Food and Fermentation Industries >

2022 , Vol. 48 >Issue 11: 101 - 106

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.029888

Oligosaccharide utilization characteristics of lactic acid bacteria from breast milk

  • WANG Peng ,
  • GANG Mengxuan ,
  • ZHANG Chenchen ,
  • YAN Haodong ,
  • PAN Lina ,
  • KANG Wenli ,
  • WANG Jiaqi ,
  • DAI Zhiyong ,
  • GU Ruixia ,
  • CHEN Xia
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  • 1(Jiangsu Key Laboratory of Dairy Biotechnology and Safety Control, Yangzhou University, Yangzhou 225127, China)
    2(Ausnutria Institute of Food and Nutrition, Ausnutria Dairy (China) Co, Ltd, Changsha 410000, China)
    3(Jiangsu Dairy Biotechnology Engineering Research Center, Yangzhou 225004, China)
    4(Key Laboratory of Probiotics and Dairy Deep Processing of Yangzhou University, Yangzhou 225127, China)

Received date: 2021-10-30

  Revised date: 2021-11-16

  Online published: 2022-06-23

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Abstract

In order to explore the utilization of oligosaccharides by lactic acid bacteria from breast milk, the growth ability of eight lactic acid bacteria strains in media with different oligosaccharides was compared. The results showed that fructo-oligosaccharide and inulin could promote the growth of four Lactobacillus paracasei strains (compared with glucose); fructo-oligosaccharide and galacto-oligosaccharide could significantly promote the growth of Lactobacillus plantarum M113; Lactobacillus rhamnosus M53 had better utilization of galacto-oligosaccharides than others; two Bifidobacterium strains had stronger utilization of galacto-oligosaccharide and stachyose. None of the tested lactic acid bacteria could make better use of the common 2'-fucosyllactose in human milk and xylo-oligosaccharide. These lactic acid bacteria had strain specificity in the utilization of oligosaccharides. The growth ability of strains in mixed oligosaccharides depends on the content of their preferred oligosaccharides. The results provide data support for the screening of probiotics and oligosaccharides in infant formula milk powder.

Key words： lactic acid bacteria from breast milk; oligosaccharides; inulin; utilization characteristics

Cite this article

WANG Peng , GANG Mengxuan , ZHANG Chenchen , YAN Haodong , PAN Lina , KANG Wenli , WANG Jiaqi , DAI Zhiyong , GU Ruixia , CHEN Xia . Oligosaccharide utilization characteristics of lactic acid bacteria from breast milk[J]. Food and Fermentation Industries, 2022 , 48(11) : 101 -106 . DOI: 10.13995/j.cnki.11-1802/ts.029888

