Correlation analysis between starch and glucose content in rice wine brewing

  • HE Yulin ,
  • YUAN Guoyi ,
  • WANG Chunxiao ,
  • ZHOU Wenya ,
  • QIU Shuyi
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  • (Province Key Laboratory of Fermentation Engineering and Biopharmacy, School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China)

Received date: 2021-07-27

  Revised date: 2021-09-05

  Online published: 2022-05-18

Abstract

Raw materials are closely related to the flavor formation of rice wine. In order to analyze the difference of starch content among rice varieties, and its correlation with glucose content in rice wine, this study chose different rice varieties to simulate rice wine fermentation in the laboratory. Double wavelength method was used to detect the starch content in different rice varieties, and 3,5-dinitrosalicylic acid (DNS) colorimetric method was used to determine the glucose content at different fermentation time points. The results showed that the content of amylopectin and total starch in Baishui Gongmi (BS) was the highest among the ten rice varieties, which were (83.59±2.70)% and (90.68±2.88)%, respectively. During the fermentation of rice wine, Saccharomyces cerevisiae gradually metabolized glucose to other substances, resulting in a downward trend of glucose content at the zero point (0), midpoint (1) and end point (2) of fermentation. The glucose consumption was the highest during the whole fermentation of rice wine using the Wuchang glutinous rice (WC), which was 453.15 g/L. Correlation analysis showed that a negative correlation was found between the glucose consumption during fermentation and the starch content in rice. The glucose consumption at the late stage of fermentation and the total glucose consumption were significantly negative correlated (P<0.01) with the amylopectin and total starch content in rice. The correlation coefficients between glucose consumption at the late stage, total glucose consumption, and the rice varieties reached 0.761 and 0.506, respectively, which were extremely significant (P<0.01). In summary, the glucose consumption during rice wine fermentation is significantly related to rice varieties. This study provides an important basis for the selection of rice wine varieties and the improvement of rice wine quality.

Cite this article

HE Yulin , YUAN Guoyi , WANG Chunxiao , ZHOU Wenya , QIU Shuyi . Correlation analysis between starch and glucose content in rice wine brewing[J]. Food and Fermentation Industries, 2022 , 48(8) : 28 -36 . DOI: 10.13995/j.cnki.11-1802/ts.028723

