Food and Fermentation Industries >

2022 , Vol. 48 >Issue 6: 276 - 280

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026945

Determination of avermectin residues in four kinds of fruits and vegetables by LC-APCI-MS-MS

  • ZENG Dingling ,
  • FANG Changfa ,
  • GU Yaping ,
  • PAN Jianlei ,
  • LIN Huichun ,
  • ZHANG Xiaoqiang ,
  • ZHANG Xiaohong ,
  • WANG Yuxin
Expand
  • 1(Shenzhen Centre of Inspection and Testing for Agricultural Products, Shenzhen 518040, China)
    2(School of Applied Chemistry and Biological Technology, Shenzhen Polytechnic, Shenzhen 518055, China)

Received date: 2021-02-07

  Revised date: 2021-06-18

  Online published: 2022-04-25

Fold

Abstract

A method for the determination of avermectin residues in fruits and vegetables was established in this study. Four matrices were treated with modified QuEChERS and liquid chromatography-tandem mass spectrometry (LC-MS-MS) with atmospheric pressure chemical ionization (APCI). The stability, matrix effect and method accuracy of the method was ed. The stability in 5 hours of the standard solution was below 5% tested every 1 hour. The results showed that correlation coefficients of standard curves in four matrices were greater than 0.99 in the linear range of 0.012 5-0.40 mg/L; the matrix effect on the APCI mode was between 0.97 and 1.05. The spiked recovery results of avermectin in four matrices were 93.3% and 100.8% with the RSD of 1.8% to 5.7% (when in the adding amount of 0.01, 0.1 and 0.2 mg/kg). The proposed method was more suitable for the quantitative analysis of avermectin in agricultural products for its stability, convenience, accuracy and less matrix effect.

Key words： avermectin; pesticides; atmospheric pressure chemical ionization; QuEChERS

Cite this article

ZENG Dingling , FANG Changfa , GU Yaping , PAN Jianlei , LIN Huichun , ZHANG Xiaoqiang , ZHANG Xiaohong , WANG Yuxin . Determination of avermectin residues in four kinds of fruits and vegetables by LC-APCI-MS-MS[J]. Food and Fermentation Industries, 2022 , 48(6) : 276 -280 . DOI: 10.13995/j.cnki.11-1802/ts.026945

