Food and Fermentation Industries >

2022 , Vol. 48 >Issue 13: 33 - 41

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.029907

Effect of betaine phosphate on polymalic acid fermentation by Aureobasidium melanogenum

  • YANG Donglin ,
  • LI Jiaqian ,
  • LU Liang ,
  • WANG Genan ,
  • WANG Ji ,
  • ZHAO Tingbin ,
  • YIN Haisong ,
  • QIAO Changsheng
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  • 1(College of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457, China)
    2(Tianjin Huizhi Baichuan Bioengineering Co., Ltd., Tianjin 300457, China)
    3(College of Bioengineering, Tianjin Modern Vocational Technology College, Tianjin 300457, China)
    4(Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education and Tianjin Key Laboratory of Industrial Microbiology, Tianjin 300457, China)
    5(Tianjin Engineering Center of Microbial Metabolism and Fermentation Process Control, Tianjin 300457, China)

Received date: 2021-11-01

  Revised date: 2021-12-09

  Online published: 2022-08-03

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Abstract

Polymalic acid (PMLA) is an organic biopolymer material mainly synthesized by microorganisms, which can be used in biopharmaceuticals, degradable materials and other industries. In this study, the effect of betaine phosphate on the fermentation of polymalic acid by Aureobasidium melanogenum was investigated. The results showed that, under the optimum conditions of 8 L/min ventilation, 450 r/min constant speed, and the optimum concentration of betaine phosphate 0.9 g/L, the final polymalic acid yield was 53.10 g/L, while that in blank group was 17.29 g/L. Transcriptome analysis showed that the expression levels of key enzymes such as hexose kinase and phosphoenolpyruvate carboxykinase were increased. This study provides a feasible solution for the industrial production of polymalic acid and provides a reference for the application of betaine phosphate in other fermentation fields.

Key words： Aureobasidium melanogenum; polymalic acid; betaine phosphate; process optimization; transcriptomics

Cite this article

YANG Donglin , LI Jiaqian , LU Liang , WANG Genan , WANG Ji , ZHAO Tingbin , YIN Haisong , QIAO Changsheng . Effect of betaine phosphate on polymalic acid fermentation by Aureobasidium melanogenum[J]. Food and Fermentation Industries, 2022 , 48(13) : 33 -41 . DOI: 10.13995/j.cnki.11-1802/ts.029907

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