References

[1] WEI X L, LIU S P, YU J S, et al.Innovation Chinese rice wine brewing technology by bi-acidification to exclude rice soaking process[J].Journal of Bioscience and Bioengineering, 2017, 123(4):460-465.
[2] MENG L P, LIN H, PAN S L, et al.Chinese rice wine polyphenol compounds inhibit vascular smooth muscle cell dedifferentiation and its mechanism[J].International Journal of Clinical and Experimental Medicine, 2016, 9(9):17 539-17 548.
[3] CHEN T, WU F H, GUO J J, et al.Effects of glutinous rice protein components on the volatile substances and sensory properties of Chinese rice wine[J].Journal of the Science of Food & Agriculture, 2020, 100(8):3 297-3 307.
[4] 张高楠, 苏钰亭, 赵思明, 等.4种甜米酒主要营养成分与滋味特征对比及分析[J].华中农业大学学报, 2018, 37(2):89-95.
ZHANG G N, SU Y T, ZHAO S M, et al.Analyses of nutritional composition in four kinds of fermented sweet rice wines and sensory evaluation with electronic tongue[J].Journal of Huazhong Agricultural University, 2018, 37(2):89-95.
[5] LAI Q, LI Y H, WU Y W, et al.The quality of rice wine influenced by the crystal structure of rice starch[J].Journal of Food Science and Technology-Mysore, 2019, 56(4):1 988-1 996.
[6] 程度, 邱树毅.高粱对酱香型白酒品质影响研究进展[J/OL].食品科学, 2021.https://kns.cnki.net/kcms/detail/11.2206.TS.20210406.1527.055.html.
CHEN D, QIU S Y.Research progress on the effect of sorghum on the quality of Maotai-flavor liquor[J/OL].Food Science, 2021.https://kns.cnki.net/kcms/detail/11.2206.TS.20210406.1527.055.html.
[7] 袁国亿, 王春晓, 何宇淋, 等.酶标仪比色法检测浊米酒中高级醇含量的研究[J].食品与发酵工业, 2021, 47(6):221-227.
YUAN G Y, WANG C X, HE Y L, et al.Detection of higher alcohols content in turbid rice wine by the colorimetric method of microplate reader[J].Food and Fermentation Industries, 2021, 47(6):221-227.
[8] HUANG G D, REN H, WANG A L, et al.iTRAQ-based proteomic analysis reveals the molecule mechanism of reducing higher alcohols in Chinese rice wine by nitrogen compensation[J].Annals of Microbiology, 2021, 71(1):1-13.
[9] LI W, WANG J H, ZHANG C Y, et al.Regulation of Saccharomyces cerevisiae genetic engineering on the production of acetate esters and higher alcohols during Chinese Baijiu fermentation[J].Journal of Industrial Microbiology & Biotechnology, 2017, 44(6):949-960.
[10] DONG J, XU H Y, ZHAO L B, et al.Enhanced acetate ester production of Chinese liquor yeast by overexpressing ATF1 through precise and seamless insertion of PGK1 promoter[J].Journal of Industrial Microbiology & Biotechnology, 2014, 41(12):1 823-1 828.
[11] 邹凌波, 王栋, 余海燕, 等.基于理化指标和风味成分的酿造米酒分类及其相关特性分析[J].食品与发酵工业, 2019, 45(13):78-84.
ZOU L B, WANG D, YU H Y, et al.Classification and characteristics of typical fermented Mijiu based on physicochemical parameters and flavor components[J].Food and Fermentation Industries, 2019, 45(13):78-84.
[12] WU Q, CAO S, XU Y.Effects of glutinous and nonglutinous sorghums on Saccharomyces cerevisiae fermentation for Chinese liquor making[J].International Journal of Food Science & Technology, 2017, 52(6):1 348-1 357.
[13] 唐佳代. 贵州传统小曲微生物群落结构及功能酵母的筛选[D].贵阳:贵州大学, 2019.
TANG J D.Microbial community structure and screening of functional yeasts in Guizhou traditional Xiaoqu[D].Guiyang:Guizhou University, 2019.
[14] 黄岩, 张燕萍.双波长法测定糯米粉支链淀粉的含量[J].食品科技, 2014, 39(7):138-141.
HUANG Y, ZHANG Y P.Determination of amylopectin in the glutinous rice flour by dual-wavelength spectrophotometry[J].Food Science and Technology, 2014, 39(7):138-141.
[15] 谭亮, 董琦, 耿丹丹, 等.双波长比色法测定不同产地和品种青稞中直链淀粉和支链淀粉的含量[J].食品工业科技, 2015, 36(2):79-84.
TAN L, DONG Q, GENG D D, et al.Determination of amylase and amylopectin in hullessbarley from different habitats and varieties by dual-wavelength colorimetric method[J].Science and Technology of Food Industry, 2015, 36(2):79-84.
[16] WANG C X, TANG J D, QIU S Y.Profiling of fungal diversity and fermentative yeasts in traditional Chinese Xiaoqu[J].Frontiers in Microbiology, 2020, 11:2 103.
[17] 焦梦悦, 高涵, 王伟娜, 等.四种测定直链淀粉和支链淀粉方法的比较[J].食品工业科技, 2019, 40(12):259-264.
JIAO M Y, GAO H, WANG W N, et al.Comparison of four methods for the determination of amylose and amylopectin[J].Science and Technology of Food Industry, 2019, 40(12):259-264.
[18] 厦门大学化学系分析化学教研室. 双波长分光光度法原理简介[J].分析化学, 1978, 6(3):224-231.
Department of Analytical Chemistry, Department of Chemistry, Xiamen University.Brief introduction to the principle of Dual Wavelength Spectrophotometry[J].Chinese Journal of Analytical Chemistry, 1978, 6(3):224-231.
[19] 范明顺, 张崇玉, 张琴, 等.双波长分光光度法测定高粱中的直链淀粉和支链淀粉[J].中国酿造, 2008(11):85-87.
FAN M S, ZHANG C Y, ZHANG Q, et al.Determination of amylose and amylopectin content in sorghum by dual wavelength method[J].China Brewing, 2008(11):85-87.
[20] 刘姗, 王建军, 范小娟, 等.稻米直链淀粉检测技术的研究现状与展望[J].中国粮油学报, 2015, 30(3):140-146.
LIU S, WANG J J, FAN X J, et al.Status and prospects for determination for amylose content in rice[J].Journal of the Chinese Cereals and Oils Association, 2015, 30(3):140-146.
[21] 张雪梅, 张玲, 高飞虎, 等.不同分散温度、时间对双波长法测定大米中淀粉质量分数的影响研究[J].西南大学学报(自然科学版), 2020, 42(4):49-55.
ZHANG X M, ZHANG L, GAO F H, et al.The effects of different conditions (dispersion temperature and time) in dual wavelength violet spectrophotographic determination of rice starch content[J].Journal of Southwest University (Natural Science), 2020, 42(4):49-55.
[22] 马艳弘, 钟小仙, 乔月芳, 等.双波长法测定珍珠粟淀粉中直链和支链淀粉的含量[J].江苏农业科学, 2016, 44(12):331-334.
MA Y H, ZHONG X X, QIAO Y F, et al.Determination of amylose and amylopectin in pearl millet starch by dual wavelength method[J].Jiangsu Agricultural Sciences, 2016, 44(12):331-334.
[23] 黄治国, 侯海波, 罗惠波, 等.浓香型白酒酒醅发酵过程中淀粉和还原糖的变化规律研究[J].中国酿造, 2012, 31(7):107-110.
HUANG Z G, HOU H B, LUO H B, et al.Changes of starch and reducing sugars of fermented grains in fermentation of srrong-flavor liquor[J].China Brewing, 2012, 31(7):107-110.
[24] 李玉珍, 肖怀秋, 兰立新, 等.大米液态发酵过程中蛋白质及还原糖的动态变化初探[J].江苏调味副食品, 2008, 25(3):19-21.
LI Y Z, XIAO H Q, LAN L X, et al.Research on the dynamic changes of protein and reducing sugar in the process of rice fermentation with liquid-state[J].Jiangsu Condiment and Subsidiary Food, 2008, 25(3):19-21.
[25] 蒋世云. 不同淀粉质对米甜酒品质影响的研究[J].中国酿造, 2003(3):23-25.
JIANG S Y.Study on the effect of different types of starch on the quality of glutinous rice wine[J].China Brewing, 2003(3):23-25.
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