To investigate the effect of coating on chilling injury of cucumber during low-temperature storage, the following indicators were regularly detected and analyzed, which including chilling injury index, chilling injury rate, weight loss rate, decay rate, respiration rate, firmness, ascorbic acid content, antioxidant enzymes activities [superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT) and glutathione reductase (GR)], superoxide anion production (·O2-), H2O2 content, malondialdehyde (MDA) accumulation and membrane relative permeability of chitosan-nano-ZnO, melatonin and chitosan-nano-ZnO-melatonin composite. The results showed that chitosan-nano-ZnO, melatonin and chitosan-nano-zinc oxide-melatonin composite coating could significantly reduce the chilling injury index and chilling injury rate, maintain low weight loss rate and decay rate and delay respiration rate. Meanwhile, it also significantly inhibited the decrease of firmness and ascorbic acid content and increased the activities of SOD, APX, CAT and GR. Besides, the rate of ·O2- production and H2O2 content were also reduced and MDA accumulation and membrane relative permeability were delayed. These results showed that above three treatments could reduce chilling injury and prolong the storage period of cucumber, and chitosan-nano-ZnO-melatonin composite coating had the best effect. This study will provide a theoretical basis for alleviation of chilling injury of cucumber.
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