Food and Fermentation Industries >

2022 , Vol. 48 >Issue 6: 263 - 269

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.030123

Study on high-performance thin-layer chromatography analysis and antioxidant ability of Hippophae rhamnoides L. fruits

  • GE Liang ,
  • LI Na ,
  • YANG Minghan ,
  • TIAN Shuge
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  • (College of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi 830011, China)

Received date: 2021-11-17

  Revised date: 2021-11-26

  Online published: 2022-04-25

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Abstract

A high-performance thin-layer chromatography (HPTLC) method was established for the qualitative and quantitative analysis of malic acid and quinic acid in Hippophae rhamnoides L. fruits, and an ultraviolet method was established to determine the clearance rate of Hippophae rhamnoides L. fruits to DPPH free radicals, ABTS cationic free radicals and superoxide anion radicals(·O-2). Took V(dichloromethane)∶V (ethyl acetate)∶V (formic acid) =5∶6∶2.5 as the developing reagent, the CamagTLC3 scanner was used to determine the content of malic acid and quinic acid in Hippophae rhamnoides L. fruits from 10 different producing areas. The antioxidant ability of Hippophae rhamnoides L. fruits was evaluated by DPPH method, ABTS method, ·O-2 method. The qualitative and quantitative analysis results showed that the Rf value of malic acid was 0.59±0.05, and the Rf value of quinic acid was 0.45±0.05. The methodological investigation results showed that the analytical method of HPTLC was good. Furthermore, the antioxidant results showed that within the test concentration range, the IC50 of S7-S10 Hippophae rhamnoides L. fruits extract DPPH free radicals was 0.11-0.13 mg/mL and the maximum clearance rate was 93.71%-96.27%. The IC50 of ABTS cationic free radicals was 0.11-0.61 mg/mL and the maximum clearance rate was 92.18%-99.39%. And the IC50 of ·O-2 was 1.80-2.90 mg/mL and the maximum clearance rate was 81.92%-100.07%. The clearance rates of DPPH free radicals, ABTS cationic free radicals, and·O-2 were all less than vitamin C. It was showed that the Hippophae rhamnoides L. fruit extract had a good dose-dependent DPPH free radical, ABTS cationic free radicals and ·O-2 antioxidation ability within a certain concentration range. The method established was simple and could be widely promote to the rapid detection of Hippophae rhamnoides L. fruits and provided a certain reference for the quality control of Hippophae rhamnoides L. fruits. This study showed that Hippophae rhamnoides L. fruits was medicinal and food plants with high antioxidant properties.

Key words： Hippophae rhamnoides L. fruits; malic acid; quinic acid; high-performance thin-layer chromatography; DPPH free radicals; ABTS cationic free radicals; superoxide anion radicals

Cite this article

GE Liang , LI Na , YANG Minghan , TIAN Shuge . Study on high-performance thin-layer chromatography analysis and antioxidant ability of Hippophae rhamnoides L. fruits[J]. Food and Fermentation Industries, 2022 , 48(6) : 263 -269 . DOI: 10.13995/j.cnki.11-1802/ts.030123

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