Food and Fermentation Industries >

2022 , Vol. 48 >Issue 9: 132 - 138

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028861

Composition analysis, antioxidant and bacteriostatic activities of volatile oil from Zanthoxylum armatum DC.

  • CHENG Si ,
  • LUO Yuan ,
  • LUO Shajie ,
  • DAI Jiangang ,
  • LI Meifeng ,
  • RAO Chaolong ,
  • CHEN Yan
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  • 1(School of Public Health, Chengdu University of Traditional Chinese Medicine, Chengdu 611130, China)
    2(School of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu 611130, China)

Received date: 2021-07-31

  Revised date: 2021-08-23

  Online published: 2022-05-26

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Abstract

The volatile oil from Zanthoxylum armatum DC. was extracted by steam distillation and analyzed by GC-MS, and the antioxidant and bacteriostatic activities were measured. Moreover, the bacteriostatic mechanism of the volatile oil was preliminarily explored by monitoring the optical density and observing the cell morphology. In this experiment, a total of 12 chemical compounds were identified from the components of the volatile oil. The relative contents of linalool (46.43%), D-limonene (26.41%), sabinene (10.84%) were higher. The results showed that the volatile oil had certain reducing ability, but the antioxidant effect was not as good as vitamin C. The DIZ of volatile oil against Escherichia coli, Enterobacter aerogenes, Salmonella enterica, Shigella castellani and Staphylococcus aureus were 11.4, 9.1, 12.7, 13.5 and 12.1 mm, and MIC were 0.1%, 1.0%, 0.1%, 0.2%, 0.2%, respectively. MBC were 0.2%, 1.0%, 0.2%, 0.2% and 0.4%, respectively. The Escherichia coli, S. castellani and S. aureus were treated with the volatile oil (1/2 MIC and MIC) and the optical density value was significantly decreased compared with the control within 12 h. The bacterial cells treated with the volatile oil were sunken and had rough surface according to SEM images. The results indicated that the volatile oil had different degrees of antibacterial effect on the five tested bacteria, which could destroy the membranes and cytoderms of the bacterial, resulting in leakage of intracellular components.

Key words： Zanthoxylum armatum DC.; volatile oil; composition; antioxidant activity; bacteriostatic activity

Cite this article

CHENG Si , LUO Yuan , LUO Shajie , DAI Jiangang , LI Meifeng , RAO Chaolong , CHEN Yan . Composition analysis, antioxidant and bacteriostatic activities of volatile oil from Zanthoxylum armatum DC.[J]. Food and Fermentation Industries, 2022 , 48(9) : 132 -138 . DOI: 10.13995/j.cnki.11-1802/ts.028861

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