References

[1] 沙丽君, 李晓南. 人乳成分与儿童生长发育[J]. 中国实用儿科杂志, 2019, 34(10): 838-841.
SHA L J, LI X N. Human milk composition and children's growth and development[J]. Chinese Journal of Practical Pediatrics, 2019, 34(10): 838-841.
[2] 刘萌颖. 婴儿配方奶粉中低聚糖对肠道益生功能的影响[D]. 哈尔滨: 东北农业大学, 2018.
LIU M Y. Effect of oligosaccharide in infant formula on intestinal probiotic function[D]. Harbin: Northeast Agricultural University, 2018.
[3] GUARALDI F, SALVATORI G. Effect of breast and formula feeding on gut microbiota shaping in newborns[J]. Frontiers in Cellular and Infection Microbiology, 2012, 2: 94.
[4] 赵洁, 孙天松. 母乳对婴儿肠道菌群及免疫系统影响的研究进展[J]. 食品科学, 2017, 38(1): 289-296.
ZHAO J, SUN T S. Recent advances of the role of human breast milk in the development of the intestinal microbiota and immune system of infants[J]. Food Science, 2017, 38(1): 289-296.
[5] MASTROMARINO P, CAPOBIANCO D, CAMPAGNA G, et al. Correlation between lactoferrin and beneficial microbiota in breast milk and infant's feces[J]. BioMetals, 2014, 27(5):1 077-1 086.
[6] 袁慧芝, 荀一萍, 蒲晓璐, 等. 母乳低聚糖与婴儿肠道菌群相关性研究进展[J]. 食品科学, 2021, 42(13):319-325.
YUAN H Z, XUN Y P, PU X L, et al. Progress in understanding the relationship between human milk oligosaccharides and infant gut microbiota[J]. Food Science, 2021, 42(13):319-325.
[7] BOUDRY G, CHARTON E, LE HUEROU-LURON I, et al. The relationship between breast milk components and the infant gut microbiota[J]. Frontiers in Nutrition, 2021, 8:629740.
[8] BODE L. Human milk oligosaccharides: Every baby needs a sugar mama[J]. Glycobiology, 2012, 22(9): 1 147-1 162.
[9] ZHANG B, LI L Q, LIU F T, et al. Human milk oligosaccharides and infant gut microbiota: Molecular structures, utilization strategies and immune function[J]. Carbohydrate Polymers, 2022, 276:118738.
[10] SMILOWITZ J T, LEBRILLA C B, MILLS D A, et al. Breast milk oligosaccharides: Structure-function relationships in the neonate[J]. Annual Review of Nutrition, 2014, 34: 143-169.
[11] ABOULOIFA H, KHODAEI N, ROKNI Y, et al. The prebiotics (fructo-oligosaccharides and xylo-oligosaccharides) modulate the probiotic properties of Lactiplantibacillus and Levilactobacillus strains isolated from traditional fermented olive[J]. World Journal of Microbiology & Biotechnology, 2020, 36(12): 185.
[12] 张鹏宇, 张美萍, 麻开香, 等. 几种常用益生元不同配伍对三株益生菌体外生长的调节作用[J]. 中国微生态学杂志, 2019, 31(1): 21-24.
ZHANG P Y, ZHANG M P, MA K X, et al. The effects of commonly used prebiotics on the growth of three strains of probiotics in vitro[J]. Chinese Journal of Microecology, 2019, 31(1): 21-24.
[13] ENDO H, TAMURA K, FUKASAWA T, et al. Comparison of fructooligosaccharide utilization by Lactobacillus and Bacteroides species[J]. Bioscience, Biotechnology, and Biochemistry, 2012, 76(1): 176-179.
[14] LANGA S, VAN DEN BULCK E, PEIROTÉN A, et al. Application of lactobacilli and prebiotic oligosaccharides for the development of a synbiotic semi-hard cheese[J]. LWT, 2019, 114:108361.
[15] 陈韫慧, 方思璇, 陈佳琪, 等. 不同益生元对植物乳杆菌生长的影响[J]. 食品与发酵工业, 2020, 46(21):28-33.
CHEN Y H, FANG S X, CHEN J Q, et al. Effects of different prebiotics on the growth of Lactobacillus plantarum[J]. Food and Fermentation Industries, 2020, 46(21):28-33.
[16] IGNATOVA T, ILIEV I, KIRILOV N, et al. Effect of oligosaccharides on the growth of Lactobacillus delbrueckii subsp. bulgaricus strains isolated from dairy products[J]. Journal of Agricultural and Food Chemistry, 2009, 57(20):9 496-9 502.
[17] MATSUKI T, YAHAGI K, MORI H, et al. A key genetic factor for fucosyllactose utilization affects infant gut microbiota development[J]. Nature Communications, 2016, 7(1): 11939.
[18] AUTRAN C A, SCHOTERMAN M H C, JANTSCHER-KRENN E, et al. Sialylated galacto-oligosaccharides and 2′-fucosyllactose reduce necrotising enterocolitis in neonatal rats[J]. The British Journal of Nutrition, 2016, 116(2):294-299.
[19] 邬佳颖, 陈敏暄, 金天赐, 等. 双歧杆菌和乳杆菌对水苏糖的利用特性[J]. 食品与发酵工业, 2021, 47(24):13-20.
WU J Y, CHEN M X, JIN T C, et al. Utilization characteristics of stachyose by Bifidobacterium and Lactobacillus[J]. Food and Fermentation Industries, 2021, 47(24):13-20.
[20] 卫生部. 关于公布可用于婴幼儿食品的菌种名单的公告[J]. 中国食品卫生杂志, 2011, 23(6): 594.
Ministry of Health. Announcement on the announcement of the list of bacterial strains that can be used in food for infants and young children[J]. Chinese Journal of Food Hygiene, 2011, 23(6): 594.
[21] GOPAL P K, SULLIVAN P A, SMART J B. Utilisation of galacto-oligosaccharides as selective substrates for growth by lactic acid bacteria including Bifidobacterium lactis DR10 and Lactobacillus rhamnosus DR20[J]. International Dairy Journal, 2001, 11(1-2):19-25.
[22] WATSON D, O'CONNELL-MOTHERWAY M, SCHOTERMAN M H C, et al. Selective carbohydrate utilization by lactobacilli and bifidobacteria[J]. Journal of Applied Microbiology, 2013, 114(4): 1 132-1 146.
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