of Botany,2014,49(3):292-305.
[9] 李卫星,甄珍,周春华,等.银杏雄株叶片和花粉主要类黄酮成分含量分析[J].中国农业科学,2010,43(13):2 775-2 783.
LI W X,ZHEN Z,ZHOU C H,et al.Flavonoid content of the leaves and pollen of male Ginkgo biloba plants[J].Scientia Agricultura Sinica,2010,43(13):2 775-2 783.
[10] 邢世岩,吴德军,邢黎峰,等.银杏叶药物成分的数量遗传分析及多性状选择[J].遗传学报,2002,29(10):928-935.
XING S Y,WU D J,XING L F,et al.Quantitative genetic analysis and multiple trait selection of pharmaceutical composition on Ginkgo biloba L.leaves[J].Acta Genetica Sinica,2002,29(10):928-935.
[11] 姚鑫.不同来源银杏叶资源化学研究[D].南京:南京中医药大学,2013.
YAO X.Study on the resource chemistry of Ginkgo biloba leaves from different origin in China[D].Nanjing:Nanjing University of Chinese Medicine,2013.
[12] 张曦元.银杏黄酮的制备及其抗氧化性研究[D].大连:大连工业大学,2016.
ZHANG X Y.Preparation of Ginkgo flavonoids and study on its antioxidant activities[D].Dalian:Dalian Polytechnic University,2016.
[13] 张渝阳,王鑫,赵丽娟,等.分光光度法测定银杏萜内酯的含量[J].辽宁化工,2006,35(10):618-620.
ZHANG Y Y,WANG X,ZHAO L J,et al.Spectrophotometric determination of total terpenoids in ginkgo leaves[J].Liaoning Chemical Industry,2006,35(10):618-620.
[14] 乔洪翔,任红,陈君,等.银杏叶制剂中原花青素含量测定方法比较[J].海峡药学,2017,29(8):61-63.
QIAO H X,REN H,CHEN J.et al.Comparison of determination methods of proanthocyanidins in Ginkgo biloba preparations[J].Strait Pharmaceutical Journal,2017,29(8):61-63.
[15] 吴雅琼,国靖,周琦,等.不同产地银杏黄酮及相关活性物质含量变异分析[J].南京林业大学学报(自然科学版),2019,43(3):183-188.
WU Y Q,GUO J,ZHOU Q,et al.Variation analysis of flavonoids and related active substances content in ginkgo leaves from different regions[J].Journal of Nanjing Forestry University(Natural Sciences Edition),2019,43(3):183-188.
[16] 于国栋,夏米蕊,杨新成,等.银杏古树不同产地和家系子代生长及叶片药用成分含量的差异[J].北方园艺,2018,(11):144-150.
YU G D,XIA M R,YANG X C,et al.Growth and medicinal component contents of leaves for different families’ progenies originated in different seed sources of ancient Ginkgo biloba[J].Northern Horticulture,2018,(11):144-150.
[17] 辜夕容,江亚男,倪亚兰,等.叶用银杏的良种选育与定向培育研究进展[J].中草药,2017,48(15):3 218-3 227.
GU X R,JIANG Y N,NI Y L,et al.Research progress on breeding and cultivation of Ginkgo biloba for leaf use in China[J].Chinese Traditional and Herbal Drugs,2017,48(15):3 218-3 227.
[18] 刘芳瑞,胡晶红,李佳,等.银杏植株活性物质合成与积累的影响因素分析[J].山东中医药大学学报,2011,35(3):199-202.
LIU F R,HU J H,LI J,et al.Discussion on synthesis and accumulation factors of active substance of Ginkgo biloba[J].Journal of Shandong University of Traditional Chinese Medicine,2011,35(3):199-202.
[19] 李诚勇.修剪对叶用银杏黄酮和内酯类物质含量的影响[J].山东林业科技,2009,39(5):71-72.
LI C Y.Effects of pruning on the contents of flavonoids and lactones in Ginkgo biloba[J].Shandong Forestry Science and Technology,2009,39(5):71-72.