References

[1] 文豪, 周绪正, 李冰, 等.阿维菌素类药物残留检测的研究进展[J].黑龙江畜牧兽医, 2015(21):55-57.
WEN H, ZHOU X Z, LI B, et al.Research progress on the detection of the residue of avermectins[J].Heilongjiang Animal Science and Veterinary Medicine, 2015(21):55-57.
[2] 刘晓艳, 闵勇, 饶犇, 等.杀螨剂研究进展[J].生物资源, 2019, 41(4):305-313.
LIU X Y, MIN Y, RAO B, et al.Research advances on acaricides[J].Biotic Resources, 2019, 41(4):305-313.
[3] SU W K, HOU Z N, LIANG X R.Separation of avermectin components from Streptomyces avemitilis extraction using high-speed counter-current chromatography[J].Chemical Industry & Chemical Engineering Quarterly, 2014, 19(4):563-571.
[4] WANG S Y, BO Y H, ZHOU X, et al.Significance of heavy-ion beam irradiation-induced avermectin B1a production by engineered Streptomyces avermitilis[J].BioMed Research International, 2017, 2017:5373262.
[5] 覃国新, 劳水兵, 莫仁甫, 等.超高效液相色谱法测定甘蓝和橙子中的阿维菌素残留[J].现代食品科技, 2018, 34(4):227-231.
QIN G X, LAO S B, MO R F, et al.Determination of abamectin residues in cabbage and orange by ultra performance liquid chromatography[J].Modern Food Science and Technology, 2018, 34(4):227-231.
[6] HUANG Y, HONG Y H, HUANG Z Q, et al.Avermectin induces the oxidative stress, genotoxicity, and immunological responses in the Chinese Mitten Crab, Eriocheir sinensis[J].PLoS One, 2019, 14(11):e0225171.
[7] 中华人民共和国国家卫生健康委员会, 农业农村部, 国家市场监督管理总局.GB 2763—2019 食品安全国家标准食品中农药最大残留限量[S].北京:中国标准出版社, 2019.
National Health Commission of the People's Republic of China, Ministry of Agriculture and Rural Affairs of the People's Republic of China, State Administration for Market Regulation.GB 2763—2019 National food safety standard Maximum resudus limits for pesticides in food[S].Beijing:China Standards Press, 2019.
[8] 农业农村部. NY/T 1379—2007 蔬菜中334种农药多残留的测定气相色谱质谱法和液相色谱质谱法[S].北京:中国标准出版社, 2007.
Ministry of Agriculture and Rural Affairs of the People's Republic of China.NY/T 1379—2007 Multi-residue determination of 334 pesticides in vegetable by GC/MS and LC/MS[S].Beijing:China Standards Press, 2007.
[9] 国家卫生和计划生育委员会, 农业部, 国家食品药品监督管理总局.GB 23200.20—2016 食品安全国家标准食品中阿维菌素残留量的测定液相色谱-质谱/质谱法[S].北京:中国标准出版社, 2016.
National Health Commission of the People's Republic of China, Ministry of Agriculture and Rural Affairs of the People's Republic of China, State Administration for Market Regulation.GB 23200.20—2016 National food safety standard Determination of abamectin residue in foods liquid chromatography-mass spectrometry[S].Beijing:China Standards Press, 2016.
[10] 国家卫生和计划生育委员会, 农业农村部, 国家食品药品监督管理总局.GB 23200.19—2016 食品安全国家标准水果和蔬菜中阿维菌素残留量的测定液相色谱法[S].北京:中国标准出版社, 2016.
National Health Commission of the People's Republic of China, Ministry of Agriculture and Rural Affairs of the People's Republic of China, State Administration for Market Regulation.GB 23200.19—2016 National food safety standard Determination of abamectin residue in fruits and vegetables liquid chromatography[S].Beijing:China Standards Press, 2016.
[11] 熊壮. HPLC法检测蔬菜中的阿维菌素[J].山东化工, 2017, 46(12):92-93.
XIONG Z.HPLC determination method for the avermectin content in the vegetable samples[J].Shandong Chemical Industry, 2017, 46(12):92-93.
[12] 张元, 谷岩, 贾宏新.固相萃取-高效液相色谱-紫外检测法测定蔬菜中阿维菌素的残留量[J].食品安全质量检测学报, 2020, 11(6):1 958-1 961.
ZHANG Y, GU Y, JIA H X.Determination of avermectin residue in vegetables by solid-phase extraction with ultraviolet detection coupled to high performance liquid chromatography[J].Journal of Food Safety & Quality, 2020, 11(6):1 958-1 961.
[13] 张儒令, 安凤颖, 胡德禹, 等.高效液相色谱法检测菜豆中阿维菌素残留量[J].现代农业科技, 2020(6):106-108.
ZHANG R L, AN F Y, HU D Y, et al.Determination of abamectin residue in kidney bean by HPLC[J].Modern Agricultural Science and Technology, 2020(6):106-108.
[14] 吴添虎. 高效液相色谱荧光法同时检测黄瓜及土壤中的阿维菌素和甲维盐[J].广东化工, 2016, 43(1):120-121;100.
WU T H.Multi-residues determination of abamectin and emamectin benzoate in cucumber and soil by HPLC-FLD[J].Guangdong Chemical Industry, 2016,43(1):120-121;100.
