The different extractions parts from the crude and steam-processed Polygonatum cyrtonema Hua were tested for antioxidation and inhibiting α-glucosidase activities to explore potential active sites. The crude and steam-processed P. cyrtonema Hua were refluxed with 50% ethanol, and the contents of the polysaccharide, flavonoids and total phenol in extracts were determined. Then, the ethanol extracts were suspended in water and subsequently extracted with petroleum ether, ethyl acetate, n-butanol to get corresponding fractions. The antioxidant activities were evaluated by DPPH radical, ABTS radical, hydroxyl radical and superoxide anion radical methods, and vitamin C was used as a positive control. In addition, the inhibitory activity of α-glucosidase was determined by using the acarbose as the positive control. The results showed that the polysaccharide content was decreased and flavonoids and phenol content was increased. The antioxidant and hypoglycemic activities of P. cyrtonema Hua were enhanced after steam-processing. Moreover, different polar extractions parts of P. cyrtonema and steam-processed P. cyrtonema showed different degrees of antioxidant activity which as follows: Ethyl acetate phase>water phase>n-butyl alcohol phase>petroleum ether phase, alcohol extract of steam-processed P. cyrtonema >alcohol extract of P. cyrtonema, and the ethyl acetate extract of the steam-processed P. cyrtonema exhibited the strongest inhibitory activity of α-glucosidase [IC50 =(1.369±0.004) mg/mL] in vitro. The results provided a theoretical basis for P. cyrtonema as hypoglycemic food and natural antioxidants.
TENG Huanhuan
,
WANG Renzhong
,
WU Deling
,
JIN Chuanshan
,
LIU Chunfeng
,
TANG Xu
,
HUANG Shengzhuo
,
XU Fengqing
. The study on the antioxidant and hypoglycemic activities of different polar extracts from crude and steam-processed Polygonatum cyrtonema Hua[J]. Food and Fermentation Industries, 2022
, 48(8)
: 70
-75
.
DOI: 10.13995/j.cnki.11-1802/ts.030330
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