Food and Fermentation Industries >

2022 , Vol. 48 >Issue 9: 230 - 234

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.029053

Enumeration and MALDI-TOF MS identification of contaminating bacteria in foods containing lactic acid bacteria

  • LING Li ,
  • LYU Jingzhang ,
  • CHEN Jing ,
  • JIANG Yue ,
  • WAN Zhigang ,
  • LIU Jingwen ,
  • YAN Qiongying ,
  • LU Xingan
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  • 1(Guangzhou Customs Technology Center, Guangzhou 510623, China)
    2(Shenzhen Academy of Inspection and Quarantine, Shenzhen 518045, China)
    3(Food Inspection and Quarantine Technology Center of Shenzhen Customs District, Shenzhen 518045, China)
    4(Shenzhen Academy of Metrology and Quality Inspection, Shenzhen 518131, China)
    5(Guangdong Pharmaceutical University, Zhongshan 528045, China)
    6(Chinese Academy of Inspection and Quarantine,Beijing 100176, China)

Received date: 2021-08-18

  Revised date: 2021-09-23

  Online published: 2022-05-26

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Abstract

To enumerate and identify the contaminating bacteria in foods containing lactic acid bacteria(LAB), MRS agar was used to enumerate the LAB in six starter powders and 12 kinds of commercial LAB-containing foods. Meanwhile, total aerobic microorganisms and non-LAB were enumerated by plate counting agar (PCA) and count agar sugar free (CASF) respectively. The colonies on PCA and CASF plates were identified by matrix-assisted laser desorption/ ionization time of flight mass spectrometry (MALDI-TOF MS). In starter powders, the numbers of LAB ranged from 3.2×1010-4.7×1010 CFU/g and no colony was found on PCA and CASF plates. In all commercial foods containing LAB, the numbers of LAB were higher than 106 CFU/g. After identified by MALDI-TOF MS, the colonies on the PCA plates were proved to contain both LAB and non-LAB while the colonies on the CASF plates were identified as non-LAB. The non-LAB belonged to the contaminating bacteria in LAB foods. Based on these results, this study revealed that the contaminating bacteria in commercial foods containing LAB can be enumerated by CASF and identified by MALDI-TOF MS quickly and accurately.

Key words： food containing lactic acid bacteria; contaminating bacteria; count agar sugar free(CASF); matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDT-TOF MS)

Cite this article

LING Li , LYU Jingzhang , CHEN Jing , JIANG Yue , WAN Zhigang , LIU Jingwen , YAN Qiongying , LU Xingan . Enumeration and MALDI-TOF MS identification of contaminating bacteria in foods containing lactic acid bacteria[J]. Food and Fermentation Industries, 2022 , 48(9) : 230 -234 . DOI: 10.13995/j.cnki.11-1802/ts.029053

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