Food and Fermentation Industries >

2022 , Vol. 48 >Issue 5: 157 - 164

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027943

The differentiation of total polyphenols and total flavonoids contents in Gastrodia elata Bl.f.glauca S.Chow extracts using different solvents and their antioxidant capacities

  • LIAO Xia ,
  • WU Zhen ,
  • YANG Yong ,
  • CHEN Gang ,
  • ZHAN Yong
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  • 1(Chongqing Academy of Chinese Materia Medica,Chongqing Key Laboratory of Chinese Medicine and Health Science,Chongqing 400065,China)
    2(Chongqing Engineering Technology Research Center of Materia Medica Health,Chongqing 400065,China)

Online published: 2022-04-06

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Abstract

Gastrodia elata has been included in the pilot project of herb-food homology, and is commonly used in stews, dishes and hot pot in the southwest of China. In order to evaluate the differences of main active components and antioxidant activities in the processing and application of edible Gastrodia elata Bl, the contents of total polyphenols and total flavonoids of Yunyang G.elata Bl.f.glauca S. Chow extracts with five different solvents were determined, and the composition of polyphenols was analyzed by HPLC. Meanwhile, the antioxidant activities were also evaluated by DPPH and ABTS cationic free radical scavenging capacities. The results showed that the extract with 70% (volume fraction) methanol showed the highest content of total polyphenols and total flavonoids, which were 4.006 mg GAE/g and 1.290 mg RE/g, respectively. Eight polyphenols and their glycosides were identified by HPLC, and gastrodin was the highest in 70% (volume fraction) ethanol extract. Furthermore, the contents of 4-hydroxybenzaldehyde (0.014 mg/g), Parishin B (1.745 mg/g), Parishin C (2.255 mg/g) and Parishin E (4.181 mg/g) in 70% (volume fraction) methanol extract were the highest. The water extract had the strongest scavenging ability on DPPH free radical, while the methanol extract had the best scavenging effect on ABTS cationic radical, and there was a high correlation between their antioxidant capacity and the contents of active components. The methanol extract of G. elata Bl. f. glauca S. Chow contains the highest amount of active components and possesses the highest antioxidant activity, which can be used as a potential source of natural antioxidants.

Key words： Gastrodia elata Bl. f. glauca S. Chow; total polyphenols; total flavonoids; antioxidant capacity; correlation

Cite this article

LIAO Xia , WU Zhen , YANG Yong , CHEN Gang , ZHAN Yong . The differentiation of total polyphenols and total flavonoids contents in Gastrodia elata Bl.f.glauca S.Chow extracts using different solvents and their antioxidant capacities[J]. Food and Fermentation Industries, 2022 , 48(5) : 157 -164 . DOI: 10.13995/j.cnki.11-1802/ts.027943

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