Amino acid composition, antioxidant activity and emulsion properties of quinoa prolamine

  • LIU Le ,
  • LI Yan ,
  • LIAN Jiada ,
  • ZHANG Ying ,
  • ZHENG Yajun
Expand
  • (Food Science College, Shanxi Normal University, Taiyuan 030092, China)

Received date: 2022-03-13

  Revised date: 2022-04-11

  Online published: 2022-08-03

Abstract

Quinoa is a new crop that is rich in protein and introduced into China in recent years.Quinoa has great potential for its high content of prolamine.But the data referring to quinoa prolamine is limited.In this study, prolamine was extracted from defatted quinoa powder.The SDS-PAGE analysis was used to investigate the molecular and subunits composition of quinoa prolamine.Moreover, the amino acid composition, antioxidant activity and emulsion properties of quinoa prolamine were studied.The results showed that the molecular weight of quinoa prolamine was about 9.74 kDa and the total essential amino acid of quinoa prolamine was 20.36 g/100 g, which was higher than that required by FAO/WHO.Moreover, quinoa prolamine showed a high chelating ability of Fe2+ (83.60% at 0.3 mg/mL), and certain scavenging activity on superoxide radical and hydroxyl radical.Quinoa prolamine also had strong reducing power and scavenging ability of ABTS cation radical (89.60%).In addition, quinoa prolamine demonstrated high emulsifying capacity index (943.32 m2/g) and emulsion stability (54.68%).These results demonstrated that quinoa prolamine had a balanced amino acid composition, high antioxidant activity and emulsion properties.Besides, quinoa prolamine had a high annual yield.Therefore, quinoa prolamine has the potential to be developed as natural nutritional supplement, antioxidant and emulsifier.These results provided theoretical basis for the development and utilization of quinoa prolamine.

Cite this article

LIU Le , LI Yan , LIAN Jiada , ZHANG Ying , ZHENG Yajun . Amino acid composition, antioxidant activity and emulsion properties of quinoa prolamine[J]. Food and Fermentation Industries, 2022 , 48(13) : 181 -187 . DOI: 10.13995/j.cnki.11-1802/ts.031525