[15] 石红霞, 魏万彩, 雒淑雯, 等.液相色谱-串联三重四级杆质谱法测定果蔬中的阿维菌素[J].湖北农业科学, 2020, 59(S1):441-443.
SHI H X, WEI W C, LUO S W, et al.Determination of avermectin residues in vegetables andfruits by QuEChERS-LC/MS/MS[J].Hubei Agricultural Sciences, 2020, 59(S1):441-443.
[16] 方玲, 卞红正, 赵宏梅.QuEChERS-UPLC-MS/MS法测定水果蔬菜中的阿维菌素[J].安徽农学通报, 2019, 25(9):106-108.
FANG L, BIAN H Z, ZHAO H M.QuEChERS-UPLC-MS/MS method for determination of abamectin pesticide residues in vegetables and fruits[J].Anhui Agricultural Science Bulletin, 2019, 25(9):106-108.
[17] 李二虎, 吴兵兵, 谢蕴琳, 等.串联液质测定小白菜和土壤中阿维菌素的残留量[J].农药科学与管理, 2017, 38(2):30-34.
LI E H, WU B B, XIE Y L, et al.Determination of abramectin residue in pakchoi and soil by HPLC-MS/MS[J].Pesticide Science and Administration, 2017, 38(2):30-34.
[18] 林志惠, 李慧珍, 游静.高效液相色谱-串联质谱法检测水体沉积物中阿维菌素的残留量[J].分析测试学报, 2013, 32(8):923-928.
LIN Z H, LI H Z, YOU J.Determination of abamectin residue in sediment using high performance liquid chromatography-tandem mass spectrometry[J].Journal of Instrumental Analysis, 2013, 32(8):923-928.
[19] MATUSZEWSKI B K, CONSTANZER M L, CHAVEZ-ENG C M.Strategies for the assessment of matrix effect in quantitative bioanalytical methods based on HPLC-MS/MS[J].Analytical Chemistry, 2003, 75(13):3 019-3 030.
[20] 顾亚萍, 乔方, 林慧纯, 等.4种蔬菜中20种农药残留的基质效应的研究[J].食品研究与开发, 2017, 38(22):148-152.
GU Y P, QIAO F, LIN H C, et al.Research of matrix effect of 20 pesticide residue in 4 kinds of vegetables[J].Food Research and Development, 2017, 38(22):148-152.
[21] 张伟彪, 顾亚萍, 曾定玲, 等.三种常用溶剂对98种农残检测结果稳定性的影响[J].食品与发酵工业, 2020, 46(16):236-242.
ZHANG W B, GU Y P, ZENG D L, et al.Study on the stability of 98 pesticide standards in three common solvents[J].Food and Fermentation Industries, 2020, 46(16):236-242.
[22] FACORRO R, LLOMPART M, DAGNAC T.Combined (d) SPE-QuEChERS extraction of mycotoxins in mixed feed rations and analysis by high performance liquid chromatography-high-resolution mass spectrometry[J].Toxins, 2020, 12(3):206.
[23] 何红梅, 赵华, 张春荣, 等.超高效液相色谱-串联质谱法测定水稻基质中阿维菌素残留量[J].分析化学, 2012, 40(1):140-144.
HE H M, ZHAO H, ZHANG C R, et al.Determination of abamectin residue in paddy rice by ultra performance liquid chromatography-tandem mass spectrometry[J].Chinese Journal of Analytical Chemistry, 2012, 40(1):140-144.
[24] SONG N E, YOO M, NAM T G.Multi-residue analysis of 203 pesticides in strawberries by liquid chromatography tandem mass spectrometry in combination with the QuEChERS method[J].CyTA - Journal of Food, 2019, 17(1):976-987.
[25] 黄兰淇, 陈秀, 张正炜, 等.超高效液相色谱-串联质谱法同时检测大米中15种常用农药的残留[J].农药学学报, 2018, 20(3):354-362.
HUANG L Q, CHEN X, ZHANG Z W, et al.Determination of 15 pesticides residues in rice by ultra performance liquid chromatography-tandem mass spectrometry[J].Chinese Journal of Pesticide Science, 2018, 20(3):354-362.
[26] FRENICH A G, ROMERO-GONZÁLEZ R, GÓMEZ-PÉREZ M L, et al.Multi-mycotoxin analysis in eggs using a QuEChERS-based extraction procedure and ultra-high-pressure liquid chromatography coupled to triple quadrupole mass spectrometry[J].Journal of Chromatography A, 2011, 1 218(28):4 349-4 356.
[27] 国家质量监督检验检疫总局, 中国国家标准化管理委员会.GB/T 27404—2008 实验室质量控制规范 食品理化检测[S].北京:中国标准出版社, 2008.
State Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, China National Standardization Administration Committee.GB/T 27404—2008 Criterion on quality control of laboratories Chemical testing of food[S].Beijing:China Standards Press, 2008.
[28] 国家质量监督检验检疫总局, 中国国家标准化管理委员会.GB/T 27417—2017 合格评定 化学分析方法确认和验证指南[S].北京:中国标准出版社, 2008.
State Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, China National Standardization Administration Committee.GB/T 27417—2017 Comformity assessment—Guidance on validationand verification of chemical analytical methods[S].Beijing:China Standards Press, 2017.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.