Previous studies confirmed that the metal antimicrobial peptide SIF4 had good antimicrobial activity against Staphylococcus aureus, and the changes of internal and external factors of food had little effect on the relative antimicrobial activity (RAMA) and had good biocompatibility. In this study, adopting S. aureus as the indicative bacterium, the antimicrobial stability (AMS) of SIF4 in several simulated single/multiple food systems that constructed using protein, lipid and starch as the matrix were investigated. Results showed that SIF4 had good AMS in simulated protein systems established by bovine whey protein (BWP), soybean protein isolate (SPI) or peanut protein isolate (PPI). Rapeseed oil (RO), peanut oil (PO) and soybean oil (SO) lipid systems could synergistically enhance RAMA. SIF4 also had good AMS in simulated starch systems, such as rice starch system, potato starch system and corn starch system. There was no significant difference for the RAMA of SIF4 between the experimental group and control group (P>0.05) in simulated BWP-SO system, and the RAMA of the experimental group was significantly higher than the control group in BWP-PO system (P<0.05), but for the simulated BWP-RO system, significant difference was only detected when the BWP∶RO were 0.5∶1 and 1∶1(P>0.05). The RAMA of the experimental group was significantly higher than that of the control in simulated SPI-RO, SPI-SO and SPI-PO system (P<0.05). SIF4 could maintain good AMS or be enhanced with different extents in PPI-RO, PPI-SO and PPI-PO simulated system. There was no significant difference, for RAMA, between the experimental group and the control group in simulated RS-BWP system. When RS:SPI and RS:PPI were 1∶1 or 1∶2, the RAMA of the experimental group was significantly higher than that of the control (P<0.05) in simulated RS-SPI and RS-PPI system. There was no significant difference for RAMA in simulated PS-BWP、PS-SPI, PS-PPI, CS-BWP, CS-SPI and CS-PPI simulated system. SIF4 could maintain good AMS or RAMA, and could be enhanced in simulated RS-RO, RS-SO, RS-PO, PS-RO, PS-SO and PS-PO simulated system, but no significant difference was detected between the experimental group and the control in simulated CS-RO, CS-SO and CS-PO system. SIF4 also posed good AMS in simulated protein-starch-lipid system. All results indicated that SIF4 had good AMS or could be enhanced in tested simulated food systems, and can be used as a potential antimicrobial preservative agent in complex food systems.
LI Yuzhen
,
XIAO Huaiqiu
,
CAO Dan
,
WANG Lin
,
ZENG Mengqi
,
LIN Qinlu
,
ZHAO Mouming
. Antimicrobial stability of metal antimicrobial peptide SIF4 in simulated single or multiple component food systems[J]. Food and Fermentation Industries, 2022
, 48(12)
: 131
-137
.
DOI: 10.13995/j.cnki.11-1802/ts.030922
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