Analysis of microemulsion system using thin-layer chromatographic in black  chokeberry and its bioautography for screening antioxidant components

ZHANG Tingting; BAI Fuye; LI Qiaoqiao; LI Lanlan; AN Zechong; JIANISAILIKE·Bateerhan; LI Xinxia

doi:10.13995/j.cnki.11-1802/ts.028665

2022 , Vol. 48 >Issue 9: 139 - 145

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028665

Analysis of microemulsion system using thin-layer chromatographic in black chokeberry and its bioautography for screening antioxidant components

ZHANG Tingting ,
BAI Fuye ,
LI Qiaoqiao ,
LI Lanlan ,
AN Zechong ,
JIANISAILIKE·Bateerhan ,
LI Xinxia

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(College of Pharmacy, Xinjiang Medical University, Urumqi 830011, China)

Received date: 2021-07-15

Revised date: 2021-08-02

Online published: 2022-05-26

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Abstract

A semi-quantitative method was established for the determination of various components in black chokeberry, and its antioxidant capacity and antioxidant active components were determined. High-performance thin-layer chromatography was used to analyze the cyanidin-3-O-glucoside, hyperoside and chlorogenic acid of black chokeberry simultaneously, with 75% microemulsion-formic acid (9:1) as the eluent. The methods of DPPH free radicals and thin layer chromatography (TLC)-bioautography technique were used to determine its antioxidant capacity of black chokeberry. The results showed that the TLC analysis method was good for the detection of microemulsion system. The determination of DPPH scavenging ability showed that the IC₅₀ of vitamin C was 13.5 μg/mL, while black chokeberry was 0.18 mg/mL. TLC-bioautography results showed that cyanidin-3-O-glucoside, cyanidin-3-O-arabinoside, hyperoside, chlorogenic acid and neochlorogenic acid, all of which had antioxidant activity. The established high-efficiency thin-layer scanning method was reproducible, stable and could be used as a simple and rapid semi-quantitative method to determine the content of chemical components in black chokeberry. The determination of antioxidant activity and the recognition of the composition of black chokeberry provides the basis for follow-up studies.

Key words： black chokeberry; multi-ingredient; microemulsion system-thin layer chromatography; bioautography; antioxidant activity

Cite this article

ZHANG Tingting , BAI Fuye , LI Qiaoqiao , LI Lanlan , AN Zechong , JIANISAILIKE·Bateerhan , LI Xinxia . Analysis of microemulsion system using thin-layer chromatographic in black chokeberry and its bioautography for screening antioxidant components[J]. Food and Fermentation Industries, 2022 , 48(9) : 139 -145 . DOI: 10.13995/j.cnki.11-1802/ts.028665

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