The educational concept of student-centered and building morality and cultivating people is the fundamental purpose of the current teaching reform. The traditional teaching mode is no longer suitable for the current needs of teaching and educating people, and it limits the development of the characteristic major of Vine and Wine Engineering in our university and the first-class major in Liaoning province. Therefore, it is imperative to change the previous teacher-centered teaching mode and the single assessment method, and set up the online and offline mixed teaching reform of courses. Combined with the current training objectives of grape and wine specialty, this paper explores and applies the online and offline mixed teaching in Principles of Fermentation Technology, which is the main professional courses in the major of Vine and Wine Engineering. The results showed that the online and offline mixed teaching and the increase of formative evaluation significantly improved students′ self-learning, interest and learning effect, and helped to improve students′ ability to analyze and solve problems. This provides a basis for the teaching reform of related professional courses of this major.