References

[1] 于嘉淇, 王启明, 李薇雨, 等.玉米淀粉对麦醇溶蛋白凝胶特性的影响[J].食品与发酵工业, 2022, 48(2):131-138.
YU J Q, WANG Q M, LI W Y, et al.Effect of corn starch on gelatin properties of gliadin[J].Food and Fermentation Industries, 2022, 48(2):131-138.
[2] 马劲, 陈世乾, 闵建华.醇溶蛋白应用研究进展[J].中国食品添加剂, 2021, 32(3):106-112.
MA J, CHEN S Q, MIN J H.Research progress in the application of gliadin[J].China Food Additives, 2021, 32(3):106-112.
[3] HESAM D, RAMAN A, AKRAM P, et al.Quinoa bioactive protein hydrolysate produced by pancreatin enzyme:Functional and antioxidant properties[J].LWT-Food Science and Technology, 2021, 150(4):10143.
[4] DAKHILI S, ABDOLALIZADEH L, HOSSEINI S M, et al.Quinoa protein:Composition, structure and functional properties[J].Food Chemistry, 2019, 299:125161.
[5] NAVARRO-LISBOA R, HERRERA C, ZUNIGA RN, et al.Quinoa proteins (Chenopodium quinoa Willd.) fractionated by ultrafiltration using ceramic membranes:The role of pH on physicochemical and conformational properties[J].Food Bioproduce Process, 2017, 102:20-30.
[6] ZHAO Y, CHEN F, GAO C, et al.Structure, physical and antioxidant properties of quinoa protein/hsian-tsao gum composite biodegradable active films[J].LWT-Food Science and Technology, 2022, 155:112985.
[7] ZHENG Y J, WANG X, ZHANG Y L, et al.Isolation of novel ACE-inhibitory and antioxidant peptides from quinoa bran albumin assisted with an in silico approach:Characterization, in vivo antihypertension, and molecular docking[J].Molecules, 2019, 24(24):4562.
[8] BRADFORD M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding[J].Analytical Biochemistry, 1976, 72:248-254.
[9] SAHNI P, SHARMAS S, SURASANI V K R.Influence of processing and pH on amino acid profile, morphology, electrophoretic pattern, bioactive potential and functional characteristics of alfalfa protein isolates[J].Food Chemistry, 2020, 333:127503.
[10] HU S, YUAN J, GAO J, et al.Antioxidant and anti-inflammatory potential of peptides derived from the in vitro gastrointestinal digestion of germinated and heat-treated foxtail millet (Setaria italica) proteins[J].Journal of Agricultural and Food Chemistry, 2020, 35:9 415-9 426.
[11] 张方艳, 张雯雯, 朱桂兰, 等.水提法提取荸荠多糖及其体外抗氧化活性研究[J].食品与发酵工业, 2022, 48(1):104-110.
ZHANG F Y, ZHANG W W, ZHU G L, et al.Study on water extraction of polysaccharide of Chinese water chestnut and its antioxidant characters in vitro[J].Food and Fermentation Industries, 2022, 48(1):104-110.
[12] CHAI T T, XIAO J, MOHANA S D, et al. Identification of antioxidant peptides derived from tropical jackfruit seed and investigation of the stability profiles[J].Food Chemistry, 2021, 340:127876.
[13] 刘皓涵,钟迪颖,张润光, 等.欧李多酚提取纯化及抗氧化性研究[J].农业工程学报, 2020, 36(22):324-332.
LIU H H, ZHONG D Y, ZHANG R G, et al.Extraction, purification and anti-oxidation properties of European plum polyphenols[J].Transactions of the Chinese Society of Agricultural Engineering, 2020, 36(22):324-332.
[14] FAO/WHO Nutrition Meetings, Report Series 51.Protein quality evaluation[R].Rome:Food and Agricultural Organization/World Health Organization, 1990.
[15] 姬中伟. 小米醇溶蛋白肽的制备及其抗氧化与抗炎活性研究[D].无锡:江南大学, 2020.
JI Z W.Preparation of millet gliadin peptide and its antioxidant and anti-inflammatory activity[D].Wuxi:Jiangnan University, 2020.
[16] 李丹, 朱蕊芳, 张东杰.玉米醇溶蛋白水解物的Zn2+螯合能力及抗氧化活性分析[J].黑龙江八一农垦大学学报, 2020, 32(1):46-52.
LI D, ZHU R F, ZHANG D J.Analysis of Zn2+ chelating ability and antioxidant activity of zein hydrolysate[J].Journal of Heilongjiang Bayi Land Reclamation University, 2020, 32(1):46-52.
[17] CHEN X, ZHANG, T Y, WU, Y C, et al.Foxtail millet prolamin as an effective encapsulant deliver curcumin by fabricating caseinate stabilized composite nanoparticles[J].Food Chemistry, 2021, 367:3130764.
[18] SONG R, LIANG T, SHEN Q, et al.The optimization of production and characterization of antioxidant peptides from protein hydrolysates of Agrocybe aegerita[J].LWT-Food Science and Technology, 2020, 134:109987.
[19] 王岩, 王建宇, 于璐, 等.响应面法优化玉米醇溶蛋白提取工艺及抗氧化活性评价[J].东北农业大学学报, 2021, 52(4):55-64.
WANG Y, WANG J Y, YU L, et al.Optimization of zein extraction process and evaluation of antioxidant activity by response surface methodology[J].Journal of Northeast Agricultural University, 2021, 52(4):55-64.
[20] 孙烨, 李英浩, WULANDARI, 等.超声波预处理对玉米醇溶蛋白结构及其Pickering乳液稳定性的影响[J].食品与发酵工业, 2021, 47(1):97-106.
SUN Y, LI Y H, WULANDARI, et al.Ultrasonic pretreatment improves the stability of zein and Pickering emulsion[J].Food and Fermentation Industries, 2021, 47(1):97-106.
[21] 戴媛,冷进松,陆红佳.豌豆蛋白质提取工艺优化及其乳化性和起泡性研究[J].湖北农业科学, 2021, 60(8):134-140.
DAI Y, LENG J S, LU H J.Optimization of pea protein extraction process and its emulsification and foaming properties[J].Hubei Agricultural Sciences, 2021, 60(8):134-140.
[22] 李超, 樊成, 刘宁, 等.紫苏球蛋白乳化性及其O/W乳液的稳定性研究[J].食品与发酵工业, 2022, 48(2):176-181.
LI C, FAN C, LIU N, et al.Emulsifying properties and O/W emulsion stability of Perilla globulin[J].Food and Fermentation Industries, 2022, 48(2):176-181.
Outlines

/