Food and Fermentation Industries >

2022 , Vol. 48 >Issue 8: 1 - 8

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028784

Protective effect of exopolysaccharide from Lactobacillus fermentum LFQ153 against oxidative damage in RAW264.7 macrophages

  • LIU Congxiu ,
  • SONG Jiajia ,
  • WANG Hongwei ,
  • ZHANG Yu ,
  • SUO Huayi
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  • (College of Food Science, Southwest University, Chongqing 400715, China)

Received date: 2021-07-26

  Revised date: 2021-08-26

  Online published: 2022-05-18

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Abstract

In order to investigate the protective effects and underlying the mechanisms of exopolysaccharides (EPS) from Lactobacillus fermentum LFQ153 against oxidative damage in RAW264.7 macrophages, the current study evaluated the in vitro antioxidant activity of EPS using the DPPH and ·OH radical scavenging ability, and established the cell model of oxidative damage by stimulating RAW264.7 macrophages with lipopolysaccharide (LPS). The protective effects of EPS against cell oxidative damage were investigated based on the activity of antioxidant enzymes, the content of malondialdehyde (MDA) and NO, and the expression of inflammatory cytokines. The expression of nuclear factor E2-related factor 2/heme oxygenase-1/nuclear factor-κB (Nrf2/HO-1/NF-κB) pathway-related genes was analyzed to explore the protective mechanisms of EPS against cell oxidative damage. In addition, the monosaccharide composition of EPS was assayed by ion chromatography in the present study. The DPPH and ·OH radical scavenging rates of EPS were (64.19±1.03)% and (61.87±3.09)%, respectively. Compared with LPS treatment group, EPS supplement significantly increased the activities of superoxide dismutase, catalase and glutathione peroxidase in macrophages, and decreased the contents of MDA and NO, as well as the mRNA expression levels of nitric oxide synthase, tumor necrosis factor-α, interleukin (IL)-1β and IL-6. Moreover, EPS promoted the mRNA expression levels of Nrf2 and HO-1, and inhibited the activation of NF-κB in macrophages with oxidative damage. In addition, the structural analysis showed that EPS was composed of glucosamine hydrochloride, galactose, glucose and mannose with the molar ratio of 2.48∶10.26∶50.22∶8.16. Our study indicates that EPS may protect macrophages against oxidative damage, and this protective effect is associated with the regulation of Nrf2/HO-1/NF-κB pathway and the monosaccharide composition of EPS.

Key words： Lactobacillus fermentum; exopolysaccharide; macrophages; oxidative damage

Cite this article

LIU Congxiu , SONG Jiajia , WANG Hongwei , ZHANG Yu , SUO Huayi . Protective effect of exopolysaccharide from Lactobacillus fermentum LFQ153 against oxidative damage in RAW264.7 macrophages[J]. Food and Fermentation Industries, 2022 , 48(8) : 1 -8 . DOI: 10.13995/j.cnki.11-1802/ts.028784

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