[20] 陈华圣,任莉,许爱华,等.不同龄期性别银杏叶多糖含量的比较研究[J].中药材,2006,29(11):1 139-1 141.
CHEN H S,REN L,XU A H,et al.Study on the comparison of polysaccharides in Ginkgo biloba leaves[J].Journal of Chinese Medicinal Materials,2006,29(11):1 139-1 141.
[21] 王晓红,孙延龙,李万忠,等.产地和生长年限对银杏叶中多糖和黄酮含量的影响[J].中药材,2016,39(6):1 341-1 342.
WANG X H,SUN Y L,LI W Z,et al.Effects of origin and growth years on the contents of polysaccharides and flavonoids in Ginkgo biloba leaves[J].Journal of Chinese Medicinal Materials,2016,39(6):1 341-1 342.
[22] 李无双,周燕.银杏叶提取物抗氧化作用的研究进展[J].世界最新医学信息文摘,2017,17(26):37-38.
LI W S,ZHOU Y.The research progress of Ginkgo biloba extract antioxidant effect[J].World Latest Medicine Information,2017,17(26):37-38.
[23] 张媛华,权美平.银杏叶提取物抗氧化功效的研究进展[J].黑龙江畜牧兽医,2016(11):69-71.
ZHANG Y H,QUAN M P.Research progress on antioxidant effect of Ginkgo biloba extract[J].Heilongjiang Animal Science and Veterinary Medicine,2016(11):69-71.
[24] 宋唯唯,刘芬芬,刘彩红.银杏叶提取物与其黄酮类物质抗氧化作用的比较[J].泰山医学院学报,2016,37(5):484-486.
SONG W W,LIU F F,LIU C H.Comparison of the antioxidant activity between Gingko biloba extract and its total flavonoids[J].Journal of Taishan Medical College,2016,37(5):484-486.
[25] LIU X P,LUAN J J,CHRISTOPHER E P G.Comparison of the antioxidant activity amongst Gingko biloba extract and its main components[J].Journal of Chinese Medicinal Material,2009,32(5):736-740.
[26] 牟玲丽,寇俊萍,朱丹妮,等.银杏叶的化学成分及其抗氧化活性[J].中国天然药物,2008,6(1):26-29.
MU L L,KOU J P,ZHU D N,et al.Antioxidant activities of the chemical constituents isolated from the leaves of Ginkgo biloba[J].Chinese Journal of Natural Medicines,2008,6(1):26-29.
[27] 黄海波,刘华春,金朱明,等.银杏叶原花青素的制备及其抗氧化活性研究[J].中国现代应用药学,2016,33(6):686-690.
HUANG H B,LIU H C,JIN Z M,et al.Proanthocyanidins from Ginkgo biloba L.and their anti-oxidant activities[J].Chinese Journal of Modern Applied Pharmacy,2016,33(6):686-690.
[28] 何钢,刘嵬,李会萍,等.银杏叶多糖分离纯化、结构鉴定及抗氧化活性研究[J].食品工业科技,2015,36(22):81-86.
HE G,LIU W,LI H P,et al.Isolation and purification,structure identification and antioxidant activity of polysaccharides of Ginkgo biloba leaf[J].Science and Technology of Food Industry,2015,36(22):81-86.
[29] 任佳伟,宋玉.银杏叶有效成分抗血小板聚集和清除DPPH自由基的量效关系和协同作用[J].世界科学技术-中医药现代化,2015,17(11):2 367-2 372.
REN J W,SONG Y.Dose-response relation and synergy effects of bioactive components in Ginkgo biloba on antiplatelet aggregation and DPPH free radical scavenging[J].Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology,2015,17(11):2 367-2 372.
[30] 韩京艳,邢东明,孙虹,等.银杏叶黄酮内酯及天然配比抗氧化作用的比较[J].中国药理学通报,2002,18(1):115-117.
HAN J Y,XING D M,SUN H,et al.Comparison of flavonoids,terpenoids and the natural complex of them by Ginkgo biloba on antioxidant effect[J].Chinese Pharmacological Bulletin,2002,18(1):115-117.
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