Osmotolerant yeast isolates recovered from honey samples were purified and their 26S rDNA, glucose assays, and growth curves were analyzed. The correlation analysis was performed among physicochemical properties and osmotolerant yeast characteristics. To develop a new method for detecting the osmotolerant yeast in honey in early contamination, headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) was evaluated for diagnosis of the volatile compounds (VOCs) fingerprints variation during early contamination in jujube honey and acacia honey. The results indicated that the physicochemical properties of the 35 honey samples were: pH 3.45-4.48, moisture content (16.91±0.09)%-(20.82±0.20)%, and total sugar concentration 62.01%-76.54%. The total contamination rate in honey was 20.0% (7/35), and it exhibited significant positive correlation with moisture content (P<0.01). Seven osmotolerant yeast strains were all Zygosaccharomyces sp., and tolerated to 800 g/L glucose. A total of 54 and 35 VOCs were detected by HS-GC-IMS analysis in jujube honey and acacia honey, respectively. Most of them were aldehydes and ketones, followed by esters and alcohols. The furfurol, furfurol dimmer, benzaldehyde, benzaldehyde dimmer, cyclohexanone and 2-heptanone could be used as typical flavor substances to distinguish the yeast contamination at the early stage (3-7 d) in honey. Overall, HS-GC-IMS performed as an effective technique in VOCs typing after osmotolerant yeast early stage (3-7 d) contamination in honey, and exhibited great potential in honey osmotolerant yeast early detection as its sensitive, rapid, and convenient properties.
WANG Yin
,
LIU Cailing
,
WANG Xian
,
YUE Tianli
,
CAO Wei
. Characterization of osmotolerant yeasts and the early detection based on HS-GC-IMS in honey[J]. Food and Fermentation Industries, 2022
, 48(12)
: 267
-274
.
DOI: 10.13995/j.cnki.11-1802/ts.031144
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