Food and Fermentation Industries >

2022 , Vol. 48 >Issue 6: 233 - 240

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027899

Cluster analysis on amino acid properties of six livestock milk

  • YANG Huiru ,
  • GUO Jun ,
  • Gusilengtu ,
  • WANG Yuenan ,
  • ZHANG Chunhua ,
  • SUN Haizhou
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  • 1(College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China)
    2(Institute of Animal Nutrition and Feed, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China)

Received date: 2021-05-04

  Revised date: 2021-05-31

  Online published: 2022-04-25

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Abstract

A total of 55 representative milk samples of Holstein cow(cow), Mongolian horse(horse), Bactrian camel, goat, buffalo and yak were collected for the determination of 18 amino acids(AA) by traditional descriptive statistics, nutritional evaluation and cluster analysis. The results showed that the amino acids absolute content (mg/g) were significantly different (P<0.01). Total amino acid(TAA)of the six animal milk were 1.7-5.4 times higher than that of mix human milk released from Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO). And in which, TAA of yak and buffalo were the highest, followed by the goat, camel, cow and horse milk. Principal component analysis(PCA) on percentage data of AA (AA/TAA, %) showed that clustering of six livestock milk samples in 3D space conformed to taxonomy class of the animal’s species, four Bovidae animal milk samples clustered in the same region, while the cluster of horse and camel milk samples were far in another two regions. Human milk was close to horse milk. Trp, Cys, Asp and Arg in human milk were relatively insufficient in livestock milk. The amount of Trp in Goat was close to human milk. Essential amino acid (EAA) pattern of livestock milk was significantly different from human milk (P<0.01). PCA showed that the milk of six livestock was relatively together, while the human milk was far away from them, which implied that the EAA pattern of human milk was significantly different from that of animals. Goat and horse milk were relatively close to human milk according to their EAA pattern. Moreover, soft independent modeling of class analogies species discrimination model was established based on milk AA% data, and the internal and external verification accuracy rates were 100% and 92.76% respectively. External validation errors appeared within the Bovidae milk samples. Human milk cannot be classified into any one of the six animals which indicating that the amino acids of human milk and animals were different. Chemometrics cluster analysis is more visual and holographic than traditional descriptive statistics and protein amino acids nutritional evaluation. This study can provide innovative and creative strategies, principles and methods for the evaluation of nutrition and authenticity of dairy products.

Key words： livestock milk; amino acids; principal component analysis; soft independent modeling of class analogies; authenticity; human milk

Cite this article

YANG Huiru , GUO Jun , Gusilengtu , WANG Yuenan , ZHANG Chunhua , SUN Haizhou . Cluster analysis on amino acid properties of six livestock milk[J]. Food and Fermentation Industries, 2022 , 48(6) : 233 -240 . DOI: 10.13995/j.cnki.11-1802/ts.027899

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