Microbial extracellular polysaccharides possess various beneficial properties and are potential prebiotic. The extracellular polysaccharide BPS-2 was extracted from Bacillus thuringiensis IX-01 by high-density cellular fermentation. BPS-2 is a heteropolysaccharide composed of aminogalactose, arabinose, glucosamine, glucose and mannose with a molar percentage of 5.53∶1.77∶4.74∶3.24∶1. It has a polysaccharide molecular mass of 27.96 kDa. MTT assay was used to evaluate the cytotoxicity of BPS-2, and microorganisms from 10 healthy human fecal were obtained to investigate the effect of BPS-2 on the composition and metabolites of human fecal flora under the premise that its polysaccharide was not cytotoxic. During 48 h of static fermentation in vitro, BPS-2 significantly enhanced the production of short-chain fatty acids compared to the negative control (P<0.05), with concentrations of acetic acid, propionic acid, butyric acid and total short-chain fatty acids reaching (31.59±0.73), (10.82±0.65), (8.18±0.2), (50.59±1.54) and (50.59±1.54) mmol/L, respectively. Sequencing of 16S rRNA gene showed that BPS-2 significantly increased the relative abundance of the beneficial genus Parabacteroides and reduced the relative abundance of the harmful microflora Megamonas and Fusobacterium, which in turn were involved in improving the structure of the host gut microbiota. This is the first report on in vitro probiotic properties of BPS-2 produced by B. thuringiensis, which can provide a basis for its application in the food industry.
GAO Zexin
,
SUN Wu
,
XU Lingming
,
ZHANG Leilei
,
ZHU Li
,
ZHAN Xiaobei
. Probiotic properties of Bacillus thuringiensis IX-01 extracellular polysaccharide in vitro[J]. Food and Fermentation Industries, 2022
, 48(12)
: 1
-8
.
DOI: 10.13995/j.cnki.11-1802/ts.030184
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