This study aimed to improve the resistance of Lactobacillus plantarum to cephalexin by laboratory evolution, and to evaluate the probiotic properties of resistant strains. At the same time, whole-genome sequencing was used to detect gene mutations of evolved strains. When L. plantarum evolved in the presence of cephalexin, its minimum inhibitory concentration (MIC) increased from the original 16 μg/mL to 8 192 μg/mL, and the resistance increased by 512 times. When cephalexin was removed and L. plantarum further evolved, its MIC value reduced from 8 192 μg/mL to 4 096 μg/mL, but it remained stable during the subsequent evolution. Compared with the original strain, its resistance increased by 256 times, indicating that L. plantarum with high resistance could be obtained through laboratory evolution. The study on the probiotic properties of highly resistant L. plantarum showed that the acid tolerance, bile salts tolerance and adhesion ability of the strains did not change significantly after evolution, indicating that probiotic properties of the evolved strain were stable. Whole genome sequencing of the strains also detected mutant genes related to resistance and probiotic properties. The results provide a reference for the combined use of L. plantarum and antibiotics.
WU Amin
,
ZHANG Fuxin
,
WU Shan
,
HE Chen
. The resistance evolution of Lactobacillus plantarum to cephalexin and its probiotic properties evaluation[J]. Food and Fermentation Industries, 2022
, 48(12)
: 90
-96
.
DOI: 10.13995/j.cnki.11-1802/ts.029592
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