The heat and oxidative tolerance of Lactobacillus rhamnosus hsryfm 1301 was strengthened in low nitrogen source environment, but it was unknown whether low nitrogen source would affect the tolerance of L. rhamnosus hsryfm 1301 to other stresses. The influence of nitrogen sources on the stress tolerance of L. rhamnosus was investigated. It was shown that the thermotolerance and aerotolerance of L. rhamnosus hsryfm 1301 were further strengthened by low nitrogen source environment and cross-adaptation. Under low nitrogen source condition, the thermotolerance and aerotolerance of L. rhamnosus hsryfm 1301 increased by 129 and 40 times, respectively, during the exponential phase, while its tolerance to other common stresses such as acid stress, bile salt stress, freezing stress and osmotic stress, was not influenced. It was suggested that low nitrogen source environment and cross-adaptation could benefit the tolerance of L. rhamnosus to heat stress and oxidative stress during production processes such as spray drying.
WANG Dai
,
HAN Yuemei
,
GUI Ya
,
YU Xingtong
,
WA Yunchao
,
GU Ruixia
,
ZHANG Chenchen
. Influence of nitrogen sources on the stress tolerance of Lactobacillus rhamnosus[J]. Food and Fermentation Industries, 2022
, 48(10)
: 78
-83
.
DOI: 10.13995/j.cnki.11-1802/ts.029268
[1] 陈卫. 乳酸菌科学与技术[M].北京:科学出版社, 2018.
CHEN W.Science and Technology of Lactic Acid Bacteria[M].Beijing:Science Press, 2018.
[2] TRIPATHI M K, GIRI S K.Probiotic functional foods:Survival of probiotics during processing and storage[J].Journal of Functional Foods, 2014, 9:225-241.
[3] KARIMI R, MORTAZAVIAN A M, DA CRUZ A G.Viability of probiotic microorganisms in cheese during production and storage:A review[J].Dairy Science and Technology, 2011, 91(3):283-308.
[4] PAPADIMITRIOU K, ALEGRÍA Á, BRON P A, et al.Stress physiology of lactic acid bacteria[J].Microbiology and Molecular Biology Reviews, 2016, 80(3):837-890.
[5] CHEN D W, YANG Z Q, CHEN X, et al.Effect of Lactobacillus rhamnosus hsryfm 1301 on the gut microbiota and lipid metabolism in rats fed a high-fat diet[J].Journal of Microbiology and Biotechnology, 2015, 25(5):687-695.
[6] ZHANG C C, LU J Y, YANG D, et al.Stress influenced the aerotolerance of Lactobacillus rhamnosus hsryfm 1301[J].Biotechnology Letters, 2018, 40(4):729-735.
[7] ZHANG C C, GUI Y, CHEN X, et al.Transcriptional homogenization of Lactobacillus rhamnosus hsryfm 1301 under heat stress and oxidative stress[J].Applied Microbiology Biotechnology, 2020, 104(4):2 611-2 621.
[8] YANG H, HE M W, WU C D.Cross protection of lactic acid bacteria during environmental stresses:Stress responses and underlying mechanisms[J].LWT, 2021, 144:111203.
[9] ZHANG C C, YU X T, WANG D X, et al.Rapid strain-specific identification of two Lactobacillus rhamnosus strains using PCR based on gene family analysis[J].LWT, 2021, 146(2):111395.
[10] ZHANG C, YANG L, DING Z, et al.New selective media for isolation and enumeration of Lactobacillus rhamnosus and Streptococcus thermophilus[J].Journal of Food Measurement and Characterization, 2019, 13:1 431-1 439.
[11] JEWELL J B, KASHKET E R.Osmotically regulated transport of proline by Lactobacillus acidophilus IFO 3532[J].Applied and Environmental Microbiology, 1991, 57(10):2 829-2 833.
[12] CALDERINI E, CELEBIOGLU H U, VILLARROEL J, et al.Comparative proteomics of oxidative stress response of Lactobacillus acidophilus NCFM reveals effects on DNA repair and cysteine de novo synthesis[J].Proteomics, 2017, 17(5):1600178.
[13] HUNG J, COOPER D, TURNER M S, et al.Cystine uptake prevents production of hydrogen peroxide by Lactobacillus fermentum BR11[J].FEMS Microbiology Letters, 2003, 227(1):93-99.
[14] WANG G H, ZHAI Z Y, REN F Z, et al.Combined transcriptomic and proteomic analysis of the response to bile stress in a centenarian-originated probiotic Lactobacillus salivarius Ren[J].Food Research International, 2020, 137:109331.
