Preparing and consuming home-made Paocai is a tradition in Chongqing. The pellicle (biofilm) formation in Paocai brines is a common problem during fermentation. In order to study the fungi related to pellicle, Illumina MiSeq sequencing was used to sequence the ITS1 genes of the Paocai brine samples with (P group) and without (NP group) pellicle, the differences in fungal community structures between the two groups were investigated. The major genera of group P included Pichia (85.22%), Candida (2.07%), Aspergillus (1.54%) and Fusarium (1.25%). The major genera of NP group included Pichia (46.04%), Debaryomyces (21.75%), Aspergillus (9.57%), Kazachstania (5.31%), Simplicillium (2.72%), Penicillium (1.43%), Wickerhamomyces (1.32%), Fusarium (1.15%), Candida (1.04%) and Schwanniomyces (1.03%). The average biomass of fungi in group P was almost 20 times than that of group NP. Significant differences were observed in the relative abundance of genus Debaryomyces and Kazachstania by Kruskal Wallis test. This study demonstrates the fungal community structure information related to pellicle phenomenon, which is helpful for preventing and controlling pellicle in home-made Paocai.
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